Lactobacillus plantarum for high-moisture oat silage and application thereof

A technology of Lactobacillus plantarum and high moisture, which is applied in the field of silage, can solve the problems of less lactic acid bacteria preparations and high production costs, and achieve the effects of strong acid production capacity, reduced content, and low production costs

Active Publication Date: 2020-04-10
SICHUAN ACAD OF GRASSLAND SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Adding chemical additives such as formic acid, propionic acid, and potassium sorbate can improve the fermentation quality of high-moisture silage to a certain extent.

Method used

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  • Lactobacillus plantarum for high-moisture oat silage and application thereof
  • Lactobacillus plantarum for high-moisture oat silage and application thereof
  • Lactobacillus plantarum for high-moisture oat silage and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] This embodiment is an embodiment of strain screening, determination and preservation.

[0036] A strain of Lactobacillus plantarum used for high-moisture oat silage was isolated and screened multiple times from samples such as pickles, silage, livestock manure, gastric juice, and yogurt on the Qinghai-Tibet Plateau. The Lactobacillus plantarum was preserved in China on May 15, 2019 The General Microbiology Center of the Microbial Culture Collection Management Committee, the Lactobacillus plantarum strain is Lactobacillus plantarum (L. plantarum), and the preservation number is CGMCC No.17740.

Embodiment 3

[0045] This embodiment is an application example of the plant lactic acid bacteria obtained in Example 1 in the fermentation of high-moisture oat silage, and the specific method steps are as follows:

[0046] 1) Raw material preparation: Aba Hongyuan County flowering oats in September, manually harvested and chopped to 5-8cm;

[0047] 2) Strain activation: Inoculate the Lactobacillus plantarum preserved in the ampoule into the MRS liquid medium, culture at 30°C for 12-14 hours, subculture twice to obtain the activated strain, and inoculate the activated strain into the MRS liquid medium Expand the culture, use cyclodextrin to wrap the bacteria, freeze-dry, make a solid bacteria preparation, and make the number of viable bacteria >109cfu / g;

[0048] 3) Preparation and fermentation of silage raw materials: Dilute the solid bacterial preparation with distilled water, inoculate it into the fresh grass raw material according to the inoculum amount of 105 cfu / g of viable bacteria, m...

Embodiment 4

[0052] This embodiment is an application example of the plant lactic acid bacteria obtained in Example 1 in the fermentation of high-moisture oat silage, and the specific method steps are as follows:

[0053] 1) Raw material preparation: oats at flowering stage in Xinjin County, Chengdu in April, manually harvested and chopped to 5-8cm;

[0054] 2) Strain activation: Inoculate the Lactobacillus plantarum preserved in the ampoule into the MRS liquid medium, culture at 30°C for 12-14 hours, subculture twice to obtain the activated strain, and inoculate the activated strain into the MRS liquid medium Expand the culture, use cyclodextrin to wrap the bacteria, freeze-dry, make a solid bacteria preparation, and make the number of viable bacteria >109cfu / g;

[0055] 3) Preparation and fermentation of silage raw materials: Dilute the solid bacterial preparation with distilled water, inoculate it into the fresh grass raw material according to the inoculum amount of 100 cfu / g of viable ...

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Abstract

The invention discloses lactobacillus plantarum for high-moisture oat silage. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on May 15th, 2019,the strain is Lactobacillus plantarum, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 17740. The invention also provides application of the lactobacillus plantarum in preparation of oat silage and a preparation method of the oat silage. The strain disclosed by the invention has strong comprehensive acid production capability; rapid production oflactic acid is realized, the pH is reduced, the content of ammoniacal nitrogen is reduced, the palatability of the silage is well improved, the growth of fungi is inhibited, the aerobic stability of the silage is improved, the storage life of the silage is prolonged, the nutritional quality of the oat grass silage raw material is reserved to the maximum extent, and the prepared silage is free of any additive, good in biological safety and low in preparation cost.

Description

technical field [0001] The invention belongs to the technical field of silage, and particularly relates to a plant lactobacillus used for high-moisture oat silage, and the invention also provides an application of the lactobacillus. Background technique [0002] Silage has become an important component of ruminant diets around the world. For a long time, oats have been the main food crop and source of (silage) fodder in China's semi-arid farming and pastoral areas and high-altitude mountainous areas because of its fast growth rate, high yield, and simple and flexible cultivation methods. However, when oats are harvested, it coincides with the rainy season (April and September), coupled with imperfect harvest management measures, resulting in the oats not being able to wilt moderately under natural conditions, and having a high water content, which causes silage in the process of silage storage. The activity of acid bacteria is high, which is not conducive to the preservatio...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18C12R1/25
CPCA23K30/18C12R2001/25C12N1/205A23V2400/169
Inventor 李平张昌兵白史且陈良寅王耀华游明鸿苟民明
Owner SICHUAN ACAD OF GRASSLAND SCI
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