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A kind of anti-inflammation active Phellinus polysaccharide shp and preparation method thereof

A Phellinus linteus polysaccharide and active technology, applied in the field of anti-inflammatory active Phellinus linteus polysaccharide SHP and its preparation, can solve the problems of complex polysaccharide structure, different polysaccharide activities, etc., and achieve clear structure, uniform purity, and obvious effects of experimental colitis.

Active Publication Date: 2022-04-05
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complex structure of polysaccharides, different molecular weights, different monosaccharide compositions, and different linking methods may cause different activities of polysaccharides.

Method used

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  • A kind of anti-inflammation active Phellinus polysaccharide shp and preparation method thereof
  • A kind of anti-inflammation active Phellinus polysaccharide shp and preparation method thereof
  • A kind of anti-inflammation active Phellinus polysaccharide shp and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1. Preparation of Phellinus Phellinus polysaccharide SHP with anti-inflammatory activity:

[0037] a) Raw material preparation: slice wild or artificially cultivated Phellinus chinensis fruiting bodies after harvesting, dry naturally or in an oven, and sieve after being pulverized by a pulverizer to obtain Phellinus phellifolia raw material powder. The temperature of the oven is 50°C. The sieve mesh is 40 mesh;

[0038]b) Water extraction: add Phellinus Phellinus raw material powder in step a) into water at a mass-volume ratio of 1:10-1:40, extract at 80-100°C for 4-6 hours, filter to remove the filter residue after the extraction is completed, and Collect the filtrate, then extract the filter residue again, add the filter residue to water at a mass volume ratio of 1:10 to 1:40, and extract for 2 to 3 hours at 80 to 100°C. After the extraction is completed, filter to remove the filter residue and collect the filtrate. The number of extractions is at least on...

Embodiment 2

[0047] Embodiment two, purity detection:

[0048] Such as figure 1 As shown, the high-performance liquid chromatography (Shimadzu LC-10A) equipped with BRT104-102 series gel column (8 × 300mm) (BoruiSaccharide, Biotech.Co.Ltd.) is adopted to detect the embodiment with a differential refractive index detector The purity of the prepared anti-inflammatory active Phellinus polysaccharide SHP shows a single symmetrical peak, which proves that the anti-inflammatory active Phellinus polysaccharide SHP is a homogeneous polysaccharide.

Embodiment 3

[0049] Embodiment 3, detection of monosaccharide composition:

[0050] Such as figure 2 As shown, the anti-inflammatory active Phellinus polysaccharide SHP prepared in Example 1 is detected by gas chromatography, and it can be known that the anti-inflammatory active Phellinus polysaccharide SHP is composed of arabinose, mannose, glucose and galactose in a molar ratio of 0.050:0.318: 1: A heteropolysaccharide with a composition of 0.665.

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Abstract

The invention discloses an anti-inflammatory active Phellinus polysaccharide SHP and a preparation method thereof. The anti-inflammatory active Phellinus polysaccharide SHP is composed of arabinose, mannose, glucose and galactose in a molar ratio of 0.050:0.318:1:0.665 The heteropolysaccharide of composition, molecular weight is 46kDa, the preparation method of a kind of anti-inflammatory active polysaccharide Phellinus Phellinus SHP comprises a) raw material preparation, b) water extraction, c) alcohol precipitation, d) protein removal, e) decolorization and f) purification, Phellinus crude polysaccharides were obtained from the fruiting body of Phellinus Phellinus by water extraction and alcohol precipitation, and then the protein and color of Phellinus polysaccharides were removed by Sevag method and hydrogen peroxide oxidation method, followed by ion exchange with DEAE agarose gel respectively The acidic polysaccharide component was separated by chromatography, the first elution peak was collected by dialysis membrane desalination and GPC-Autopurify automatic gel purification system purification and elution, and the finally prepared polysaccharide had uniform purity and clear structure, and was resistant to bacterial lipopolysaccharide stimulation in vitro The macrophage RAW264.7 has a significant inhibitory effect on the inflammatory response, and has a significant alleviating effect on experimental colitis in mice in vivo.

Description

【Technical field】 [0001] The invention relates to the technical field of biotechnology, in particular to the technical field of an anti-inflammatory active Phellinus polysaccharide SHP and a preparation method thereof. 【Background technique】 [0002] Sanghuang is a medicinal and edible fungus parasitic on broad-leaved trees such as mulberry and poplar. It is often used in traditional Chinese medicine to treat gynecological inflammation and intestinal diseases such as diarrhea and diarrhea. Modern medical research has proved that it has significant anti-inflammatory effects. Physiological activities such as inflammation, anti-tumor, anti-oxidation and regulation of immunity. After research, Phellinus Phellinus water extract can significantly improve chronic ulcerative colitis in mice, and the mechanism shows that it can inhibit the inflammatory factor IL by regulating the phosphorylation of IκBα in the TLR-4 / NF-κB pathway, preventing NF-κB from entering the nucleus -1β and T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P29/00
CPCC08B37/006C08B37/0003A61P29/00
Inventor 孙雨晴钟石李有贵霍进喜朱俭勋
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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