Graphene nanopore sequencer and sequencing method thereof

Abstract

The invention relates to a graphene nanopore sequencer. The sequencer comprises a probe nano motion platform, an AFM probe, a nanopore chip, a hydrophobic liquid pool, and a three-dimensional nano motion platform, wherein the AFM probe is clamped on the probe nano motion platform, the hydrophobic liquid pool is fixedly arranged on the three-dimensional nano motion platform, the nanopore chip is installed in the hydrophobic liquid pool, a tip of the AFM probe is provided with a DNA tetrahedron, a solid nanopore is etched on the nanopore chip, a solution inside the hydrophobic liquid pool communicates through the nanopore, and a plurality of magnetic beads are arranged in the solution below the nanopore. The graphene nanopore sequencer can realize precise addressing of the nanopore, and controllable pore-passing of single DNA molecules, and is used for reducing a pore-passing speed of the DNA molecules and inhibiting DNA displacement thermal movement in the nanopore, so that a new methodfor realizing single base sequencing is provided.

Description

technical field [0001] The invention relates to the fields of single molecule control and molecular detection, in particular to a graphene nanopore sequencer and a sequencing method thereof. Background technique [0002] Currently, the widely accepted nanopore sequencing platforms in the market are three different types of sequencing instruments: MinION, GridION and PromethION from Oxford Nanopore Technologies (ONT). ONT sequencing is characterized by single-molecule sequencing, long sequencing read length, fast sequencing speed, real-time monitoring of sequencing data, and portable machines. Many milestone nanopore sequencing articles were published in Nature in 2018: the direct RNA sequencing article, the first nanopore human genome sequencing article, showing the most complete genome completed using a single sequencing technology to date. However, most of the above experiments are the experimental results obtained by using ONT's MinION, and ONT's instruments use biologic...

AI Technical Summary

Problems solved by technology

However, no research group has used solid-state nanopores to complete sequencing work so far

[0003] The challenges faced by solid-state nanopore sequencing mainly include: 1. Time resolution. Under the action of electric field, the speed of DNA passing through the hole is too fast to collect enough effective data points; 2. Spatial resolution. The distance between adjacent bases is only 0.34 nm, to achieve single-base resolution, the thickness of the nanopore must be less than 0.34nm, and it is necessary to ensure that only a single DNA strand passes through the nanopore to prevent other DNA strands from interfering with the collection of base data information. At the same time, it is necessary to help single DNA The chain finds the position of the nanopore within its chain length range, so that the DNA chain can pass through the nanopore smoothly and realize base signal collection

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