Preparation method and application of molecularly imprinted fluorescent sensor based on N-CQDs

A fluorescence sensor, molecular imprinting technology, applied in chemical instruments and methods, fluorescence/phosphorescence, nanotechnology for materials and surface science, etc. , the effect of reducing the use of expensive instruments

Inactive Publication Date: 2020-04-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many molecularly imprinted fluorescent sensors currently use some quantum dots containing toxic heavy metals as fluorescent substrates, such as the commonly used cadmium telluride quantum dots, in which the heavy metal cadmium has certain hazards.

Method used

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  • Preparation method and application of molecularly imprinted fluorescent sensor based on N-CQDs
  • Preparation method and application of molecularly imprinted fluorescent sensor based on N-CQDs
  • Preparation method and application of molecularly imprinted fluorescent sensor based on N-CQDs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of highly fluorescent nitrogen-doped carbon quantum dots

[0032] Dissolve 0.5g of citric acid and 0.5g of urea in 10mL of deionized water. After stirring until a homogeneous and transparent solution is obtained, transfer it to a stainless steel polytetrafluoroethylene-lined reaction kettle; react in an oven at 140°C for 4.0h; wait for natural cooling After reaching room temperature, the resulting dark green solution was precipitated three times with acetone and centrifuged to obtain the precipitate, which was highly fluorescent nitrogen-doped carbon quantum dots, which were recorded as N-CQDs; finally, the prepared N-CQDs were dried and ground to obtain Powder; add water and mix to obtain nitrogen-doped carbon quantum dot solution;

[0033] (2) Preparation of molecularly imprinted fluorescent sensors based on N-CQDs

[0034] Add 7.5mL cyclohexane, 1.8mL n-hexanol and 1.77mL Triton X-100 into a 25mL flask, stir magnetically at 1000rpm / min at room temper...

Embodiment 2

[0036] (1) Preparation of highly fluorescent nitrogen-doped carbon quantum dots

[0037] Dissolve 0.5g of citric acid and 0.5g of urea in 10mL of deionized water. After stirring until a homogeneous and transparent solution is obtained, transfer it to a stainless steel polytetrafluoroethylene-lined reaction kettle; react in an oven at 160°C for 4.0h; wait for natural cooling After reaching room temperature, the resulting dark green solution was precipitated three times with acetone and centrifuged to obtain the precipitate, which was highly fluorescent nitrogen-doped carbon quantum dots, which were recorded as N-CQDs; finally, the prepared N-CQDs were dried and ground to obtain Powder; add water and mix to obtain nitrogen-doped carbon quantum dot solution;

[0038] (2) Preparation of molecularly imprinted fluorescent sensors based on N-CQDs

[0039] Add 7.5mL cyclohexane, 1.8mL n-hexanol and 1.77mL Triton X-100 into a 25mL flask, stir magnetically at 800rpm / min at room temperatu...

Embodiment 3

[0041] (1) Preparation of highly fluorescent nitrogen-doped carbon quantum dots

[0042] Dissolve 0.5g of citric acid and 0.5g of urea in 10mL of deionized water. After stirring until a homogeneous and transparent solution, transfer to a stainless steel Teflon-lined reactor; react in an oven at 200°C for 4.0h. After naturally cooling to room temperature, the resulting dark green solution was precipitated with acetone three times, and centrifuged to obtain the precipitate, which was highly fluorescent nitrogen-doped carbon quantum dots, which were recorded as N-CQDs; finally, the prepared N-CQDs were dried, The powder is obtained after grinding; it is added to water and mixed to obtain a nitrogen-doped carbon quantum dot solution;

[0043] (2) Preparation of molecularly imprinted fluorescent sensors based on N-CQDs

[0044] Add 7.5mL cyclohexane, 1.8mL n-hexanol and 1.77mL Triton X-100 into a 25mL flask, stir magnetically at 600rpm / min at room temperature for 20min, then add 1...

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Abstract

The invention belongs to the technical field of preparation of novel nano materials, and relates to preparation of an N-CQDs-based molecularly imprinted fluorescent sensor and an application of the N-CQDs-based molecularly imprinted fluorescent sensor in high-selectivity detection of aspirin. The preparation method comprises the following steps: firstly, directly obtaining high-fluorescence nitrogen-doped carbon quantum dots by a one-step hydrothermal method; and secondly, preparing the N-CQDs-based molecularly imprinted fluorescent sensor by a reversed-phase microemulsion method. A simple-to-operate, green and low-cost synthesis method is established. The prepared high-fluorescence nitrogen-doped carbon quantum dots have a series of advantages of good water solubility, low toxicity, environmental friendliness, low cost, good biocompatibility and the like. Moreover, the molecularly imprinted fluorescent sensor based on the N-CQDs has good optical performance and stability, and has high-selectivity specific recognition capability on residual aspirin in biological and drug samples.

Description

technical field [0001] The invention belongs to the field of preparation of novel nanometer materials, and in particular relates to the preparation of N-CQDs molecularly imprinted fluorescent sensors and their application in highly selective detection of residual aspirin in biological and pharmaceutical samples. Background technique [0002] Aspirin (Aspirin), chemical name 2-acetoxybenzoic acid, also known as acetylsalicylic acid, belongs to non-steroidal anti-inflammatory drugs, has strong antipyretic and analgesic effects, and is widely used in the treatment of colds, colds, and headaches , neuralgia, joint pain and acute and chronic rheumatism and rheumatoid pain and other diseases. At the same time, aspirin has a highly selective and irreversible inhibitory effect on cyclooxygenase, a key enzyme that synthesizes prostaglandins on platelet membranes, and is a good antiplatelet drug. In recent years, with the wide application of aspirin in cardiovascular and cerebrovascu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/65B82Y20/00B82Y30/00
CPCG01N21/6428C09K11/65B82Y20/00B82Y30/00G01N2021/6432
Inventor 彭龙徐叶青王文娟黄婷闫永胜
Owner JIANGSU UNIV
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