A method for open production of Trichoderma solid strain
A solid strain, open technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, immobilized on/in organic carriers, etc., can solve the problems of unreachable final products and increased content of miscellaneous bacteria, etc. Achieve the effect of improving production efficiency and reducing production cost
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Embodiment 1
[0031] Example 1 Effects of different pH value mixtures (mixtures of full-valent amino acid solution and corn stalk powder diluted in different times) on the growth of bacterial strain NJAU4742
[0032] Fully mix the full-valent amino acid solution diluted with different multiples and the corn stalk powder according to the volume / mass ratio of 2.5 / 1, let it stand for 11 hours, and adjust the initial pH value of the mixture (11 treatments are set, each treatment is repeated three times, each The corn stalk weight that handles is 5 kilograms), inoculate bacterial strain NJAU4742 fermented liquid by the inoculum amount of 10% of corn stalk powder quality, place in the shallow pan (thickness 3~4 centimetres) with hole, control space temperature 28~30 ℃, humidity 83% RH, let it ferment for 5 days. The number of spores was recorded with a hemocytometer, and the results are shown in Table 1.
[0033]The results showed (Table 1): the mixture of full-valent amino acid solution and cor...
Embodiment 2
[0045] Example 2 Effect of the mixture prepared from different kinds of stalks and full-valent amino acids on sporulation of Trichoderma strain NJAU4742
[0046] Fully mix the full-valent amino acid solution diluted 20 times with the corn / wheat / rice straw powder according to the volume / mass ratio of 2.5 / 1 (repeated 3 times for each straw powder), let stand for 18 hours, and adjust the initial pH of the mixture When the value is 1.2 to 1.4, inoculate the Trichoderma strain NJAU4742 fermentation liquid with an inoculation amount of 5% of the straw powder mass, place it in a shallow dish with holes (thickness 3 to 4 cm), control the space temperature at 28 to 30 °C, and humidity at 88 °C. %RH and let it ferment for 5 days. The number of spores was recorded with a hemocytometer, and the results are shown in Table 3.
[0047] Using four kinds of stalks (corn stalks, wheat stalks, rice stalks), stevia rebaudiana and a full-valent amino acid solution as raw materials, the solid ferm...
Embodiment 3
[0053] Example 3 Pilot production of the agricultural microbial inoculant containing Trichoderma strain NJAU4742
[0054] Fully mix the full-valent amino acid solution diluted 20 times with 110 kg of corn stalk powder according to the volume / mass ratio of 2.5 / 1, let stand for 12 hours, adjust the initial pH value of the mixture to 1.2-1.4, and adjust the pH value of the mixture according to the mass of the straw powder. 7% of the inoculum was inoculated with Trichoderma strain NJAU4742 fermented liquid, placed in a perforated shallow dish (thickness 3-4 cm), controlled space temperature 28-30 ° C, humidity 83% RH, allowed it to ferment for 5 days. After the fermentation, the spore concentration of the strain NJAU4742 is calculated by counting on a hemocytometer, and at the same time, the fermented material is spread out until the water is lost to less than 35%, which is the agricultural microbial inoculant containing the Trichoderma strain NJAU4742.
[0055] The main technical...
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