a kind of ga 3+ /pda targeted synergistic antibacterial nanomaterials, their preparation and application
A composite material and polymerization reaction technology, applied in the field of antibacterial materials, can solve the problems of untargetable release, no targeting, and uncontrollable subcutaneous and in vivo infection, so as to improve the killing effect and reduce the effect of toxicity
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[0041] A kind of polydopamine composite material (also expressed as Ga in the present invention) of chelated gallium ion provided by the invention 3+ / PDA nanocomposite) preparation method, gallium ions are chelated to dopamine under acidic conditions, then induce dopamine self-polymerization to form polydopamine nanoparticles chelating gallium ions, specifically comprising the following steps:
[0042] (1) Mix the aqueous solution of dopamine or dopamine salt with the aqueous solution of gallium salt, and adjust the pH of the mixed system to 2-4, so that the gallium salt and dopamine in the mixed system undergo a chelation reaction, and obtain the chelated mixed system;
[0043] (2) adding an oxidizing agent to the mixed system after chelation, so that the dopamine in the mixed system undergoes a polymerization reaction to obtain a mixed system after dopamine polymerization;
[0044] (3) Add a precipitating agent to the mixed system after dopamine polymerization, so that the...
Embodiment 1
[0074] Ga 3+ / PDA nanoparticles preparation:
[0075] Dissolve dopamine hydrochloride and gallium nitrate in a certain volume of deionized water at a molar ratio of 1:1-3, stir magnetically for about 30 minutes, and then add 50-500 microliters of 30% hydrogen peroxide solution, at pH=3, 15- Magnetic stirring was carried out at 40°C for 12-48 hours. After the reaction, add twice the volume of absolute ethanol, centrifuge at 14,000g for 10-30min, and wash the precipitate with 50mM Tris-HCl (pH=8.5) three times, then wash twice with deionized water and dry to constant weight You can get the finished product.
[0076] figure 1 a is H 2 o 2 Induced self-aggregation of dopamine to produce Ga 3+ / PDA nanoparticles (Ga 3+ / PDA) schematic diagram; figure 1 b is Ga 3+ Schematic diagram of the pH-responsive release. When Ga 3+ When / PDA is in a normal weakly alkaline physiological environment, Ga 3+ Tightly chelated with dopamine, Ga 3+ not easily from Ga 3+ / PDA nanopartic...
Embodiment 2
[0081] Ga 3+ / Characteristics of the surface charge of PDA nanoparticles changing with pH
[0082] Determination of Ga using a particle size analyzer 3+ / PDA nanoparticles surface potential, by Figure 5 It can be seen that its surface potential has the characteristics of pH-responsive change: at pH=7.4, its surface potential value is -7.43±0.74mV, and at pH=6.5, its surface potential value reverses to 13.67±1.49mV. When it was further reduced to 5.5, the surface potential value further increased to 21.49±2.76mV. This is due to the deprotonation of catechol groups in weak alkaline, which makes the nanoparticles negatively charged, and when the pH is reduced to 6.5 and 5.5, the catechol and amine groups are protonated, making the nanoparticles negatively charged. Positively charged. The results showed that the prepared Ga 3+ / PDA surface potential can realize the reversal of surface potential in the range of pH 7.4-6.5, which can ensure the obvious Ga from pH 7.4 to 6.5 3...
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