A strain of Lactobacillus plantarum used for high-moisture oat silage and its application

A technology of Lactobacillus plantarum and high moisture, which is applied in the field of silage, can solve the problems of high production cost and less Lactobacillus preparations, and achieve the effects of reduced content, strong acid production ability and low preparation cost

Active Publication Date: 2021-01-29
SICHUAN ACAD OF GRASSLAND SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Adding chemical additives such as formic acid, propionic acid, and potassium sorbate can improve the fermentation quality of high-moisture silage to a certain extent. However, the production cost of such additives is high, and farmers and herdsmen prefer microbial preparations. Moist oat silage with less Lactobacillus preparation

Method used

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  • A strain of Lactobacillus plantarum used for high-moisture oat silage and its application
  • A strain of Lactobacillus plantarum used for high-moisture oat silage and its application
  • A strain of Lactobacillus plantarum used for high-moisture oat silage and its application

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Embodiment 1

[0035] This embodiment is an embodiment of strain screening, determination and preservation.

[0036] A strain of Lactobacillus plantarum used for high-moisture oat silage was isolated and screened multiple times from samples such as pickles, silage, livestock manure, gastric juice, and yogurt on the Qinghai-Tibet Plateau. The Lactobacillus plantarum was preserved in China on May 15, 2019 The General Microbiology Center of the Microbial Culture Collection Management Committee, the Lactobacillus plantarum strain is Lactobacillus plantarum (L. plantarum), and the preservation number is CGMCC No.17740.

Embodiment 3

[0045] This embodiment is an application example of the plantarum lactobacillus obtained in embodiment 1 in high-moisture oat silage fermentation, and the specific method steps are as follows:

[0046] 1) Raw material preparation: Aba Hongyuan County flowering oats in September, manually harvested and chopped to 5-8cm;

[0047] 2) Strain activation: Inoculate the Lactobacillus plantarum preserved in the ampoule into the MRS liquid medium, culture at 30°C for 12-14 hours, subculture twice to obtain the activated strain, and inoculate the activated strain into the MRS liquid medium Expand the culture, use cyclodextrin to wrap the bacteria, freeze-dry, make a solid bacteria preparation, and make the number of viable bacteria >109cfu / g;

[0048] 3) Preparation and fermentation of silage raw materials: Dilute the solid bacterial preparation with distilled water, inoculate it into the fresh grass raw material according to the inoculum amount of 105 cfu / g of viable bacteria, mix it a...

Embodiment 4

[0052] This embodiment is an application example of the plantarum lactobacillus obtained in embodiment 1 in high-moisture oat silage fermentation, and the specific method steps are as follows:

[0053] 1) Raw material preparation: oats at flowering stage in Xinjin County, Chengdu in April, manually harvested and chopped to 5-8cm;

[0054] 2) Strain activation: Inoculate the Lactobacillus plantarum preserved in the ampoule into the MRS liquid medium, culture at 30°C for 12-14 hours, subculture twice to obtain the activated strain, and inoculate the activated strain into the MRS liquid medium Expand the culture, use cyclodextrin to wrap the bacteria, freeze-dry, make a solid bacteria preparation, and make the number of viable bacteria >109cfu / g;

[0055] 3) Preparation and fermentation of silage raw materials: Dilute the solid bacterial preparation with distilled water, inoculate it into the fresh grass raw material according to the inoculation amount of 100 cfu / g of viable bact...

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Abstract

The invention discloses a strain of Lactobacillus plantarum used for high-moisture oat silage, which was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 15, 2019. The strain is Lactobacillus plantarum, and the preservation number is CGMCC No.17740. The invention also provides an application of the plant lactobacillus in preparing oat silage and a preparation method of oat silage. The bacterial strain of the present invention has strong comprehensive acid production ability, can quickly generate lactic acid, lower pH, lower the content of ammoniacal nitrogen, improve the palatability of silage, inhibit the growth of fungi, help improve its aerobic stability, and prolong silage The storage period is long, the nutritional quality of oat grass silage raw materials is retained to the greatest extent, the prepared silage does not contain any additives, the biological safety is good, and the preparation cost is low.

Description

technical field [0001] The invention belongs to the technical field of silage, and particularly relates to a plant lactobacillus used for high-moisture oat silage, and the invention also provides an application of the lactobacillus. Background technique [0002] Silage has become an important component of ruminant diets around the world. For a long time, oats have been the main food crop and source of (silage) fodder in China's semi-arid farming and pastoral areas and high-altitude mountainous areas because of its fast growth rate, high yield, and simple and flexible cultivation methods. However, when oats are harvested, it coincides with the rainy season (April and September), coupled with imperfect harvest management measures, resulting in the oats not being able to wilt moderately under natural conditions, and having a high water content, which causes silage in the process of silage storage. The activity of acid bacteria is high, which is not conducive to the preservatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/25
CPCA23K30/18C12R2001/25C12N1/205A23V2400/169
Inventor 李平张昌兵白史且陈良寅王耀华游明鸿苟民明
Owner SICHUAN ACAD OF GRASSLAND SCI
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