Preparation method and application of nanowire biosensor

A biosensor and nanowire technology, which is applied in the field of preparation of nanowire biosensors, can solve the problems of high failure rate, inability to realize multiple repeated measurements of the same nucleic acid fragment, and high processing cost

Active Publication Date: 2020-05-19
TSINGHUA UNIV
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Problems solved by technology

The graphene FET field effect tube used in this detection method has a complex and time-consuming processing process, and there is a graphene transfer step in the processing process, which has a high failure rate, so the overall processing cost is high
Although the graphene FET field effect tube sensor has the ability to detect single nuc

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  • Preparation method and application of nanowire biosensor
  • Preparation method and application of nanowire biosensor
  • Preparation method and application of nanowire biosensor

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Embodiment Construction

[0110] The preparation method of the nanowire biosensor proposed by the present invention, the structure of the nanowire biosensor prepared by it is as follows figure 1 with figure 2 Shown, preparation method has two kinds, wherein:

[0111] The first preparation method comprises the following steps:

[0112] (1) Preparation of silicon substrate 1:

[0113] (1-1) Rinse the silicon wafer successively with acetone and isopropanol repeatedly, and dry the surface with nitrogen;

[0114] (1-2) Put the dried silicon wafer into an oxidation furnace, grow a silicon dioxide insulating layer on the surface of the silicon wafer to obtain a silicon substrate, and the thickness of the silicon dioxide insulating layer is 100-1000 nanometers, an implementation of the present invention In the example, it is 500 nanometers;

[0115] (2) Prepare signal collection electrodes 4 and metal nanowires 6 on the silicon substrate 1 in step (1), including the following steps:

[0116] (2-1) Prepa...

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Abstract

The invention relates to a preparation method and application of a nanowire biosensor, and belongs to the technical field of biological testing. According to the preparation method of the nanowire biosensor, the corresponding single-stranded nucleic acid probe is designed according to a target to be detected, and the target nucleic acid can be detected. Meanwhile, the continuous dynamic detectionof the target nucleic acid can be realized in situ through the nucleic acid melting and in-situ release technology of electric heating so that the concentration change condition of the target nucleicacid molecule is mastered. The nanowire biosensor prepared by the method can be used for conveniently and quickly preparing the required nanowire biosensor and is used for simply and quickly realizingunlinking and in-situ release of a biomarker and can be used for early diagnosis and prognosis of a detected person according to the concentration change of the specific biomarker aiming at the biomarker of a certain disease and can be widely applied to the fields of tumor early screening and prognosis monitoring, single cell research, embryo culture and development in assisted reproduction and the like.

Description

technical field [0001] The invention relates to a preparation method and application of a nanowire biosensor, belonging to the technical field of biological testing. Background technique [0002] The real-time detection of human biochemical indicators can provide rich health information with diagnostic value for medical clinical practice, such as multi-dimensional and multi-level information such as human metabolites, cytokines, and biomarkers. The detection of biomarkers is especially important for early-stage tumors. Screening and prognosis are of great significance. At present, biomarkers are mainly obtained by large-scale medical testing instruments or in vitro diagnostic equipment through in vitro analysis of blood, tissue fluid, urine, feces and other body fluids and excreta. It is difficult to achieve real-time detection, and there is a huge gap with the demand for continuous dynamic health information of the human body. . Therefore, it is of great significance to d...

Claims

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Application Information

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IPC IPC(8): G01N27/00G01N27/02G01N27/327B82Y30/00B82Y40/00C23C14/35C23C14/16C23C14/04
CPCB82Y30/00B82Y40/00C23C14/042C23C14/16C23C14/35G01N27/00G01N27/02G01N27/3278
Inventor 王晗孙凯
Owner TSINGHUA UNIV
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