Gynostemma pentaphylla extract with lipid-lowering effect as well as preparation method and application thereof

A technology of Gynostemma pentaphyllum extract and action, applied in the field of natural product medicine, can solve problems such as no in-depth research, and achieve the effect of simple and easy preparation method

Pending Publication Date: 2020-05-15
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no high-purity gypenoside extract specially provided in the prior art, which has a good lipid-lowering effect, and there is no in-depth study on how to prepare lipid-lowering drugs from the gypenoside extract

Method used

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  • Gynostemma pentaphylla extract with lipid-lowering effect as well as preparation method and application thereof
  • Gynostemma pentaphylla extract with lipid-lowering effect as well as preparation method and application thereof
  • Gynostemma pentaphylla extract with lipid-lowering effect as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation of a Gynostemma pentaphyllum extract with lipid-lowering effect

[0057] S1: Gynostemma gynostemma 5kg, soaked in 60% ethanol at room temperature and extracted three times, once a week, filtered, concentrated under reduced pressure to obtain a density of 1.2-1.8g / cm 3 extract extract;

[0058] S2: Dissolve the Gynostemma pentaphyllum extract in water to obtain a dispersion, extract the dispersion successively with petroleum ether, ethyl acetate, and n-butanol, and extract 3 times respectively. The dispersion is mixed with petroleum ether, ethyl acetate, and , The volume ratio of n-butanol is respectively 1:1, 1:1, 1:1, after the extraction, get the n-butanol extract, decompress and reclaim the solvent to get the n-butanol part extract;

[0059] S3: Use 101-type macroporous resin to separate the extract from the n-butanol part, and use ethanol-water mixed solution for gradient elution, and the volume concentration of ethanol in the mixed solution ...

Embodiment 2

[0063] Example 2: Preparation of a Gynostemma pentaphyllum extract with lipid-lowering effect

[0064] S1: 5kg of the aerial part of Gynostemma pentaphyllum, soaked in 70% ethanol at room temperature and extracted three times, once a week, filtered, concentrated under reduced pressure to obtain a density of 1.4-1.6g / cm 3 extract extract;

[0065] S2: Dissolve the Gynostemma pentaphyllum extract in water to obtain a dispersion, extract the dispersion successively with petroleum ether, ethyl acetate, and n-butanol, and extract 3 times respectively. The dispersion is mixed with petroleum ether, ethyl acetate, and , The volume ratio of n-butanol is respectively 1:3, 1:3, 1:3, after the extraction, get the n-butanol extract, decompress and reclaim the solvent to get the n-butanol part extract;

[0066] S3: Use 101-type macroporous resin to separate the extract from the n-butanol part, and use ethanol-water mixed solution for gradient elution, and the volume concentration of ethano...

Embodiment 3

[0070] Example 3: Preparation of a Gynostemma pentaphyllum extract with lipid-lowering effect

[0071] S1: 5kg of the aerial part of Gynostemma pentaphyllum, soaked in 60% ethanol at room temperature and extracted three times, once a week, filtered, concentrated under reduced pressure to obtain a density of 1.4-1.6g / cm 3 extract extract;

[0072] S2: Dissolve the Gynostemma pentaphyllum extract in water to obtain a dispersion, extract the dispersion successively with petroleum ether, ethyl acetate, and n-butanol, and extract 3 times respectively. The dispersion is mixed with petroleum ether, ethyl acetate, and , The volume ratio of n-butanol is respectively 1:2, 1:2, 1:2, after the extraction, get the n-butanol extract, decompress and reclaim the solvent to get the n-butanol part extract;

[0073] S3: Use 101-type macroporous resin to separate the extract from the n-butanol part, and use ethanol-water mixed solution for gradient elution, and the volume concentration of ethano...

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PUM

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Abstract

The invention discloses a gynostemma pentaphylla extract and a preparation method thereof; the gynostemma pentaphylla extract contains 50-99% by mass of a saponin compound; the invention specificallydiscloses a structure of the saponin compound. The gynostemma pentaphylla extract provided by the invention has remarkable lipid-lowering activity, and the preparation method is simple and convenientand has good economic benefits and application prospects.

Description

technical field [0001] The invention belongs to the field of natural product medicines, and in particular relates to a Gynostemma pentaphyllum extract with lipid-lowering effect, a preparation method and application thereof. Background technique [0002] Gynostemma pentaphyllum (Thunb.) Makino) Cucurbitaceae, Gynostemma pentaphyllum herbaceous climbing plants. Distributed in China, India, Nepal, Laos, Vietnam, Malaysia, Indonesia (Java), New Guinea, Korea and Japan. In China, it is mainly distributed in Hunan, Hubei, Yunnan, Guangxi and other provinces, known as "Southern Ginseng". This species is used as medicine, and has pharmacological activities such as anti-tumor, regulating blood fat, lowering blood sugar, protecting the liver, anti-aging and improving immunity. [0003] With the continuous development of society and the continuous improvement of people's living standards, hyperlipidemia has become a metabolic disease with a high incidence. Studies have shown that e...

Claims

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Application Information

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IPC IPC(8): C07J17/00A61K31/704A61P3/06A23L33/105
CPCC07J17/005A23L33/105A61P3/06A23V2002/00A23V2200/3262A23V2250/21A23V2300/14
Inventor 王春华李正别松涛余河水向伟
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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