High-flux ultra-sensitive double-label time-resolved immunochromatographic test strip and application thereof

A time-resolved fluorescence, test strip technology, applied in analytical materials, biological material analysis, measurement devices, etc., can solve the problem that the sensitivity is difficult to meet people's requirements, and achieve the effects of rapid detection, high sensitivity and good stability
CN110873794AInactive Publication Date: 2020-03-10CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2020-03-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a high-flux ultra-sensitive double-labeled time-resolved immunochromatographic test strip and application thereof. The high-flux ultra-sensitive double-label time-resolved immunochromatographic test strip comprises a sample pad, a nitrocellulose film, a water absorption pad and a PVC bottom plate, wherein two detection lines and a quality control line are arranged on the nitrocellulose film; the detection line is respectively coated with antigens of a small molecular compound A and a small molecular compound B; and the quality control line is coated with a goat anti-chicken antibody. The invention also discloses a complete set of time-resolved fluorescent microsphere coupled antibody probe, which consists of a time-resolved fluorescent microsphere labeled anti-smallmolecule compound A antibody, a time-resolved fluorescent microsphere labeled anti-small molecule compound B antibody and a time-resolved fluorescent microsphere labeled chicken IgY antibody. The test strip and the complete set of time-resolved fluorescent microsphere coupled antibody probe can be used for rapidly, sensitively and accurately detecting various small molecular harmful compounds.
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Description

technical field

[0001] The invention belongs to the detection of small molecule harmful compounds in the field of food safety, and in particular relates to a high-throughput ultrasensitive double-labeled time-resolved immunochromatographic test strip and an application thereof. Background technique

[0002] Immunochromatography technology is an immunoassay technology that has rapidly emerged in recent years by integrating the advantages of immunology technology and chromatography technology. This method has been widely used in the detection of small molecules. The nitrocellulose membrane material is used as the solid phase, and the sample to be tested is used as the mobile phase. Through capillary chromatography, the analyte reacts specifically with the receptors on the membrane for the analyte. The analyte reacts with the detection line on the NC membrane, and the detection result is determined by detecting or visually observing the color of the marker. In addition to the...

Claims

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