Method for detecting hydrogen peroxide by electrochemical and fluorescent double-signal sensor based on Fe3O4@MnO2 and carbon dots

A hydrogen peroxide and electrochemical technology, which is applied in the field of analysis and detection, can solve the problems of inaccurate determination of concentration, etc., and achieve the effects of simple and fast detection process, sensitive detection, and simple and fast preparation

Active Publication Date: 2020-02-07
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, fluorescence detection technology can only be used as a semi-quantitative

Method used

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  • Method for detecting hydrogen peroxide by electrochemical and fluorescent double-signal sensor based on Fe3O4@MnO2 and carbon dots
  • Method for detecting hydrogen peroxide by electrochemical and fluorescent double-signal sensor based on Fe3O4@MnO2 and carbon dots
  • Method for detecting hydrogen peroxide by electrochemical and fluorescent double-signal sensor based on Fe3O4@MnO2 and carbon dots

Examples

Experimental program
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Effect test

Embodiment 1

[0040] a) Fe 3 o 4 @MnO 2 Synthesis of: 0.42M Fe in 50 μL3 o 4 The solution was dispersed in 14mL water, and 3mgKMnO was added 4 Ultrasound to mix it evenly, add CTAB while stirring to emulsify the solution, then add 1.5mL MES buffer, react for 6h, to obtain a brown opaque liquid, magnetic separation to obtain a brown product that is Fe 3 o 4 @MnO 2 , washed 3 times with water, dried for later use.

[0041] b) Synthesis of carbon dots (CDs): Add 1 g of citric acid and 350 μL of diethylenetriamine to 10 mL of water, mix them evenly by ultrasonication, pour the liquid into a hydrothermal reaction kettle, raise the temperature to 200 ° C, react for 5 h, and then cool to After vacuum drying at room temperature, a brown crystalline solid, CDs, was obtained, which was ground into a powder with a mortar for later use.

[0042] c) Construction and conditions of the electrochemical sensor: at room temperature, mix the glassy carbon electrode with 2mL of the solution to be tested...

Embodiment 2

[0045] a) Fe 3 o 4 @MnO 2 Synthesis of: 0.42M Fe in 50 μL 3 o 4 The solution was dispersed in 14mL water, and 4mg KMnO was added 4 Ultrasound to mix it evenly, add CTAB while stirring to emulsify the solution, then add 2mL MES buffer, react for 7h, to obtain a brown opaque liquid, magnetic separation to obtain a brown product is Fe 3 o 4 @MnO 2 , washed 3 times with water, dried for later use.

[0046] b) Synthesis of carbon dots (CDs): Add 1 g of citric acid and 350 μL of diethylenetriamine to 10 mL of water, mix them evenly by ultrasonication, pour the liquid into a hydrothermal reaction kettle, raise the temperature to 220 ° C, and cool to After vacuum drying at room temperature, a brown crystalline solid, CDs, was obtained, which was ground into a powder with a mortar for later use.

[0047] c) The construction and conditions of the electrochemical sensor: at room temperature, the glassy carbon electrode was mixed with 2mL of the solution to be tested (containing 0...

Embodiment 3

[0050] a) Fe 3 o 4 @MnO 2 Synthesis of: 0.42M Fe in 50 μL 3 o 4 The solution was dispersed in 14mL water, and 5mgKMnO was added 4 Ultrasound to mix it evenly, add CTAB while stirring to emulsify the solution, then add 2mL MES buffer, react for 6h, to obtain a brown opaque liquid, magnetic separation to obtain a brown product is Fe 3 o 4 @MnO 2 , washed 3 times with water, dried for later use.

[0051] b) Synthesis of carbon dots (CDs): add 1 g of citric acid and 350 μL of diethylenetriamine to 10 mL of water, mix them evenly by ultrasonication, pour the liquid into a hydrothermal reaction kettle, heat up to 200 ° C, and cool to After vacuum drying at room temperature, a brown crystalline solid, CDs, was obtained, which was ground into a powder with a mortar for later use.

[0052] c) The construction and conditions of the electrochemical sensor: at room temperature, the glassy carbon electrode was mixed with 2mL of the solution to be tested (containing 0.1MKCl, 1mMFc, ...

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Abstract

The invention discloses a method for detecting hydrogen peroxide by an electrochemical and fluorescent double-signal sensor based on Fe3O4@MnO2 and carbon dots. According to the invention, the good magnetism and electrochemical properties of a composite magnetic nano material Fe3O4@MnO2 are combined with fluorescence characteristics of a fluorescent nano material carbon dot off-on to construct anelectrochemical and fluorescent double-signal sensor based on a nano material, and the electrochemical and fluorescent double-signal sensor is applied to qualitative and quantitative detection of H2O2. The method of the double-signal sensor improves the sensitivity and selectivity of a single-signal sensor, can provide accurate and diverse analysis results, is simple and convenient to operate andhigh in universality, and can be applied to accurate detection of H2O2 in a biological sample.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, in particular to 3 o 4 @MnO 2 and carbon dots for the detection of hydrogen peroxide as an electrochemical and fluorescent dual-signal sensor. Background technique [0002] Reactive oxygen species (ROS) are an important class of signaling molecules in cells, among which hydrogen peroxide (H 2 o 2 ) because of its lipophilicity, it can easily penetrate the cell membrane and enter other cells. This characteristic makes it have the function of a signaling molecule and is a typical representative of ROS in the cell environment. h 2 o 2 It mainly regulates physiological functions such as DNA damage, protein synthesis, and apoptosis. In addition, studies have found that H 2 o 2 Also involved in the process of wound healing. However, the high concentration of H 2 o 2 Cell damage can be induced, causing a variety of pathological consequences, including neurodegenerative diseases...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N21/64G01N1/28
CPCG01N1/28G01N21/6428G01N21/6486G01N27/308G01N27/3275G01N27/3277G01N27/48G01N2021/6432
Inventor 朱婉莹周颖陶梦丹沈李宓妮李新睿刘妍周学敏
Owner NANJING MEDICAL UNIV
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