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Preparation method of tissue engineering skin

A technology of tissue engineering skin and skin cells, which is applied in the field of tissue engineering skin preparation, can solve the problems of uncontrolled recovery of scars, inability to treat wounds with drugs, and easy infection, etc., to reduce human body rejection, excellent air permeability and Water absorption, easy absorption effect

Inactive Publication Date: 2020-02-28
黑龙江紫泰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Skin is the largest tissue and organ of the human body. It is a non-specific and immune barrier of the human body. Large-scale skin defects can cause serious health problems and even threaten life. However, repairing large-area skin loss has always been a medical problem. Great physical and psychological pain, and easy to cause infection, slow recovery, recovery scars cannot be controlled, and effective drug treatment cannot be performed on the trauma surface

Method used

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  • Preparation method of tissue engineering skin
  • Preparation method of tissue engineering skin
  • Preparation method of tissue engineering skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Preparation method of tissue engineered skin.

[0033] The preparation method of the tissue-engineered skin described in this example is to use skin cells as seed cells for culture and expansion, and use nano-electrospinning material as a support, and print the cultured and expanded cells on the support by 3D printing technology. To obtain tissue-engineered skin, the specific steps are as follows:

[0034] (1) Preparation of nano-electrospinning materials

[0035] Using DMF (N,N-dimethylformamide) as solvent, prepare a PVP spinning solution with a mass fraction of 16%, in a spinning bottle at 30°C, swell for 24 hours to obtain a spinning solution; On the electrospinning machine, under the voltage of 15kV, the distance between the capillary and the collection screen is 30cm, and the nano electrospinning material with a thickness of 0.5mm is obtained after processing for 2 minutes;

[0036] (2) Culture of human skin cells

[0037] Take the skin tissue and...

Embodiment 2

[0040] Example 2. Preparation method of tissue engineered skin.

[0041] The preparation method of the tissue-engineered skin described in this example is to use skin cells as seed cells for culture and expansion, and use nano-electrospinning material as a support, and print the cultured and expanded cells on the support by 3D printing technology. To obtain tissue-engineered skin, the specific steps are as follows:

[0042] (1) Preparation of nano-electrospinning materials

[0043] Using DMF as a solvent, prepare a PVP spinning solution with a mass fraction of 10%, swell it in a spinning bottle at 29°C for 26 hours, and obtain a spinning solution; put the spinning solution on an electrospinning machine, under a voltage of 16kV, capillary The distance between the collection screen and the collection screen is 25cm, and the nano-electrospinning material with a thickness of 0.45mm is obtained after processing for 2 minutes;

[0044] (2) Culture of human skin cells

[0045] Take ...

Embodiment 3

[0048] Example 3. Preparation method of tissue engineered skin.

[0049] The preparation method of the tissue-engineered skin described in this example is to use skin cells as seed cells for culture and expansion, and use nano-electrospinning material as a support, and print the cultured and expanded cells on the support by 3D printing technology. To obtain tissue-engineered skin, the specific steps are as follows:

[0050] (1) Preparation of nano-electrospinning materials

[0051] Using DMF as a solvent, prepare a PVP spinning solution with a mass fraction of 14%, swell it in a spinning bottle at 31°C for 22 hours, and obtain a spinning solution; put the spinning solution on an electrospinning machine, under 18kV voltage, capillary The distance between the collection screen and the collection screen is 28cm, and the nano-electrospinning material with a thickness of 0.55mm is obtained after processing for 2 minutes;

[0052] (2) Culture of human skin cells

[0053] Take the...

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Abstract

The invention discloses a preparation method of tissue engineering skin, belongs to the technical field of tissue engineering and aims to solve the problem that defects exist in current making methodsof tissue engineering skin support materials and interaction mode of cells and the support materials. The preparation method includes following steps: (1), preparing a nano electrostatic spinning material; (2), culturing human body skin cells; (3), culturing the cells onto a nanofiber material. The preparation method is simple in building method and few in operation step, and good animal in-vivoexperiment effect can be acquired.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a preparation method of tissue engineering skin. Background technique [0002] Skin is the largest tissue and organ of the human body. It is a non-specific and immune barrier of the human body. Large-scale skin defects can cause serious health problems and even threaten life. However, repairing large-area skin loss has always been a medical problem. Great physical and psychological pain, and it is easy to cause infection, slow recovery, recovery scars cannot be controlled, and effective drug treatment cannot be performed on the trauma surface. People are always looking for ideal skin substitutes. [0003] Skin tissue engineering is to use the principles of biology and engineering to construct tissue substitutes for repairing, maintaining and improving the function of damaged tissues. The core is to create a three-dimensional growth scaffold, and to culture...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/54A61L27/60D01D5/00B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/60D01D5/003A61L27/3886A61L27/3687A61L27/54D01D5/0076B33Y10/00B33Y70/00B33Y80/00A61L2300/406A61L2300/426A61L2300/412
Inventor 陶冶康晓菲
Owner 黑龙江紫泰科技有限公司
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