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Freeze-dried preparation of Raman positioning nanoparticle, and preparation method and application thereof

A technology of freeze-dried preparations and nanoparticles, applied in the field of nanomaterials, to achieve the effect of simple and feasible preparation process, reduced energy consumption, and suitable for mass production

Pending Publication Date: 2020-04-24
点靓纳谱(上海)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on this, it is necessary to provide a Raman localized nanoparticle freeze-dried preparation and its preparation method and application for the problem of how to improve stability and safety.

Method used

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  • Freeze-dried preparation of Raman positioning nanoparticle, and preparation method and application thereof
  • Freeze-dried preparation of Raman positioning nanoparticle, and preparation method and application thereof
  • Freeze-dried preparation of Raman positioning nanoparticle, and preparation method and application thereof

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preparation example Construction

[0035] The preparation method of the above-mentioned Raman-localized nanoparticle freeze-dried preparation of one embodiment includes the following steps:

[0036] S10: Prepare the core-shell gold nanoparticles wrapped with the mesoporous silica layer into a suspension.

[0037] Specifically, the multi-layered core-shell gold nanoparticles wrapped with mesoporous silica layer are prepared with water for injection or physiological saline into a suspension with a concentration ranging from 0.1 nM to 3 nM, and mixed uniformly.

[0038]Among them, the preparation method of the multilayer core-shell structure gold nanoparticles wrapped with mesoporous silica layer is as follows: 1) Add 0.04 mg of gold nano-core particles into the aqueous solution of surfactant, centrifuge, and redisperse in the surfactant 2) Add 50uL 2mmol / L Raman molecular solution to gold nanocore 1, mix and shake for 30 minutes, centrifuge, and redisperse on the surface of 200uL In the aqueous solution of the a...

Embodiment 1

[0051] (1) The multilayer core-shell structure gold nanoparticles wrapped with the mesoporous silica layer obtained in the above steps are made into a 1nM suspension with water for injection, wherein the multilayer core-shell structure wrapped with the mesoporous silica layer The mass parts of gold nanoparticles are 80 parts;

[0052] (2) Add 10 parts of glycerin to the suspension obtained in step (1), and mix to obtain a mixed solution;

[0053] (3) Add 5 parts of sodium citrate to the suspension obtained in step (2), and adjust the pH value of the mixed solution to 7;

[0054] (4) Add 5 parts of sodium chloride in the mixed solution after adjusting the pH value, so that the osmotic pressure of the mixed solution is 450 / mOsnol.kg -1 ;

[0055] (5) Filter the mixed solution after adjusting the pH value and osmotic pressure with a 0.22 μm microporous membrane to obtain a sterile suspension; the pre-freezing temperature is -30°C, the primary drying temperature is -60°C, and th...

Embodiment 2

[0057] (1) The multilayer core-shell structure gold nanoparticles wrapped with the mesoporous silica layer obtained in the above steps are made into a 1nM suspension with water for injection, wherein the multilayer core-shell structure wrapped with the mesoporous silica layer The mass parts of gold nanoparticles are 100 parts;

[0058] (2) Add 10 parts of glycerin to the suspension obtained in step (1), and mix to obtain a mixed solution;

[0059] (3) Add 5 parts of sodium citrate to the suspension obtained in step (2), and adjust the pH value of the mixed solution to 7;

[0060] (4) Add 5 parts of sodium chloride in the mixed solution after adjusting the pH value, so that the osmotic pressure of the mixed solution is 450 / mOsnol.kg -1 ;

[0061] (5) Filter the mixed solution after adjusting the pH value and osmotic pressure with a 0.22 μm microporous membrane to obtain a sterile suspension; the pre-freezing temperature is -30°C, the primary drying temperature is -60°C, and t...

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Abstract

The invention relates to a freeze-dried preparation of a Raman positioning nanoparticle, and a preparation method and application thereof. The freeze-dried preparation of the Raman positioning nanoparticle comprises the following components in parts by mass: 80-100 parts of a core-shell-structured gold nanoparticle coated with a mesoporous silica layer, 0.1-20 parts of a freeze-drying protection agent, 0-10 parts of an acid-base regulator and 0-10 parts of an isoosmotic adjusting agent. According to the freeze-dried preparation of the Raman positioning nanoparticle and the preparation method and application thereof, the freeze-dried preparation is high in stability and safety and capable of being stored for a long time; and preparation method is simple and feasible in preparation process and suitable for mass production.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a Raman localized nanoparticle freeze-dried preparation and a preparation method and application thereof. Background technique [0002] Cancer is a serious threat to human health and life safety, and is one of the diseases with a high mortality rate. Surgical resection is still one of the most effective ways to treat cancer. Sentinel lymph node (Sentinel lymph node, SLN) is the first lymph node of malignant tumor lymphatic metastasis, which is of great significance to the metastasis of malignant tumor regional lymph nodes and to guide lymph node dissection. The technique of sentinel lymph node biopsy is regarded as "the most influential contribution and change in tumor surgery in the past 10 years". Sentinel lymph node biopsy can effectively avoid unnecessary large-scale lymphatic dissection while ensuring tumor safety, and reduce bleeding, nerve damage, lymphedema and ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K9/19A61K47/10A61K47/12A61K47/02A61K47/32A61K47/26A61K47/36
CPCA61K49/0013A61K49/0065A61K49/0093A61K9/19A61K47/10A61K47/12A61K47/02A61K47/32A61K47/26A61K47/36
Inventor 叶坚
Owner 点靓纳谱(上海)生物医药科技有限公司