Bacillus altitudinis SWY137 and application thereof

A technology of bacillus highlandii and inoculum, which is applied in the field of agricultural microbiology, can solve problems such as phosphorus absorption by plants without insoluble phosphorus, and achieve the effects of improving plant disease resistance, obvious effect and increasing yield

Active Publication Date: 2020-05-05
XINJIANG GENLIDUO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports on promoting plant growth, dissolving insoluble phosphorus in soil, and promoting phosphorus uptake by plants, especially in promoting plant growth and improving crop stress resistance under high-salt conditions, and in saline-alkali land restoration.

Method used

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  • Bacillus altitudinis SWY137 and application thereof
  • Bacillus altitudinis SWY137 and application thereof
  • Bacillus altitudinis SWY137 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Isolation and identification of embodiment 1 bacterial strain SWY137

[0036] Soil was collected from the experimental demonstration base of Genliduo Biotechnology Co., Ltd. in Weixian County, Hebei Province, and 10 g of soil samples were added to a triangular flask filled with 100 ml of sterile saline. Add 1ml sample to 9ml sterile normal saline, and then dilute 10 2 , 10 3 , 10 4 Take 100 μl of the above soil suspension and spread it evenly on the plate of high-salt LB medium (yeast powder 5g / L, peptone 10g / L, NaCl 50g / L, agar 15g / L), and culture in a 28°C incubator for 48h , use a sterile toothpick to pick up a single bacterial colony, streak and purify it, and save it for later use.

[0037] Based on the physiological and biochemical characteristics of the strain SWY137 and the results of molecular biology tests, it was identified as Bacillus altitudinis. The specific identification results are as follows:

[0038] 1. Molecular biological identification

[003...

Embodiment 2

[0046] Example 2 Determination of Salt Tolerance of Bacterial Strain SWY137

[0047] First, the strains were activated on LB liquid medium, and the activated strains were inoculated into LB liquids containing 5%, 9%, 13%, 15%, and 17% NaCl (w / v) according to the inoculation amount of 1%. In the culture medium, shake culture at 28°C for 48h, and measure its OD600 value. At the same time, normal LB medium was inoculated as a control (CK). The results show that the growth of bacterial strain SWY137 is not significantly inhibited in the medium of salt concentration below 13%, and it is significantly inhibited in 15% NaCl solution ( Figure 7 ). Show that bacterial strain SWY137 can well grow in the environment of 13% salinity ( figure 1 ).

Embodiment 3

[0048] Determination of the phosphorus-dissolving ability of embodiment 3 bacterial strain SWY137

[0049] Strain SWY137 was first activated on LB medium. The activated bacterial strain is inoculated into the phosphate-dissolving medium shown in Appendix A in the general technical requirements for strain quality evaluation of NY / T 1847-2010 microbial fertilizer production (glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, calcium phytate 2g, distilled water 1000mL, pH 7.0. Solid medium: add 1.5% agar powder in proportion). Detect whether it has the function of dissolving phosphorus. After testing, the bacterial strain SWY137 has the ability to dissolve organic phosphorus (calcium phytate), and the diameter of the dissolution circle for dissolving organic phosphorus reaches 1.80cm ( figure 2 ).

[0050] After confirming that the bacterial strain SWY137 has a phosphorus-dissolving function, qu...

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Abstract

The invention discloses bacillus altitudinis SWY137 and an application thereof. The preservation number of the strain SWY137 is CCTCC NO:M 2019842, and the strain SWY137 is salt-tolerant bacillus altitudinis. The bacillus altitudinis can grow well in a 13% NaCl high-salt solution, can dissolve organic phosphorus and inorganic phosphorus which are difficult to dissolve, and can also produce variousgrowth promoting substances of cellulases, proteases, growth hormones and the like. Corn is taken as an example, the growth promoting effect of the strain SWY137 on plants is obvious, the stem heightof corn seedlings can be increased by 27.67%, the root length can be increased by 32.23%, the fresh weight of stems can be increased by 51.33%, the fresh weight of roots can be increased by 60.67%, the dry weight of the stems can be increased by 33.33%, and the dry weight of the roots can be increased by 34.88%; and besides, the effects of the strain SWY137 for promoting growth and increasing yield are not influenced by application of chemical fertilizers, and the strain SWY137 has important significance in saving phosphate fertilizer resources, enabling food grains to be subjected to continuous yield increase and protecting ecological environment of farmlands for agricultural production of China.

Description

technical field [0001] The invention relates to agricultural microbiology, in particular to a highland bacillus SWY137 and its application. Background technique [0002] Salinization is a kind of land desertification, which leads to the decline of soil fertility and difficulty in absorbing water for plant roots, which seriously affects the development of animal husbandry and agriculture. At present, more than 100 million hectares of land in the world have the problem of salinization, which causes serious losses to the world's food production every year and seriously affects the ecological balance of the region. In my country, saline-alkali soil has a wide distribution range, a large area, and many types, which has gradually become the main factor restricting the development of my country's agriculture. Studies in recent decades have shown that using biological measures to improve saline-alkali land has become one of the most concerned measures. [0003] Rhizosphere growth-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42C12N9/54C12P17/10C05F11/08C05G3/60C05G3/80C09K17/14A01N63/22A01P21/00C12R1/07C09K101/00
CPCC12N9/2437C12N9/54C12P17/10C05F11/08C09K17/16A01N63/00C09K2101/00C12R2001/07C12N1/205
Inventor 郭岩彬张云鸽王秀艳邢明振侯秀亭
Owner XINJIANG GENLIDUO BIOTECH CO LTD
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