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Multifunctional trichoderma reesei strain and application thereof

A technology of Trichoderma reesei and H-LS, which is applied in the application, chemicals and enzymes for biological control, etc., to achieve the effect of inhibiting plant pathogenic bacteria, simple production process and promoting plant growth.

Active Publication Date: 2020-04-17
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the Trichoderma strains in the prior art only have one or a few functions, and there is a lack of Trichoderma strains with multiple functions in one.

Method used

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  • Multifunctional trichoderma reesei strain and application thereof
  • Multifunctional trichoderma reesei strain and application thereof
  • Multifunctional trichoderma reesei strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, the acquisition and identification of Trichoderma reesei H-LS

[0075] 1. Acquisition and naming of strain H-LS

[0076] The bacterial strain of the present invention is derived from the healthy cucumber rhizosphere soil in the plots with serious soil-borne diseases (damping-off disease) in Haidian District, Beijing. By antagonizing phytopathogenic bacteria and plant growth-promoting properties, several multifunctional bacterial strains are obtained through screening. Effect, growth rate, spore production, stress resistance, plant rhizosphere colonization ability and other characteristics, the strain with the best comprehensive effect was screened, and it was named strain H-LS.

[0077] 2. Identification of strain H-LS

[0078] 1. Morphological identification of strain H-LS

[0079] PDA solid medium: 200g potatoes (boiled and filtered to get filtrate), 20g glucose, 18g agar, 1000mL distilled water, natural pH.

[0080] See the photo of colony morphology...

Embodiment 2

[0085] Embodiment 2, the nutritional improvement ability of Trichoderma reesei H-LS

[0086] 1. Determination of phosphorus dissolving ability (that is, the ability to convert phosphorus that cannot be directly used by plants into soluble phosphorus that can be used by plants)

[0087] 1. Qualitative determination

[0088] Montina organic phosphorus solid medium: peptone 10.0g, beef extract 3.0g, NaC1 5.0g, Agar 15g, water 1000mL, pH7.0, aliquoted and sterilized, add 3mL egg yolk solution per 50mL before use (egg yolk solution is fresh Egg yolk mixed with equal volumes of 0.9% saline; egg yolk provides refractory lecithin).

[0089] Trichoderma reesei H-LS was spot-inoculated on Montina organophosphate solid medium, cultured at 28°C for 7 days, and its growth was observed.

[0090] Trichoderma reesei H-LS grows well on Montina organic medium, can produce a large number of green spores, and can form obvious phosphorus-dissolving circles, indicating that it has the ability to ...

Embodiment 3

[0118] Embodiment 3, the antagonistic ability of Trichoderma reesei H-LS to plant pathogenic fungus

[0119] 1. Determination of Chitinase Production Ability of Trichoderma reesei H-LS

[0120] Chitinase-producing medium: (NH 4 ) 2 SO 4 3g,K 2 HPO 4 1g, NaH 2 PO 4 1g, MgS0 4 ·7H 2 O 0.5g, colloidal chitin 10g, agar 18g, distilled water 1000mL, pH7.1-7.4.

[0121] Cultivate Trichoderma reesei H-LS on PDA solid medium at 28°C for 5 days, prepare bacteria cakes with a diameter of 5 mm, inoculate them in the center of the chitinase-producing medium plate, and place them upside down in the dark at 28°C for 7 days, and observe the medium There is no hydrolysis circle on it.

[0122] The results showed that Trichoderma reesei H-LS could grow on the chitinase-producing medium, produce a large number of spores, and there was a hydrolysis circle around the colony, indicating that it had the ability to produce chitinase.

[0123] Trichoderma can parasitize many other types ...

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Abstract

The invention discloses a multifunctional trichoderma reesei strain and application thereof. The multifunctional trichoderma reesei strain is trichoderma reesei H-LS, and the preservation number of the multifunctional trichoderma reesei is CGMCC No. 18108. The invention further discloses application of the trichoderma reesei H-LS in promoting plant growth and / or inhibiting phytopathogens and / or preventing and treating plant diseases caused by phytopathogen infection and / or preventing and treating nematodes and / or improving the quality of vegetables and / or forage grass and / or improving soil nutrients. The trichoderma reesei H-LS has the characteristics of being high in growth speed, large in sporulation quantity, wide in action spectrum, high in stress resistance, capable of being colonizedin quantity at the rhizosphere of a plant and the like. The invention has important value for preventing and treating related diseases caused by phytopathogens and nematodes, and has important application value for promoting the growth of vegetables and forage grass, improving the nutritional quality and improving the physical and chemical properties of soil.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a multifunctional Trichoderma reesei strain and its application. Background technique [0002] At present, the control of plant diseases and nematodes is mainly based on chemical control. Chemical pesticides not only seriously reduce the quality of vegetables and forage, pollute the environment, destroy the ecological balance, but are also unsafe for humans and animals. With people's emphasis on food safety and ecological security, biological control is one of the important strategies to realize the sustainable development of modern agriculture. In particular, using the antagonism of the beneficial microorganisms in the rhizosphere of plants that originally existed in nature to target pathogenic microorganisms and pests to control pests and diseases has a small environmental risk and is an environmentally friendly control technology. [0003] Trichoderma i...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42A01N63/38A01P21/00A01P5/00A01P3/00C05G3/60C12R1/885
CPCC12N9/2442C12Y302/01014C12N1/14C05G3/00C05B7/00C12R2001/885C12N1/145
Inventor 韩玉竹赵建军李林祥魏莹晖蔡恺张宫胤郑嘉琪
Owner SOUTHWEST UNIVERSITY
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