Cancer suppressor gene methylation high-throughput detection kit of colorectal cancer and application thereof

Abstract

The invention belongs to the technical field of biology and particularly relates to a cancer suppressor gene methylation high-throughput detection kit of the colorectal cancer and application thereof.The kit comprises methylation specific multiplex PCR amplification primers having the sequences shown as SEQ ID NO: 1-20 and label primers of which the sequences are shown as SEQ ID NO: 21-41. According to the invention, the target methylation detection areas of one or more genes can be captured at one time and the methylation mutation sequence information of one or more gene target regions of the sample is obtained, so that the methylation state of the target region can be accurately and effectively analyzed and thus the high-throughput, high-sensitivity and high-accuracy methylation detection on the target gene region becomes the reality. The method further has technical characteristics of low cost, simple and rapid operation and the like; and the sample library construction can be completed only through two rounds of PCR.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a high-throughput detection kit for colorectal cancer tumor suppressor gene methylation and its application. Background technique [0002] The incidence of colorectal cancer (CRC) ranks third among malignant tumors in the world, and it has become the third leading cause of new cancer cases and deaths. Regular screening can detect colorectal cancer early, allowing early intervention to prevent disease progression or even cure the disease. However, 60% to 70% of patients were diagnosed with advanced CRC at the time of first diagnosis. Statistics from the United States Preventive Services Task Force (USPSTF) show that if healthy people undergo routine regular inspections every year to achieve early diagnosis and treatment of CRC, about 60% of CRC patients can be prevented from dying, and the average five-year survival rate can reach From 46% to 73%. Therefore, an effective early scre...

AI Technical Summary

Problems solved by technology

[0006] Before the popularization of sequencing technology, high-performance liquid chromatography (HPLC) was a classic method for methylation determination, but it could only measure the total DNA methylation level, and could not measure specific sequences.

The classic method for specific DNA sequence determination is Southern hybridization analysis, but this method has low sensitivity and requires a large amount of samples

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