38 results about "Pentoxyfylline" patented technology

The invention relates to the field of analytical chemistry, in particular to a method for detecting related substances of 6-chloro-2-hexanone. The detection method adopts a gas chromatographic method for determination, and chromatographic conditions are as follows: a stationary liquid of a capillary chromatographic column is polyethylene glycol or modified polyethylene glycol; and in a heating procedure, the initial temperature is 45-55 DEG C, the temperature is increased to 220-240 DEG C at the speed of 5-15 DEG C/min, and the temperature is maintained for 5-10 min. The detection method provided by the invention is good in sensitivity, accuracy, precision, reproducibility, recovery rate and stability, and accurate and reliable in detection result, and provides a guarantee for monitoring the quality stability and clinical medication safety of pentoxifylline synthesized by taking 6-chloro-2-hexanone as a starting material.

The invention belongs to the field of medicine, in particular to a new application of human urinary kallidinogenase and a pharmaceutical composition with the human urinary kallidinogenase. According to the new application of the human urinary kallidinogenase, the human urinary kallidinogenase is used for preparing medicine for treating hypertensive nephropathy, and the test discovers that the human urinary kallidinogenase is capable of lowering the arterial pressure of a rat with hypertensive nephropathy and improving the glomerular filtration rate and renal plasma flow of the rat with hypertensive nephropathy through regulating the micro-circulation of glomerulus, and has a certain treatment effect for renal damage. The pharmaceutical composition for treating the hypertensive nephropathy comprises the human urinary kallidinogenase and betahistine hydrochloride and further comprises pentoxifylline, the pharmaceutical composition has an obvious blood pressure reducing efficiency and has an obvious treatment effect for the damaged kidney, and the pharmaceutical composition for treating the hypertensive nephropathy is definite in effect, low in side effect and better for the recovery of a patient with hypertensive nephropathy.

The invention discloses sperm diluent for donkeys. The invention discloses a donkey sperm diluent and a preparation method thereof. The composition comprises the following components: 30.4+/-0.5g of tris, 17.0+/0.2 g of citric acid; 7.5+/-0.1 g of glucose, 12.5+/-0.2 mg of taurine, 36.3+/-0.5 mg of sodium pyruvate, 1.5+/-0.1 g of fructose, 2+/-0.1 g of modified lecithin, 1.5+/-0.1 g of pentoxifylline and 25.4+/-0.3 mg of soy isoflavone which are dissolved with water for injection. By adopting the modified lecithin, the damage of irregular particles in common phospholipid to sperms is avoided,the effects of regulating osmotic pressure and protecting sperm cell membranes are achieved, and the survival rate of the sperms is increased; pentoxifylline is adopted, so that the concentration of cAMP in sperm cytoplasm is increased, cAMP dependent protease is activated, tail parts of sperms are phosphorylated, and the athletic ability of the sperms is enhanced; soybean isoflavone is adopted, excessive free oxygen of sperms in an in-vitro environment is removed, oxidative damage to the sperms is avoided, sperm motility is maintained, and the success rate of artificial insemination is increased.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a pentoxifylline injection and a preparation method thereof. The preparation method comprises the following steps of adding the osmotic pressure regulator of the prescription dosage into the water for injection which is 40-60% of the total preparation volume, stirring and dissolving, adjusting the pH value to 6.5-7.5, then adding the pentoxifylline of the prescription dosage, stirring and dissolving, supplementing the water for injection to the total preparation volume, adjusting the pH value to 6.5-7.5, filtering through a multi-stage polyether sulfone filter element of which the pore diameter is sequentially reduced, filling, and sterilizing to obtain the pentoxifylline injection. Compared with the prior art, the preparation method realizes the purpose of ensuring the product quality stability under the condition of not adding a stabilizer, the prescription is simpler, the total impurity content is less than or equal to 0.05%, the impurity content is basically not increased, the pH value is stable, the solution is always a colorless and clear solution in an accelerated six-month test process, the production cost is also reduced, the industrial production is convenient to realize, and the popularization value is relatively high.

The invention relates to the technical field of pharmaceutical analysis, and particularly discloses a method for detecting a genotoxic impurity in pentoxifylline. The detection method comprises the following steps that: a test solution and a reference solution are prepared; and the test solution and the reference solution are detected by adopting a liquid chromatography-mass spectrometry method. The chromatographic conditions of liquid chromatography are as follows: gradient elution is performed by adopting a C18 chromatographic column and taking a formic acid aqueous solution with the volume concentration of 0.1% as a mobile phase A and methanol as a mobile phase B; and the mass spectrometry adopts an ESI ion source and a positive ion detection mode. The detection method provided by the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like, can quickly and accurately detect a genotoxic impurity D in a pentoxifylline bulk drug, and accords with the guiding principle of ICH M7.

The invention discloses a prescription and a preparation method of a pH-stable pentoxifylline injection. The prescription is composed of pentoxifylline, sodium chloride, L-lysine and water for injection, and the dosage of the L-lysine is 3-7 mg/ml. After the L-lysine (pH 5.5-6.0) is added, the pH value of the pentoxifylline injection is more stable, and the L-lysine serving as a brain protective agent for systemic brain trauma, chronic tissue ischemia and ischemic diseases has a synergistic effect with the pentoxifylline, so that the effect of pentoxifylline for improving the blood flow volume of the brain and improving nutritional microcirculation of the body and oxygen supply of an ischemic area are improved. The pentoxifylline injection disclosed by the invention is simple and efficient in prescription process and good in safety.

The invention provides a nano-carrier sustained release preparation for treating cardiovascular diseases and a preparation method of the nano-carrier sustained release preparation. The nano-carrier sustained release preparation consists of freeze-dried pentoxifylline nano-carrier powder and pharmaceutic adjuvants. A weight ratio of pentoxifylline to a nano-carrier in the freeze-dried pentoxifylline nano-carrier powder is (4-5): 1; the nano-carrier is made of a composition of chitosan oligosaccharide, deoxycholic acid and albumin, wherein a weight ratio of chitosan oligosaccharide to deoxycholic acid to albumin is 1: (2-3): (3.0-3.3); a weight ratio of pentoxifylline to the pharmaceutic adjuvants is (4-7): 1; and the pharmaceutic adjuvants are composed of hydroxyethyl cellulose, aerosil and magnesium stearate, wherein a weight ratio of hydroxyethyl cellulose to aerosil to magnesium stearate is (5-6): (3-4): 1. The nano-carrier sustained release preparation for treating cardiovascular diseases provided by the invention is stable in dissolution speed, basically realizes zero-order linear dissolution, avoids burst release, greatly ensures the treatment effect of pentoxifylline, and obviously reduces adverse reactions; and after being placed for 6 months under an accelerated condition, the content of a degradation product is low, and stable quality is realized.

The invention discloses a blood vessel blocking agent bond joint fluorine-boron pyrrole derivative. The blood vessel blocking agent bond joint fluorine-boron pyrrole derivative is prepared through covalent bond joint of a near-infrared photosensitizer namely fluorine-boron pyrrole and a blood vessel blocking agent namely 2,5-pentoxifylline. Besides, the invention also discloses a nanometer diagnosis and treatment reagent obtained through self-assembling of the derivative and electron-enriched amphiphilic polymer namely methoxy-poly(ethanediol)-poly(2-diisopropylamine) methyl methacrylate, andan application of the nanometer diagnosis and treatment reagent to tumor treatment through synergism of I type photodynamic therapy and a blood vessel blocking treatment method. The blood vessel blocking agent bond joint fluorine-boron pyrrole derivative disclosed by the invention is clear in the structure of objective products, and simple in synthesis technology; the diagnosis and treatment reagent based on the objective products concurrently has I type photodynamic and pH responsiveness blood vessel blocking agent releasing properties, under passive targeting effects and fluorescence imagingmediating, the nanometer diagnosis and treatment reagent can accurately reach tumor positions, block tumor blood vessels and kill tumor cells, so that recurrence and transfer of tumors can be effectively prevented.

The invention belongs to the field of medicine synthesis, and discloses a synthesis method of pentoxifylline, which is characterized in that 4-(nitrogen methyl acyl chloride)-1-methylimidazole-5-acyl chloride and amino-5-hexanone are subjected to condensation ring closing, and the target product pentoxifylline is synthesized by only one-step reaction. The pentoxifylline synthesis method provided by the invention is simple in synthesis process, and byproducts obtained in the reaction process can be recycled to synthesize pentoxifylline again. The method is suitable for synthesizing pentoxifylline, and the synthesized pentoxifylline is used for preparing pentoxifylline for injection.

The invention discloses a preparation method of a pentoxifylline impurity. The preparation method comprises the following steps: adding theobromine into an organic solvent, stirring, and adding a certain amount of alkali; slowly dropwise adding a halogenating reagent into a reaction system for reaction under a temperature control condition; after the reaction is finished, filtering the reaction liquid, adding alkali liquor into the obtained filtrate to adjust the pH value to 10-11, adding a solvent for extraction, and separating the liquid; and washing, drying, concentrating and purifying the organic phase to obtain a target product. The method is easy to operate and can be used for quickly preparing the high-purity pentoxifylline impurity.

The invention discloses a synthesis method of pentoxifylline, which comprises the following steps: 1, synthesizing an intermediate I: adding 0.5-1.5 parts of 1, 3-bromochloropropane into 6.5-7.5 parts of absolute ethyl alcohol, 2-3 parts of anhydrous potassium carbonate and 1-2 parts of ethyl acetoacetate to carry out heating reflux reaction, and separating and purifying the reaction product to obtain the intermediate I; step 2, synthesizing an intermediate II: adding 0.5-1.5 parts of the intermediate I, 0.5-1.0 part of sodium bromide and 1-2 parts of 40-60% hydrobromic acid into the reaction container, dropwise adding concentrated sulfuric acid under a low-temperature condition until heat release is violent, carrying out heating reflux reaction, and separating and purifying a reaction product to obtain the intermediate II; and step 3, synthesizing pentoxifylline, namely dissolving 0.5-1.5 parts of theobromine in 2-3 parts of 10-30% sodium hydroxide solution, dropwise adding a mixed solution of 0.5-1.5 parts of the intermediate II and 4-5 parts of methanol, carrying out heating reflux reaction, and separating and purifying a reaction product to obtain the pentoxifylline. The synthesis process disclosed by the invention is relatively low in environmental pollution, and the synthesized pentoxifylline is relatively high in purity.

The invention discloses a preparation method of pentoxifylline powder for injection. The preparation method comprises nine steps of material preparation, concentrating, dilution, fine filtration and sterilization, preparation of penicillin bottles, preparation of rubber stoppers, crystallization, preparation of sterile powder and subpackaging. The preparation procedures are simple and easy in operations. Pentoxifylline, verapamil hydrochloride, mannitol and water for injection are sequentially concentrated and diluted in formula dosage, thereby ensuring thorough mixing of pentoxifylline, verapamil hydrochloride, mannitol and water for injection, and enhancing stability of the medicine. The invention provides the preparation method of the pentoxifylline powder for injection, and solves the technical problems of difficult preservation and easy generation of impurities at high temperature or under the light for existing pentoxifylline, thereby affecting curative effect.

The invention discloses a semen preserving fluid for improving semen preservation quality and a preparation method thereof. Comprising the following raw materials in parts by weight: 50-60 parts of glucose, 5-8 parts of glycine, 5-8 parts of citric acid, 10-15 parts of taurine, 3-5 parts of a bacteriostatic agent, 5-10 parts of modified lecithin, 7-12 parts of glycerol, 5-10 parts of pentoxifylline, 5-10 parts of soy isoflavone, 2-5 parts of self-made yolk and 15-20 parts of purified water. The method can maintain the vitality of donkey sperms for a long time, can bring more sufficient artificial insemination operation time or operability of cross-regional artificial insemination, can be widely applied to donkey sperm preservation and related embryo transplantation industries and embryo engineering, has a wide market prospect, and is worthy of popularization and application. The popularization and application of artificial insemination in a large-scale donkey farm and surrounding donkey farmers in the concentrated oestrus period of female donkeys are realized, and the utilization efficiency of the breeding male donkeys can be effectively improved.