Living body nerve scaffold with central perfusion system and manufacturing method thereof
A nerve and living technology, applied in the field of the manufacture of living nerve scaffolds, can solve the problems of insufficient local blood supply, easy formation of glial scars, etc., and achieve the effects of reducing concentration, promoting proliferation and differentiation, and high cell density.
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Embodiment 1
[0050] This embodiment provides a living nerve support, such as figure 1 As shown, it includes nerve bundle 1 and endothelial cell layer 2. There is a perfusion channel 3 inside the nerve bundle, and the endothelial cell layer 2 is modified.
[0051] According to the phenomenon that nerve cells grow linearly into a bundle structure on a surface with a large curvature, the living nerve scaffold is formed by linearly growing neural stem cells SH-SY5Y in a hydrogel catheter.
Embodiment 2
[0053] This embodiment provides a mold for manufacturing the living nerve support according to the first aspect of the present invention, including: a first positioning plate 4 , a lower mounting plate 5 , an upper mounting plate 6 and a needle 9 .
[0054]There are two first positioning plates 4 , which are respectively provided with a certain number of first holes for installing the needles 9 .
[0055] The lower mounting plate 5 and the upper mounting plate 6 can be assembled together to form a certain number of lumens, and surfaces capable of cooperating with the first positioning plate are formed at both ends of the lumens. Thus, when the lower mounting plate 5 and the upper mounting plate 6 are placed between the two first positioning plates 4, the first hole is aligned with the axis of the lumen.
[0056] In a preferred embodiment, the lower mounting plate 5 has a first surface parallel to the lumen, and a certain number of first grooves are arranged on the first surfac...
Embodiment 3
[0063] This embodiment provides a method for manufacturing a living nerve scaffold as described in Embodiment 1 using the mold in Embodiment 2, and the specific implementation method is as follows.
[0064] First, a hydrogel conduit was fabricated to provide a three-dimensional linearized growth environment for neural stem cell lines.
[0065] The material of the hydrogel catheter is agarose, the outer diameter is 2 mm, and the lumen diameter is 1 mm. The manufacturing method of the agarose catheter is specifically to weigh 3g of agarose powder, dissolve it in 100ml of deionized water, add a magnetic stirrer in the beaker, and heat at 100°C and 120 rpm until the agarose solution boils , the boiling agarose solution was left to stand for 1 minute until the bubbles disappeared, poured into a mold, and left to stand until the agarose solution cooled to room temperature to obtain a structurally stable agarose catheter.
[0066] The mold as figure 2 As shown, it is composed of a...
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