Preparation method and application of nanowire biosensor

Abstract

The invention relates to a preparation method and application of a nanowire biosensor, and belongs to the technical field of biological testing. According to the preparation method of the nanowire biosensor, the corresponding single-stranded nucleic acid probe is designed according to a target to be detected, and the target nucleic acid can be detected. Meanwhile, the continuous dynamic detectionof the target nucleic acid can be realized in situ through the nucleic acid melting and in-situ release technology of electric heating so that the concentration change condition of the target nucleicacid molecule is mastered. The nanowire biosensor prepared by the method can be used for conveniently and quickly preparing the required nanowire biosensor and is used for simply and quickly realizingunlinking and in-situ release of a biomarker and can be used for early diagnosis and prognosis of a detected person according to the concentration change of the specific biomarker aiming at the biomarker of a certain disease and can be widely applied to the fields of tumor early screening and prognosis monitoring, single cell research, embryo culture and development in assisted reproduction and the like.

Description

technical field [0001] The invention relates to a preparation method and application of a nanowire biosensor, belonging to the technical field of biological testing. Background technique [0002] The real-time detection of human biochemical indicators can provide rich health information with diagnostic value for medical clinical practice, such as multi-dimensional and multi-level information such as human metabolites, cytokines, and biomarkers. The detection of biomarkers is especially important for early-stage tumors. Screening and prognosis are of great significance. At present, biomarkers are mainly obtained by large-scale medical testing instruments or in vitro diagnostic equipment through in vitro analysis of blood, tissue fluid, urine, feces and other body fluids and excreta. It is difficult to achieve real-time detection, and there is a huge gap with the demand for continuous dynamic health information of the human body. . Therefore, it is of great significance to d...

AI Technical Summary

Problems solved by technology

The graphene FET field effect tube used in this detection method has a complex and time-consuming processing process, and there is a graphene transfer step in the processing process, which has a high failure rate, so the overall processing cost is high

Although the graphene FET field effect tube sensor has the ability to detect single nucleotide polymorphism (SNP), without other steps, it can only realize a single detection of a completely matching nucleic acid fragment, and cannot realize the detection of the same nucleic acid fragment. It is impossible to realize the detection of the dynamic change trend of the target fragment by one sensor after repeated measurements

Images