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A kind of in vitro fertilization fluid and preparation method thereof

A technology of receiving semen and basal fluid, which is applied in the field of assisted reproduction, can solve the problems affecting the combination probability of sperm and egg, the excessive width of chemical indicators, and the influence of egg cell survival, so as to increase the probability of sperm-egg combination, remove active oxygen, Effect of improving sperm motility and fertilization rate

Active Publication Date: 2021-11-26
佛山辅康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the nutrient composition or physical and chemical indicators of the commonly used semen are too wide, and there is no focus on the damage of active oxygen free radicals to the vitality of sperm and eggs. During the insemination process, it is easy to cause insufficient sperm capacitation, low vitality, or the survival of egg cells. Affects, thereby affecting the chances of sperm and egg combining

Method used

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  • A kind of in vitro fertilization fluid and preparation method thereof
  • A kind of in vitro fertilization fluid and preparation method thereof
  • A kind of in vitro fertilization fluid and preparation method thereof

Examples

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Embodiment 1

[0044] In this embodiment, an in vitro fertilization fluid, including base fluid, human serum albumin, coenzyme Q10 and pentoxifylline, is specifically shown in Table 1:

[0045] Table 1 In vitro semen formula of this embodiment

[0046] materials concentration NaCl 90.08mmol / L KC1 5.5mmol / L NaH 2 P0 4

0.25mmol / L MgSO 4

1mmol / L NaHCO 3

25mmol / L CaCl 2

1.8mmol / L glucose 0.5mmol / L sodium lactate 10.5mmol / L sodium pyruvate 0.32mmol / L Alanine 0.1mmol / L Aspartic acid 0.1mmol / L Asparagine 0.1mmol / L glutamic acid 0.1mmol / L Glycine 0.1mmol / L proline 0.1mmol / L serine 0.1mmol / L Alanyl-Glutamine 1mmol / L taurine 0.1mmol / L Tetracarboxylic acid ethylenediaminetetraacetic acid 0.01mmol / L HSA 10g / L Gentamicin Sulfate 50mg / L Coenzyme Q10 1mg / L Pentoxifylline 1g / L

[0047] The preparation method of above...

Embodiment 2

[0052] In this embodiment, an in vitro fertilization fluid, including base fluid, human serum albumin, coenzyme Q10 and pentoxifylline, is specifically shown in Table 2:

[0053] Table 2 In vitro semen formula of this embodiment

[0054] materials concentration NaCl 95mmol / L KC1 5.5mmol / L NaH 2 P0 4

[0055] The preparation method of the above-mentioned in vitro fertilization fluid is the same as that in Example 1.

Embodiment 3

[0057] In this embodiment, an in vitro fertilization liquid, comprising base liquid, human serum albumin, coenzyme Q10 and pentoxifylline, as shown in Table 3:

[0058] Table 3 In vitro semen formula of this embodiment

[0059] materials concentration NaCl 98mmol / L KC1 5.3mmol / L NaH 2 P0 4

[0060] The preparation method of the above-mentioned in vitro fertilization fluid is the same as that in Example 1.

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Abstract

The invention provides an in vitro fertilization liquid and a preparation method thereof, the in vitro fertilization liquid comprises basal fluid, human serum albumin, coenzyme Q10 and pentoxifylline, and the content of coenzyme Q10 in the in vitro fertilization liquid is 0.5- 2mg / L, the content of pentoxifylline in the in vitro fertilization fluid is 0.5-5g / L. The above in vitro fertilization fluid can provide a stable fertilization environment, reduce the damage of active oxygen to sperm and eggs, and can also improve sperm motility and fertilization rate.

Description

technical field [0001] The invention relates to the technical field of assisted reproduction, in particular to an in vitro fertilization fluid and a preparation method thereof. Background technique [0002] Assisted reproductive technology is the abbreviation of assisted reproductive technology (Assisted Reproductive Technology, ART), which refers to the technology of using medical assistance to make infertile couples pregnant, including artificial insemination (AI) and in vitro fertilization-embryo transfer (In Vitro Fertilization and Embryo Transfer). EmbryoTransfer, IVF-ET) and its derivative technologies. Among them, in vitro fertilization-embryo transfer (IVF-ET) refers to taking out the eggs and sperm of both patients, fertilizing them outside the body, and then transferring the fertilized eggs or embryos back to the mother's uterus to develop into fetuses. [0003] In in vitro fertilization-embryo transfer (IVF-ET), it is necessary to collect good-quality sperm and e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073C12N5/075C12N5/076
CPCC12N5/0604C12N5/0609C12N5/061C12N2500/30C12N2501/998
Inventor 曾玉洁胡威姜永存赵衡斌陈烨周星宇吴秀芝吴文林
Owner 佛山辅康生物科技有限公司
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