A method for hydrogen production by synchronous saccharification and fermentation of hau-m1 photosynthetic bacteria using alfalfa
A HAU-M1, synchronous saccharification and fermentation technology, applied in the field of biological hydrogen production by microbial fermentation, can solve the problems of reduced hydrogen production, reduced hydrogen production, and long time consumption, and achieves high-efficiency hydrogen production performance, good hydrogen production effect, well-designed effects
Active Publication Date: 2022-04-12
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology
This method has some disadvantages: 1) The inhibitory effect of hydrolyzed sugars makes it difficult to further hydrolyze cellulosic biomass; 2) It takes a long time, and the total time is equal to the hydrolysis time plus the hydrogen production time; 3) The process is complicated and easy to cause Pollution, reducing hydrogen production; 4) The process is complicated, which is not conducive to industrial production
Substrate concentration is also an important factor affecting hydrogen production by photofermentation. Low substrate concentration will limit hydrogen production because the substrate cannot provide sufficient nutrients for photosynthetic bacteria, while high substrate concentration will inhibit hydrogen production by photofermentation. decrease in hydrogen production
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[0038] A method of utilizing alfalfa to carry out HAU-M1 photosynthetic bacteria synchronous saccharification and fermentation hydrogen production method, the specific test method is as follows:
[0039] 1 Test method
[0040] (1) Pretreatment of alfalfa: dry the alfalfa, crush it to 80-100 μm, and set aside. The total solid content of the pretreated alfalfa is 97.71%, and the volatile solid content is 90.32%. The mass fraction of cellulose is 36.25%, the mass fraction of hemicellulose is 26.95%, and the mass fraction of lignin is 13.03%;
[0041] (2) Preparation of fermentation mixture: Add 100 mL of citric acid-sodium citrate buffer (pH 4.8) into a 150 mL reactor, then add alfalfa, cellulase and hydrogen-producing medium in sequence to make alfalfa The concentration of the cellulase is 10-50 g / L, so that the enzyme load of the cellulase (the enzyme load in the embodiment refers to the mass ratio of the cellulase to the alfalfa) is 0.05-0.25 g / g to obtain a fermentation mixt...
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The invention belongs to the technical field of biological hydrogen production by microbial fermentation, and in particular relates to a method for producing hydrogen by synchronous saccharification and fermentation of HAU‑M1 photosynthetic bacteria using alfalfa. In the method of the present invention, after the alfalfa is enzymatically hydrolyzed, the cellulose-degraded carbohydrates are used to provide the HAU-M1 photosynthetic bacteria group with high-efficiency hydrogen production performance. By designing different initial pH values, substrate (alfalfa) concentrations, and the mass ratio of alfalfa to cellulase, the optimal ratio was selected to obtain the optimal hydrogen production conditions. At an initial pH value of 6.90, substrate When the cellulase concentration was 31.23 g / mL and the cellulase enzyme loading was 0.13 g / g, the maximum hydrogen production was 55.81 mL / g, and the hydrogen production effect was the best. The invention is reasonable in design, clear in steps, simple and easy to implement, can realize photosynthetic flora, and fully utilize alfalfa biomass to produce hydrogen by HAU‑M1 photosynthetic flora.
Description
technical field [0001] The invention belongs to the technical field of biological hydrogen production by microbial fermentation, and in particular relates to a method for producing hydrogen by synchronous saccharification and fermentation of HAU-M1 photosynthetic bacteria using alfalfa. Background technique [0002] Alfalfa does not require additional nitrogen fertilizers from fossil fuels due to its ability to establish symbiosis with nitrogen-fixing rhizobia, which reduces dependence on non-renewable resources and reduces greenhouse gas emissions. In addition, the newly developed alfalfa varieties in recent years can grow very well in saline-alkali soil, which not only ensures a large area of crop land, but also obtains energy grass (alfalfa). Alfalfa is an important source of plant protein in animal feeding, because alfalfa is extremely rich in protein due to the high symbiotic relationship between alfalfa and nitrogen-fixing bacteria strains. Therefore, alfalfa has be...
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IPC IPC(8): C12P39/00C12P3/00C12R1/01
CPCC12P39/00C12P3/00
Inventor 路朝阳张全国胡建军张志萍王毅蒋丹萍王锴鑫郭思懿王健
Owner HENAN AGRICULTURAL UNIVERSITY