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Precise pathogenic microorganism detection and killing method of intelligent bioprobe

A technology of pathogenic microorganisms and biological probes, applied in the biological field, can solve problems such as harshness, narrow acid-base range, complex programming, etc., and achieve the effect of instant detection, high sensitivity, and good universality

Pending Publication Date: 2020-06-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conditions of these synthesis methods are relatively harsh, such as the need to maintain high temperature, narrow acid-base range, complex programming, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A real-time, rapid and accurate detection method for pathogenic microorganisms based on intelligent biological probes such as in situ self-assembly programmable nucleic acid, the specific steps are as follows:

[0028] The chloroauric acid solution prepared with sterile water was used as the gold ion precursor solution in this embodiment, mixed with Escherichia coli, and then continued to add a certain concentration of DNA solution diluted with sterile water as the experimental group. Wherein the final concentration of the chloroauric acid solution is 200 μmol / L, and the final concentration of the DNA solution is 20 nmol / L. At the same time, a normal control group and a blank group were set up. Mix the above mixture thoroughly and place it on a constant temperature shaker to continue incubating for 0.5h, 1h, or 2-12h. Then take a small amount of the above-mentioned mixture and add it onto the glass slide, and seal the slide with a cover glass. Excited under a laser co...

Embodiment 2

[0031] A real-time, rapid and accurate detection method for pathogenic microorganisms based on intelligent biological probes such as in situ self-assembly programmable nucleic acid, the specific steps are as follows:

[0032] The silver nitrate solution prepared with sterile water was used as the silver ion precursor solution in this embodiment, which was mixed with Escherichia coli, and then a certain concentration of DNA solution diluted with sterile water was added as the experimental group. The final concentration of the silver nitrate solution is 200 μmol / L, and the final concentration of the DNA solution is 20 nmol / L. At the same time, a normal control group and a blank group were set up. Mix the above mixture thoroughly and place it on a constant temperature shaker to continue incubating for 0.5h, 1h, or 2-12h. Then take a small amount of the above-mentioned mixture and add it onto the glass slide, and seal the slide with a cover glass. Excited under a laser confocal ...

Embodiment 3

[0035] A real-time, rapid and accurate detection method for pathogenic microorganisms based on intelligent biological probes such as in situ self-assembly programmable nucleic acid, the specific steps are as follows:

[0036] Chlorauric acid solution and silver nitrate solution configured with sterile water are used as the gold and silver ion precursor solution of this embodiment, mixed with Escherichia coli, and then continue to add a certain concentration of DNA solution diluted with sterile water as an experiment Group. Wherein the final concentration of the chloroauric acid solution and the silver nitrate solution is 200 μmol / L, and the final concentration of the DNA solution is 20 nmol / L. At the same time, a normal control group and a blank group were set up. Mix the above mixture thoroughly and place it on a constant temperature shaker to continue incubating for 0.5h, 1h, or 2-12h. Then take a small amount of the above-mentioned mixture and add it onto the glass slide,...

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Abstract

The invention discloses a precise pathogenic microorganism detection and killing method of an intelligent bioprobe. Specially, the method comprises the following steps: sequentially adding a metallicion precursor solution of a certain concentration and a nucleic acid or target protein solution of a specific structure into a pathogenic microorganism cell suspension; and after coincubation of a certain period of time, performing in-situ self-assembly biosynthesis, so as to obtain a programmable intelligent bioprobe of related pathogenic bacteria or viruses. The probe is a fluorescent or magnetic nano probe which is subjected to in-situ biological self-assembly synthesis and has precise targeted marking and real-time rapid detection on pathogenic microorganisms, and is capable of achieving cross-scale multi-mode real-time dynamic high-sensitivity and high-specificity rapid and accurate tracking and precise killing. The method is good in biological security, and in addition, is simple, convenient and feasible and is high in sensitivity, good in specificity, rapid and visible in detection and good in universality, and solid technical support is provided for early-stage diagnosis and early-warning monitoring on related diseases caused by high pathogenic microorganisms.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a real-time, rapid and accurate detection method for pathogenic microorganisms based on intelligent biological probes such as in-situ self-assembled programmable nucleic acids. Background technique [0002] Pathogenic microorganisms have always been "invisible killers" that seriously threaten human health. Reliable, effective and rapid diagnostic methods for pathogenic microorganisms are important tools for the prevention and control of many diseases (especially epidemics caused by various coronaviruses), and are also an important means of ensuring national and international biosecurity. At present, commonly used detection techniques for pathogenic microorganisms include live cell counting, molecular biology methods, enzyme-linked immunology methods, etc. The above methods are complicated to operate, and the required immunological reagents are expensive and usually highly toxic, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12Q1/02A61L2/08C12R1/19
CPCC12Q1/10C12Q1/025A61L2/0058G01N2333/245
Inventor 王雪梅国增超曾嘉瑜姜晖
Owner SOUTHEAST UNIV