Solid concentrated growth factor as well as preparation method and application thereof

Abstract

The invention discloses a solid concentrated growth factor as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out freeze drying orvacuum drying on an extracted gelatinous concentrated growth factor to obtain the solid concentrated growth factor. The solid concentrated growth factor prepared by the invention contains a large amount of fibrin with a three-dimensional matrix network structure, and is of a porous structure; compared with a gelatinous concentrated growth factor, the advantages are that the storage period is longer, the biomechanical strength is remarkably better, meanwhile, the capability in promoting BMSCs proliferation, differentiation and mineralization is better, the solid concentrated growth factor can be independently taken as an implant material for bone defect repair, and the biomechanical strength of repaired injured vertebrae is also remarkably improved; the preparation method of the solid concentrated growth factor is simple to operate, venous blood can be used as a raw material, and the prepared material is better in performance and wider in application range, and a promising clinical application prospect and potential economic value are achieved.

Description

technical field [0001] The invention belongs to the field of medical biology, and in particular relates to a solid-state concentrated growth factor and its preparation method and application. Background technique [0002] Osteoporotic vertebral fracture (Osteoporotic vertebral fracture, OVF) is one of the serious complications of osteoporosis (Osteoporosis, OP), which is a difficult clinical treatment. After OVF occurs, compression and intercalation changes occur, and the bone defect in the vertebral body is left behind by positional reduction or manual reduction, which is prone to poor fracture healing and poor recovery of the mechanical strength of the vertebral body, resulting in unrelieved pain and progressive collapse of the vertebral body. Therefore, conservative treatment such as simple analgesia, bed rest, bracing and manual reduction can no longer fully meet the needs of OVF treatment in clinic. Promoting the early healing of the fractured vertebral body, maintaini...

AI Technical Summary

Problems solved by technology

[0003] Polymethylmethacrylate (PMMA) is currently one of the most commonly used implant materials for the treatment of OVF. Although it can provide sufficient support strength and cementation, it also has some obvious disadvantages: it cannot be degraded in the body. Absorption, poor biocompatibility, no biological activity, excessive elastic modulus, release of a large amount of heat during polymerization, toxic unpolymerized monomers, etc., have led to some serious complications in clinical application

[0006] At present, domestic and foreign reports have taken blood from the veins of goats, beagle dogs, rabbits, and Vista rats to prepare CGF after centrifugation and then conducted experiments. The cost is high and blood collection is difficult, and it is not easy to master; ℃ refrigerator for 0 to 48 hours, so it is generally necessary to use it in cash

In addition, in orthopedics, gel-like CGF is usually prepared and then cut into small pieces and then mixed with autologous bone or allogeneic bone and implanted in the bone defect. The effect of implantation on bone defect repair is unclear

In addition, CGF gel used alone has problems such as poor mechanical properties and inability to store for a long time, which seriously limits its clinical application.

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