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Preparation method of protein peptide and zinc chelate suitable for ruminants

A ruminant and chelate technology, which is applied in the field of protein peptide zinc chelate preparation, can solve the problems that are not many, and the preparation method has not been seen in research reports, and achieves the advantages of simple process flow, low cost and high production efficiency. Effect

Inactive Publication Date: 2020-04-28
陈石良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many researches and developments on protein zinc chelates in China at present, and there is no research report on the preparation method of protein zinc chelates suitable for ruminants.

Method used

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  • Preparation method of protein peptide and zinc chelate suitable for ruminants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Preparation of protein solution: Weigh 100 kg of soybean protein isolate powder (crude protein content is 92%), add 800 kg of water, stir evenly, and obtain protein solution;

[0019] (2) Enzymolysis: adjust the pH value of the protein solution to 8.0, add 1.5 kg of protease, and enzymolyze at 55°C for 8 hours, then raise the temperature of the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0020] (3) Ultrafiltration: The enzymatic hydrolysis solution is subjected to ultrafiltration through an ultrafiltration membrane device with a molecular weight cut-off of 3000Da, and the permeate is collected to obtain a protein peptide solution;

[0021] (4) Chelation reaction: Add 20 kg of zinc sulfate to the protein peptide solution, adjust the pH value of the solution to 5.5 under constant stirring, raise the temperature to 80°C, and keep it warm for 2 hours to obtain the protein peptide zinc chelation reaction solut...

Embodiment 2

[0024] (1) Preparation of protein solution: Weigh 100 kg of soybean protein isolate powder (crude protein content is 90%), add 550 kg of water, stir evenly, and obtain protein solution;

[0025] (2) Enzymolysis: adjust the pH value of the protein solution to 8.5, add 1.8 kg of protease, and enzymolyze at 52°C for 5 hours, then raise the temperature of the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0026] (3) Ultrafiltration: The enzymatic hydrolysis solution is subjected to ultrafiltration through an ultrafiltration membrane device with a molecular weight cut-off of 3000Da, and the permeate is collected to obtain a protein peptide solution;

[0027] (4) Chelation reaction: Add 25 kg of zinc sulfate to the protein peptide solution, adjust the pH value of the solution to 5.8 under constant stirring, raise the temperature to 90°C, and keep it warm for 3 hours to obtain the protein peptide zinc chelation reaction solut...

Embodiment 3

[0030] (1) Preparation of protein solution: Weigh 100 kg of soybean protein isolate powder (crude protein content is 91%), add 600 kg of water, stir evenly, and obtain protein solution;

[0031] (2) Enzymolysis: adjust the pH value of the protein solution to 8.2, add 1.62 kg of protease, and enzymolyze at 50°C for 6 hours, then raise the temperature of the enzymolysis solution to 85°C to inactivate the enzyme for 10 minutes to obtain the enzymolysis solution;

[0032] (3) Ultrafiltration: The enzymatic hydrolysis solution is subjected to ultrafiltration through an ultrafiltration membrane device with a molecular weight cut-off of 3000Da, and the permeate is collected to obtain the ultrafiltration permeate;

[0033] (4) Chelation reaction: Add 30 kg of zinc sulfate to the protein peptide solution, adjust the pH value of the solution to 6.0 under constant stirring, raise the temperature to 85°C, and keep it warm for 2.5 hours to obtain the protein peptide zinc chelation reaction ...

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Abstract

The invention relates to a preparation method of a protein peptide and zinc chelate suitable for ruminants. The preparation method mainly comprises the following steps: preparing a protein solution, carrying out enzymolysis, carrying out ultrafiltration, carrying out chelating, and carrying out spray drying, thus obtaining the protein peptide and zinc chelate. According to the method, soybean protein which is wide in source serves as a protein peptide source, inorganic zinc salt serves as a zinc source, the zinc element and the protein peptide are chelated, the technological process is simple,the cost is low, and the method is suitable for large-scale industrial production. The product prepared by the invention is high in small molecular peptide content and high in chelated zinc ion content, and is a novel high-efficiency zinc supplement agent for feed.

Description

technical field [0001] The invention relates to a preparation method of organic trace elements, in particular to a preparation method of protein peptide zinc chelate suitable for ruminants. Background technique [0002] Zinc is an essential trace element in animals. Zinc plays an extremely important role in important physiological processes such as animal growth and development, reproductive genetics, immunity, and endocrine. Zinc exists in many enzyme systems, such as carbonic anhydrase, respiratory enzyme, lactate dehydrogenate, superoxide dismutase, alkaline phosphatase, DNA and RNA polymerase, etc. Essential for the synthesis and utilization of vitamin A. Zinc has important functions such as promoting animal growth and development, increasing animal appetite, enhancing body immunity, promoting wound and wound healing, and maintaining normal male spermatogenesis. In the process of livestock and poultry production, zinc deficiency is also a relatively common nutritional ...

Claims

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Application Information

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IPC IPC(8): A23K50/10A23K20/20A23K20/147A23K10/14
CPCA23K10/14A23K20/147A23K20/30A23K50/10
Inventor 陈石良
Owner 陈石良
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