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Method for separating and extracting D-lactic acid from D-sodium lactate fermentation broth

A fermented liquid and sodium lactate technology, which is applied in the direction of microorganism-based methods, separation/purification of carboxylic acid compounds, fermentation, etc., can solve the problems of many unit operations, long process routes, and low product quality, and achieve low operating energy consumption, No waste liquid pollution, high product quality effect

Inactive Publication Date: 2020-03-06
SINOPEC YANGZI PETROCHEM +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects of long process route, many unit operations, serious waste liquid pollution, high energy consumption, low recovery rate and low product quality in the prior art for lactic acid extraction, the present invention provides a Method for separating and extracting D-lactic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of D-sodium lactate fermented liquid comprises the following steps that are connected in sequence:

[0039] (1) Plate culture: Inoculate Bacillus BS1-5 into plate medium for anaerobic culture, culture temperature 30°C, culture time 48h;

[0040] (2) Seed cultivation: inoculate the bacillus grown on the plate in step (1) into the seed medium for anaerobic cultivation, the cultivation temperature is 30°C, and the cultivation time is 24h;

[0041] (3) fermentation acid production: the seed culture liquid obtained in step (2) is inoculated into the fermentation medium for fermentation acid production, the inoculum size is 15%, the fermentation temperature is 30 ° C, and nitrogen is introduced to maintain its anaerobic environment, using The neutralizer controls the pH of the fermentation system at 6.0.

[0042] The components of the plate medium are (g / L): glucose 10, yeast extract 1, anhydrous sodium acetate 1, anhydrous magnesium sulfate 0.1, potas...

Embodiment 2

[0047] (1) Plate culture: Inoculate Bacillus BS1-5 into plate medium for anaerobic culture, culture temperature 45℃, culture time 20h;

[0048] (2) Seed cultivation: inoculate the bacillus grown on the plate in step (1) into the seed medium for anaerobic cultivation, the cultivation temperature is 45°C, and the cultivation time is 12h;

[0049] (3) fermentation acid production: the seed culture liquid obtained in step (2) is inoculated into the fermentation medium for fermentation acid production, the inoculum size is 3%, the fermentation temperature is 45 ° C, and nitrogen is introduced to maintain its anaerobic environment, using The neutralizer controls the pH of the fermentation system at 7.0.

[0050] The components of the plate medium are (g / L): glucose 30, yeast extract 3, anhydrous sodium acetate 4, anhydrous magnesium sulfate 0.4, potassium dihydrogen phosphate 3, agar 25.

[0051] The components of the seed medium are (g / L): glucose 40, yeast extract 3, peptone 3, a...

Embodiment 3

[0055] (1) Plate culture: Inoculate Bacillus BS1-5 into plate medium for anaerobic culture, culture temperature 37°C, culture time 24h;

[0056] (2) Seed cultivation: inoculate the bacillus grown on the plate in step (1) into the seed medium for anaerobic cultivation, the cultivation temperature is 37°C, and the cultivation time is 20h;

[0057] (3) fermentation acid production: the seed culture liquid obtained in step (2) is inoculated into the fermentation medium for fermentation acid production, the inoculum size is 10%, the fermentation temperature is 37 ° C, and nitrogen is introduced to maintain its anaerobic environment, using The neutralizer controls the pH of the fermentation system at 6.5.

[0058] The components of the plate medium are (g / L): glucose 20, yeast extract 2, anhydrous sodium acetate 2, anhydrous magnesium sulfate 0.3, potassium dihydrogen phosphate 2, agar 20.

[0059] The components of the seed medium are (g / L): glucose 20, yeast extract 2, peptone 2,...

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Abstract

The invention discloses a method for separating and extracting D-lactic acid from D-sodium lactate fermentation broth. The method includes the following steps in order: (1) performing microfiltrationon D-sodium lactate fermentation broth so as to obtain microfiltration fermentation broth; (2) performing ultra-filtration on the microfiltration fermentation broth obtained in the step (1) so as to obtain ultra-filtration fermentation broth; (3) performing nanofiltration on the ultra-filtration fermentation broth obtained in the step (2) so as to obtain nanofiltration fermentation broth; (4) adding a hydrogen peroxide solution into the nanofiltration fermentation broth obtained in the step (3) to perform decolorization, so as to obtain a decolorized solution; (5) performing electrodialytic treatment on the decolorized solution obtained in the step (4), so as to obtain a lactic acid clear solution; and (6) concentrating the lactic acid clear solution obtained in the step (5) so as to obtain a D-lactic acid product. The method is simple, easy in operation, low in energy consumption, high in recovery rate and high in product quality and has no waste liquid pollution.

Description

technical field [0001] The invention relates to a method for separating and extracting D-lactic acid from D-sodium lactate fermentation liquid, and belongs to the field of D-lactic acid extraction. Background technique [0002] Lactic acid is one of the three major organic acids in the world, and it can be widely used in food, medicine, chemical industry and agriculture. Special D-lactic acid can be widely used in pharmaceutical, chemical and agricultural fields. Polylactic acid synthesized from D-lactic acid has become the most promising green material in the 21st century due to its good biodegradability and other excellent use characteristics (such as transparency, thermoplasticity, product safety, etc.). In addition, herbicides such as bio-pesticides "Puma" and "Weiba" made of high-purity D-lactic acid, as new low-toxicity pesticides, can effectively kill weeds and insects, both economically and reliably. On the market, the price of D-lactic acid of the same grade is 5-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C59/08C12P7/56C12R1/01
CPCC07C51/42C12P7/56C07C59/08Y02P20/59
Inventor 方晓江刘经伟李泽壮杨爱武
Owner SINOPEC YANGZI PETROCHEM
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