Cars microscopic imaging device and method based on tapered fiber optic probe excitation

Abstract

The invention discloses a CARS microscopic imaging device and method based on tapered optical fiber probe excitation. The device at least includes a femtosecond laser (1), an electrically controlled liquid crystal wave plate (2), a polarization beam splitting prism (3), Stokes optical path part, second fiber coupling mirror (7), pumping optical path part, mirror (8), pumping optical path, tapered fiber combiner (11) and signal acquisition and processing system (15), Stokes light pulses and pump light pulses output by the tapered fiber beam combiner with time overlap, space overlap, and the same linear chirp are incident on the sample to be measured on the three-dimensional electronically controlled stage, so as to Stimulate the CARS signal at the focal point of the sample to be tested; the CARS signal is used as the input of the signal acquisition and processing system to realize the acquisition and processing of the CARS signal. The invention has simple structure and convenient adjustment, can realize spectral focusing, fully utilizes the spectral energy of the ultrashort pulse and improves the resolution of the Raman resonance spectrum.

Description

technical field [0001] The invention belongs to the field of optical fiber optics and resonance spectrum microscopic imaging, and relates to a CARS microscopic imaging device and method. Background technique [0002] Coherent anti-Stokes Raman scattering (CARS) microscopic imaging technology is a resonant spectral microscopic imaging technology based on the four-wave mixing effect. The pump light pulse and the Stokes When the light pulses are incident into the sample to be tested at the same time, when the energy difference of the two beams of light pulses is equal to the resonance energy level of the target chemical bond in the sample to be tested, the four-wave mixing effect occurs to generate anti-Stokes Raman scattered light, that is CARS signal. Therefore, label-free, non-contact and chemoselective microscopic imaging of the sample to be tested can be realized by detecting the spatial distribution of the CARS signal in the sample to be tested. Since the pump light pul...

AI Technical Summary

Problems solved by technology

However, the use of space beams makes the traditional CARS microscopic imaging system bulky, complicated to adjust, requiring regular maintenance by professionals, and high operating costs

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