Buffer solution for nucleic acid amplification colorimetric reaction and application thereof

Abstract

The invention discloses a buffer solution for nucleic acid amplification colorimetric reaction and application thereof. The buffer solution for the nucleic acid amplification colorimetric reaction comprises the following components in concentration: 0.5-10 mM of a weakly acidic substance, and 0.5-10 mM of a divalent cation salt. The buffer solution is applied to preparation of a nucleic acid amplification colorimetric reaction system and / or nucleic acid amplification fluorescence detection. The nucleic acid amplification colorimetric reaction system comprises one or more pairs of oligonucleotide primers of target nucleic acid for amplification, one or more DNA polymerases, the buffer solution for colorimetric reaction, and dNTPs. When being applied, the buffer solution can promote amplification reaction, can more efficiently realize the visual detection of nucleic acid amplification in a shorter time, and can be used for realizing fluorescence detection and colorimetric detection.

Description

technical field [0001] The invention relates to a buffer solution for nucleic acid amplification colorimetric reaction and application thereof, belonging to the field of biotechnology. Background technique [0002] Many detection methods for nucleic acid sequences and nucleic acid biological signals have been developed, including fluorescence techniques. Amplify the nucleic acid sequence in the sample by designing DNA primers, such as polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), etc. The amplification products amplified by these methods are qualitatively and quantitatively determined by fluorescent techniques such as molecular probes or embedded fluorescent molecules. However, these techniques require complex instrumentation, including optical components, an excitation source, and one or more sensors for fluorescence emission detection. Such devices are usually large, bulky and expensive. After subsequent development, lateral flow chroma...

AI Technical Summary

Problems solved by technology

However, in many current studies, the buffer used in colorimetric techniques relies on Tris buffer, and the discoloration results during actual operation are not obvious. Therefore, research on a reaction system suitable for amplification and colorimetric techniques is urgently needed.

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