Copper ion colorimetric detection test paper loaded with gold nanoparticles

A gold nanoparticle and detection test paper technology, applied in the field of analytical chemistry, can solve problems such as gastrointestinal disorders, cell damage, liver and kidney damage, etc., and achieve the effect of simple portability and rapid detection

Active Publication Date: 2020-05-22
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Copper is an essential trace element for the human body and is widely distributed in biological tissues. When the copper content in the human body is lower than the normal content, it will affect the redox reaction rate in the human body, enzyme transcription and other important metabolic processes; but when the copper content in the human body Excessive, it can promote the excessive production of reactive oxygen species in the organism, cause cell damage or induce apoptosis, cause gastrointestinal disorders in the short term, and damage the liver and kidneys in the long run

Method used

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  • Copper ion colorimetric detection test paper loaded with gold nanoparticles
  • Copper ion colorimetric detection test paper loaded with gold nanoparticles
  • Copper ion colorimetric detection test paper loaded with gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1: A copper ion colorimetric detection test paper loaded with gold nanoparticles

[0033] A test paper for colorimetric detection of copper ions loaded with gold nanoparticles, comprising the steps of:

[0034] (1) Preparation of T-shaped test paper

[0035] First use the software CorelDRAW to make graphics (such as figure 1 shown), and then use a laser engraving machine to cut Whatman No. 1 qualitative filter paper according to the pattern. Soak the cut paper pieces in ultrapure water for 12 hours, then soak them in absolute ethanol for 1 hour, remove them, and dry them in an oven at 37°C to obtain test papers for use.

[0036] (2) Preparation of gold nanoparticles

[0037] 45mL ultrapure water and 5mL HAuCl 4 (4g / L) solution was added in the Erlenmeyer flask, stirred on a magnetic stirrer until boiling, then 5mL of sodium citrate solution (38.8mM) was quickly added to the Erlenmeyer flask at one time, continued heating and stirring, and observed the soluti...

Embodiment 2

[0040] Example 2: Optimization of pretreatment of filter paper

[0041] The test paper was prepared with reference to the method of Example 1, the only difference being that ultrapure water and 1% PEG (Mw2000) solution were used to wet the test paper respectively. Specific operation: Take the washed and dried test paper, take 10 μL solution to wet the test paper, and then add 3 μL AuNPs (2.4 nmol / L) solution dropwise on the detection area of ​​the test paper, the wetting solution is ultrapure water and 1% PEG (Mw2000) solution and 1% PVP (Mw 10000) solution, and then placed at room temperature until completely dry; the washing is water washing and water and alcohol washing, wetting results see image 3 .

[0042] Depend on image 3 It can be seen that the AuNPs added dropwise are easy to aggregate on the filter paper after washing with water, and the aggregation of AuNPs can be reduced after washing with alcohol; the capillary transport of the filter paper can be reduced by ...

Embodiment 3

[0043] Embodiment 3: the selection of test strip shape

[0044] The test paper is prepared with reference to the method of Example 1, the difference is only that the shape of the test paper strip is changed in step (1). Specific operation: cut test papers of different shapes, wash with water and alcohol, and then dry them. Add 1% PVP solution dropwise to wet the test papers, add 3 μL of AuNPs (2.4 nmol / L) dropwise, and place at room temperature until dry. Add 15 μL Tris-HCl (50mmol / L, pH 7.4) buffer solution dropwise, and observe the effect of the dropping solution on the AuNPs in the detection area, see the results in Figure 4 .

[0045] according to Figure 4 It can be seen that when a square paper is used for detection, the addition of buffer can cause the AuNPs deposited in the detection area to flow to the edge, forming a "circle", and the color development results are uneven. Use a T-shaped test paper, which is divided into a detection area and a sample application are...

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Abstract

The invention discloses copper ion colorimetric detection test paper loaded with gold nanoparticles, and belongs to the technical field of analytical chemistry. The gold nanoparticles are loaded on the test paper; the test paper is T-shaped; the gold nanoparticles loaded on the test paper refer to dropwise adding an AuNPs solution into a detection area of the test paper wetted by a PVP solution. The test paper is combined with a method for colorimetrically detecting Cu < 2 + > by using gold nanoparticles, so that rapid detection of Cu < 2 + > is realized, and detection can be completed within20 minutes; the test paper for colorimetric detection of Cu < 2 + >, which is rapid, simple and high in portability, is constructed; qualitative and semi-quantitative detection of Cu < 2 + > can be realized by directly identifying color change with naked eyes, and the concentration of the lowest detection limit identified with naked eyes is 50nmol / L.

Description

technical field [0001] The invention relates to a copper ion colorimetric detection test paper loaded with gold nanoparticles, which belongs to the technical field of analytical chemistry. Background technique [0002] Copper is an essential trace element for the human body and is widely distributed in biological tissues. When the copper content in the human body is lower than the normal content, it will affect the redox reaction rate in the human body, enzyme transcription and other important metabolic processes; but when the copper content in the human body Excessive, it can promote the excessive generation of reactive oxygen species in the organism, cause cell damage or induce apoptosis, cause gastrointestinal disorders in the short term, and damage the liver and kidneys in the long run. In addition, copper is also one of the common heavy metal pollutants in the environment. Therefore, it is very important to establish an efficient, sensitive, and easy-to-operate method ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/18
CPCG01N21/78G01N33/1813G01N2021/7759
Inventor 彭池方郑蓉魏新林王正武
Owner JIANGNAN UNIV
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