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A plant fermented composition with the function of whitening and lightening spots

A technology of plant fermentation and composition, which is applied in the direction of drug combination, medical preparations containing active ingredients, skin care preparations, etc., and can solve the problems of little research

Active Publication Date: 2021-01-29
魏珂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, some people have begun to improve the function of plants through biotransformation (fermentation), but there is still little research

Method used

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  • A plant fermented composition with the function of whitening and lightening spots
  • A plant fermented composition with the function of whitening and lightening spots
  • A plant fermented composition with the function of whitening and lightening spots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A fermented plant composition with the function of whitening and lightening spots, characterized in that:

[0036] Seabuckthorn fruit oil, aloe vera extract, salvia miltiorrhiza extract, and Morinda officinalis sugar chain solution are used as substrates, and Lactobacillus gasseri ATCC19992 and Citrobacter yellow CGMCC.No 2998 are used for fermentation to obtain.

[0037] The concrete steps of above-mentioned fermentation are:

[0038] (1) Configure culture medium:

[0039] According to the parts by weight, mix 5 parts of aloe extract, 10 parts of Danshen extract, and 2 parts of Morinda officinalis sugar chain solution, then add 2 parts of seabuckthorn fruit oil, 2 parts of glycerin and appropriate amount of Tween 80, heat and stir to emulsify evenly Afterwards, ultraviolet sterilized for standby;

[0040] (2) Aerobic fermentation of yellow citric acid bacteria:

[0041] Activation of citric acid bacteria CGMCC.No 2998: Take a ring of citric acid bacterium CGMCC.No 2...

Embodiment 2

[0048] Cytotoxicity Test:

[0049] Experimental grouping: the fermented composition filtered in Example 1 was dissolved in serum-free DMEM culture fluid, and 5 samples were prepared with mass fractions of 0.05%, 0.10%, 0.25%, 0.50%, and 1.00%, respectively, and cultured in serum-free DMEM The solution was the negative control sample group, and each sample was set up with 3 replicates.

[0050] MTT assay: Adjust the concentration of well-grown L929 cells to 1×10 5 cells / mL, inoculated into 96-well plates at 100 μL / well, and cultured in serum-free DMEM medium for 24 hours at 37° C. in a 5% CO2 environment. After adherent growth, the culture medium was discarded, and 100 μL of sample solutions with different mass fractions were added to each well according to the experimental grouping scheme. The negative control group continued to be cultured in serum-free DMEM for 48 hours. Add 20 μL of 5 mg / mL MTT solution to each well of the cell culture plate, and incubate in an incubator ...

Embodiment 4

[0070] In vitro tyrosinase inhibition assay:

[0071] Solution preparation: preparation containing 0.025mol / L Na 2 HPO 4 and 0.02mol / L KH 2 PO 4 PBS solution (pH=6.8); prepare 1 mg / ml tyrosine solution and 200 U / ml tyrosinase solution with PBS.

[0072] Example 1 and Comparative Examples 1-3 were used as treatment groups, and vitamin C ethyl ether solution was used as a positive control group. PBS preparation vitamin C ethyl ether solution, mass fraction is respectively 0.05%, 0.10%, 0.25%, 0.50%, 1.00%; PBS preparation embodiment 1 group, comparative example 1-3 group solution, mass fraction is respectively 0.05%, 0.10%, 0.25%, 0.50%, 1.00%.

[0073] Enzyme activity detection: use a micropipette to pipette the four groups of samples a, b, c, and d according to Table 3 and place them in 1.5ml EP tubes (prepared reaction system is 1000ul), in a 35°C water bath for 10min; add tyrosinase solution 200ul, 35°C water bath for 30min, 150ul from each reaction tube was added to a...

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Abstract

The invention discloses a plant fermented composition which is applied to cosmetics and has the function of whitening and blemishes. Obtained by fermentation of Lactobacillus gasseri ATCC19992 and Citrobacter yellow CGMCC.No 2998. In recent years, through a lot of research, it has been found that fermenting plants with specific strains can significantly improve their cosmetic efficacy. After years of research and practice, the R&D team of our company has discovered a natural plant that is fermented with probiotics. Finally, it can play a very significant effect of whitening and removing freckles, and its effect of removing freckles and depigmentation is excellent, especially for chloasma and stubborn pigmentation.

Description

technical field [0001] The invention relates to a fermented plant composition with the function of whitening and lightening spots, belonging to the technical field of biological cosmetics. Background technique [0002] Human skin color is affected by heredity and environment, and the most important factor is the content of melanin in epidermal tissue. Melanocytes synthesize melanin through a series of biochemical processes, and tyrosinase plays an important role in the formation of melanin. Reducing melanin production by inhibiting tyrosinase activity is a research hotspot in skin science. The mechanism of UV-induced melanin production is that UV-induced free radicals change the metabolic process of cells, so scavenging free radicals is also one of the methods to inhibit melanin production. [0003] The pigmentation process of human skin is very important. It not only determines the color of skin, eyes and hair, but also is the first barrier to protect the skin from the dam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9794A61K8/9789A61K8/92A61K8/60A61P17/00A61Q19/00A61Q19/02
CPCA61K8/60A61K8/922A61K2800/85A61Q19/00A61Q19/02A61K8/9789A61K8/9794A61P17/00
Inventor 魏珂
Owner 魏珂
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