Normal-temperature mechanical organ perfusate and preparation method thereof

Abstract

The invention relates to a normal-temperature mechanical organ perfusate and a preparation method thereof. The normal-temperature mechanical organ perfusate is composed of the following substances andthe balance of water based on the total volume of 1000mL and each 1g of thioredoxin peroxidase II: 4-5g of polymerized hemoglobin; 1 g of thioredoxin peroxidase II; 3000 to 5000 [mu] m of heparin sodium; 1-2 g of glucose; 0.8 to 1.5 g of sodium chloride; 0.25 to 0.3 g of cefoxitin sodium; 0.15 to 0.25 g of sodium bicarbonate; 6-10 mL of a 10% compound amino acid injection; and 0.025 to 0.03 mL of12 kinds of multivitamins for injection. In the organ perfusate, the degradation rate of the polymerized hemoglobin is remarkably reduced and the stability of the polymerized hemoglobin is remarkablyenhanced through the synergistic cooperation of the polymerized hemoglobin and thioredoxin peroxidase, so that the preservation time of an isolated organ is remarkably prolonged.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a normal-temperature mechanical organ perfusate and a preparation method thereof. Background technique [0002] Many human diseases come from the necrosis of cells, tissues, and organs, resulting in loss of function, disability, and even death. With the development of modern medicine, organ transplantation technology is becoming more and more mature. From the perspective of surgery, organ transplantation technology is perfect. , head replacement surgery is not a big problem. In fact, organ transplantation still faces various difficulties, and the preservation of isolated organs is one of them. Organ transplantation requires the transplantation of living organs. Therefore, from the time when the organ for transplantation is cut off from the donor to the time when its main blood vessels are connected to the recipient's blood vessels, it always maintains a complete anatomical structu...

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Problems solved by technology

[0012] The company previously obtained highly active oxygen-carrying polymerized hemoglobin (application number: 201910846580.9) through glutaraldehyde polymerization, and added it to the organ perfusion fluid to meet the oxygen required by the normal temperature metabolism of the organ, solving the problem of organ hypoxia. The problem of long-term storage at room temperature has improved the storage time of organs at room temperature, but sometimes it still cannot meet the actual clinical storage time needs. If the timing of donors is inconsistent, then the organ is likely to be inactivated and cannot be used for transplantation. This time limit has become a major reason for the shortage of donor organs, making it difficult for many patients to wait for transplantation.

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