A kind of preparation method of microcapsules carrying probiotics and products and applications thereof

Abstract

The invention provides a preparation method of microcapsules carrying probiotics, which is characterized in that the method comprises the following steps: (1) dissolving sodium alginate in a solvent to obtain a sodium alginate solution; (2) dissolving the probiotic bacteria Mix evenly with the sodium alginate solution obtained in step (1) to obtain a probiotic bacteria suspension; (3) spray a salt solution into the probiotic bacteria suspension obtained in step (2), stir and solidify, and obtain the probiotic bacteria-loaded Microcapsules. The invention adopts sodium alginate as the wall material of the microcapsules, and the spraying method combined with the ion curing method to prepare the microcapsules. The prepared microcapsules have good spherical shape, small particle size, good dispersibility and strong pH sensitivity. The surface of the capsule is denser, which can effectively protect probiotics and meet the needs of subsequent in vivo evaluation of animals.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of microcapsules carrying probiotics, products prepared therefrom and applications. Background technique [0002] Probiotics are active microorganisms that can improve the host's intestinal microecological balance and are beneficial to the host. They colonize the human intestine and reproductive system and can produce definite health effects. When a certain amount of probiotics are ingested by the human body, it will have many health-promoting effects, including controlling intestinal infections, enhancing immunity, regulating plasma and cholesterol levels, and anti-cancer. For patients with lactose intolerance, it can promote their utilization of lactose. In recent years, more and more studies have confirmed that probiotics can inhibit the growth of pathogenic bacteria, eliminate carcinogenic factors, maintain normal intestinal flora, and...

AI Technical Summary

Problems solved by technology

[0004] CN104911171A discloses a method for preparing probiotic microcapsules with sodium alginate and gelatin composite attapulgite. The method firstly purifies attapulgite, then mixes attapulgite with sodium alginate and gelatin to form a composite wall material, and then mixes the prepared attapulgite The probiotic bacteria suspension is mixed evenly with the above-mentioned composite wall material under certain conditions, and then slowly dripped into CaCl 2 Medium solidification and molding, and finally vacuum freeze drying to obtain probiotic microcapsules. The probiotic microcapsule embedding material prepared by this method has low activity and low survival rate

[0005] CN102228235A discloses a method for preparing probiotic microcapsules and its application. The sodium alginate solution and the modified starch solution are mixed in equal volumes, and then mixed with probiotics, and CaCl is sprayed through the sprayer nozzle. 2 solution, standing and solidifying, washing and filtering with physiological saline, although this method enhances the mechanical strength of the capsule wall to a certain extent, but the size of the obtained capsule is difficult to control, it is difficult to apply to industrial production in the middle of food and medicine, and adding denaturation After being starched, the pH-sensitive release characteristics of the microcapsules are weakened, so that after they are immersed in the stomach, some microcapsules cannot become dense on the surface in the acidic environment of the stomach, and cannot disintegrate quickly in the weak acid environment of the intestinal tract , releasing the entrapped probiotics

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