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Growth-promoting composite endophytic bacterial agent and application thereof

A bacteriostatic agent and bacillus technology, applied in the field of microorganisms, to achieve the effects of improving photosynthetic characteristics, increasing chlorophyll content, and increasing biological enzyme activity of moso bamboo

Active Publication Date: 2020-05-12
福州睿谷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the compound endophytic agent with strong growth-promoting function for forest trees such as moso bamboo has not been reported yet.

Method used

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  • Growth-promoting composite endophytic bacterial agent and application thereof
  • Growth-promoting composite endophytic bacterial agent and application thereof
  • Growth-promoting composite endophytic bacterial agent and application thereof

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preparation example Construction

[0031] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: after activating the strain, carry out continuous two-stage seed liquid culture, insert into the fermentation medium for fermentation, and concentrate to obtain concentrated bacterial liquid; The concentrated bacterial solution of Enterobacter aerogenes CT-B09-2 and Acinetobacter calcoaceticus WYS-A01-1 was mixed with a protective agent (skimmed milk powder) and then freeze-dried to obtain the solid bacterial powder; the Bacillus amyloliquefaciens The fermentation liquid of JL-B05 and bacillus amyloliquefaciens JL-B06 is mixed with light calcium carbonate and then spray-dried to obtain the solid bacteria powder.

[0032] In the present invention, the strains are inoculated in NB medium and cultured for 15-25 hours to obtain a primary seed solution; the culture temperature is preferably 25-35°C, and the culture speed is preferably 150-350 r min -1 .

...

Embodiment 1

[0038] (1) Preparation of Enterobacter aerogenes CT-B09-2 bacterial liquid and solid bacterial powder:

[0039] Preparation of primary seed solution of Enterobacter aerogenes CT-B09-2

[0040] Activate Enterobacter aerogenes CT-B09-2 strain, pick a single colony, inoculate in 150ml NB medium (sterilize for 30min at 121°C), 28°C, 180 r min -1 Under the condition of shaking culture for 20 h, the primary seed solution was obtained.

[0041] Preparation of Secondary Seed Solution of Enterobacter aerogenes CT-B09-2

[0042] Put the first-grade seed solution into a sterile seed tank, the inoculum amount is 0.5% ~ 1.0% (V / V); the culture conditions are: manually adjust the speed and air flow of ventilation to adjust DO (DO40%), and the culture temperature is 25℃~ 35℃, the speed is 200~350r·min -1 , tank pressure 0.05MPa; cultivation time 10~15 h. OD of secondary seed solution 600 The value is between 0.6 and 0.8, at this time the strain is the strongest.

[0043] Preparation o...

Embodiment 2

[0064] The growth-promoting composite endophytic agent liquid preparation 1 prepared in Example 1 and Enterobacter aerogenes CT-B09-2, Acinetobacter calcium acetate WYS-A01-1, Bacillus amyloliquefaciens JL-B05 and Bacillus amyloliquefaciens JL - B06 single-species fermented mature bacterial liquid, respectively prepared with sterile water so that the content of viable bacteria is 1×10 8 The cfu / mL bacterial suspension was used to inoculate the moso bamboo seedlings (30mL / plant) through root irrigation, and then use the LI-6400 portable photosynthesis tester produced by the American LI-COR company to carry out the cleaning of the leaves of the moso bamboo seedlings on 15d and 30d. Photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO 2 Determination of photosynthesis indicators such as concentration.

[0065] The net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO of bamboo leaves were measured 15 d...

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Abstract

The invention provides a growth-promoting composite endophytic bacterial agent and belongs to the technical field of microorganisms. The growth-promoting composite endophytic bacterial agent comprisesenterobacter aerogenes CT-B09-2, acinetobacter calcoaceticus WYS-A01-1, bacillus amyloliquefaciens JL-B05 and bacillus amyloliquefaciens JL-B06, wherein the mass ratio of the enterobacter aerogenes CT-B09-2 to the acinetobacter calcoaceticus WYS-A01-1 to the bacillus amyloliquefaciens JL-B05 to the bacillus amyloliquefaciens JL-B06 is (3 to 5):(3 to 6):(3 to 6):(3 to 8); and the total viable bacterium concentration in the composite endophytic bacterial agent is (5 to 8)*10<8> cfu / ml or (2 to 3)*10<9> cfu / g. The growth-promoting composite endophytic bacterial agent has the advantages that thestrain has strong phosphorus-dissolving potassium-dissolving and nitrogen-fixing effects; colonization in the plant bodies can be realized; the phyllostachys edulis photosynthetic characteristics andthe biological enzyme activity are further improved; and important effects are achieved on regulating the growth and development of the phyllostachys edulis.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a growth-promoting compound endophytic agent and an application thereof. Background technique [0002] Plant endophytic bacteria refer to bacteria that live in various tissues and organs of healthy plants during a certain stage or all stages of their life history, and have established a harmonious relationship with plants. Endophytic bacteria are very rare natural biological resources because they can colonize and transmit in plants for a long time and are not easily affected by environmental conditions. They have a wide range of biological effects on plant growth and development, resistance to diseases and adverse environments. Studies have shown that plant hosts containing endophytes generally have the advantages of fast growth, resistance to adversity, and disease resistance, and have good research and development potential in agricultural production. Therefore, it is of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P21/00C12R1/01C12R1/07
CPCC12N1/20
Inventor 袁宗胜刘芳张国防黄秋良
Owner 福州睿谷生物科技有限公司
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