Ga<3+>/PDA targeted co-antibacterial nanometer material, and preparation and application thereof
A composite material and polymerization technology, applied in the field of antibacterial materials, can solve the problems of untargeted release, no targeting, uncontrollable subcutaneous and in vivo infection, etc., to achieve the effect of improving the killing effect and reducing the toxic effect
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[0041] The invention provides a polydopamine composite material chelating gallium ions (also expressed as Ga in the invention) 3+ / PDA nanocomposite material), in which gallium ions are chelated to dopamine under acidic conditions, and then dopamine is induced to self-polymerize to form gallium ion-chelated polydopamine nanoparticles, which specifically includes the following steps:
[0042] (1) Mix the aqueous solution of dopamine or dopamine salt with the aqueous solution of gallium salt, and adjust the pH of the mixed system to 2-4, so that the gallium salt and dopamine in the mixed system undergo a chelation reaction to obtain a chelated mixture system;
[0043] (2) Adding an oxidizing agent to the chelated mixed system to cause the dopamine in the mixed system to undergo a polymerization reaction to obtain a mixed system after dopamine polymerization;
[0044] (3) Add a precipitating agent to the mixed system after dopamine polymerization, so that the polydopamine complex of che...
Embodiment 1
[0074] Ga 3+ / PDA Nanoparticle Preparation:
[0075] Dissolve dopamine hydrochloride and gallium nitrate in a certain volume of deionized water at a molar ratio of 1:1-3. After magnetic stirring for about 30 minutes, add 50-500 microliters of 30% hydrogen peroxide solution at pH=3,15- The reaction was stirred magnetically at 40°C for 12-48h. After the reaction, add double volume of absolute ethanol, centrifuge for 10-30min under 14000g centrifugal force, and wash the precipitate with 50mM Tris-HCl (pH=8.5) three times, then wash with deionized water twice and then dry to constant weight You can get the finished product.
[0076] figure 1 a is H 2 O 2 Inducing dopamine self-polymerization to prepare Ga 3+ / PDA Nanoparticles (Ga 3+ / PDA) schematic diagram; figure 1 b is Ga 3+ Schematic diagram of pH-responsive release. When Ga 3+ / PDA is in a normal weak alkaline physiological environment, Ga 3+ Chelate tightly with dopamine, Ga 3+ Not easy from Ga 3+ / PDA nanoparticles are dissoci...
Embodiment 2
[0081] Ga 3+ / PDA Nanoparticle Surface Charge Variation Characteristics with pH
[0082] Determination of Ga using particle size analyzer 3+ / PDA nanoparticle surface potential, by Figure 5 It can be seen that its surface potential has the characteristics of pH responsive change: when pH=7.4, its surface potential value is -7.43±0.74mV, and when pH=6.5, its surface potential value is inverted, which is 13.67±1.49mV. When it is further reduced to 5.5, its surface potential value further increases, reaching 21.49±2.76mV. This is due to the deprotonation of the catechol groups when weakly alkaline, making the nanoparticles negatively charged, and when the pH drops to 6.5 and 5.5, the catechol and amine groups are protonated, making the nanoparticles Positively charged. The results show that the prepared Ga 3+ The surface potential of / PDA can be reversed in the range of pH 7.4-6.5, and it can ensure that obvious Ga is present at pH from 7.4 to 6.5 3+ Chelation and dissociation tra...
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