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OPR gene for regulating and controlling fatty acid of oilseed rapes, expression vector of OPR gene and application of expression vector

A technology for expressing vectors and fatty acids, which can be used in applications, genetic engineering, and plant genetic improvement. It can solve problems such as inability to synthesize, and achieve the effect of promoting upgrading and quality improvement

Active Publication Date: 2020-05-22
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it cannot be synthesized in the human body and must be ingested from the outside

Method used

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  • OPR gene for regulating and controlling fatty acid of oilseed rapes, expression vector of OPR gene and application of expression vector
  • OPR gene for regulating and controlling fatty acid of oilseed rapes, expression vector of OPR gene and application of expression vector
  • OPR gene for regulating and controlling fatty acid of oilseed rapes, expression vector of OPR gene and application of expression vector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. miRNA sequencing and screening of functional genes related to fatty acid metabolism

[0026] The near-isogenic lines of high oleic rapeseed (81.4% and 56.2% oleic acid content respectively) were provided by the National Oil Plant Improvement Center of Hunan Agricultural University. Take the seeds 20-35 days after self-pollination and mix them, place them in a sterilized mortar, and quickly grind them into fine powder. The whole operation is placed in a liquid nitrogen environment. For the remaining steps, refer to the extraction manual of the total RNA extraction kit.

[0027] There are five steps: sRNA library construction and sequencing, miRNA data analysis, differential miRNA identification and target gene prediction, qRT-PCR verification of differentially expressed miRNAs and their target genes, and expression regularity analysis of fatty acid metabolism-related differential miRNAs and their target genes .

[0028] Statistics on the length of clean reads found t...

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Abstract

The invention belongs to the genetic breeding field of crops, and particularly relates to an OPR gene for regulating and controlling fatty acid of oilseed rapes, an expression vector of the OPR gene and application of the expression vector. A base sequence of the OPR gene is represented by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4. The expression quantity of the gene is regulated and controlled by promoting different miRNA of the OPR gene, and final fatty acid components are analyzed by virtue of gas chromatography, so that a molecular mechanism and molecular biology function of theOPR gene, for regulating and controlling the components of the fatty acid, of the oilseed rapes are researched, and a potential novel gene resource is provided for cultivation of high oleic acid or other high-quality oilseed rapes.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, in particular to an OPR gene regulating rapeseed fatty acid, its expression vector and application. Background technique [0002] fatty acid( Fatty acid ) is an important substance to maintain human health. It can be divided into saturated fatty acids and unsaturated fatty acids. The composition ratio of the two has a great influence on the composition of fatty acids. Among animal and vegetable fats and oils, the content of saturated fatty acids in animal fats is high, while the content of unsaturated fatty acids in vegetable fats is high. Saturated fatty acids mainly include palmitic acid ( Palmitic acid ) and stearic acid ( Stearic acid ), excessive intake of saturated fatty acids can easily lead to increased blood cholesterol, triacylglycerol, and low-density lipoprotein cholesterol, which can cause narrowing of the arterial lumen and increase the risk of coronary heart disease...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N15/82A01H5/00A01H6/20
CPCC12N9/001C12N15/8247C12Y103/01042
Inventor 张振乾官梅常涛谭敏
Owner HUNAN AGRICULTURAL UNIV
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