Tissue engineering bone based on multi-layer cell grid and preparation method for tissue engineering bone

Abstract

The invention discloses a tissue engineering bone based on a multi-layer cell grid and a preparation method for the tissue engineering bone. The tissue engineering bone is a mesenchymal stem cell scaffold with a three-dimensional capillary network, wherein the scaffold is a three-dimensional grid formed by three-dimensional printing of a polyion compound PIC material; and methacrylate gelatin wrapping the mesenchymal stem cells and doped with a matrigel and methacrylate gelatin wrapping vascular endothelial cells and doped with the matrigel are alternately filled among three-dimensional gridslayer by layer. According to the invention, the mesenchymal stem cells are induced to form biological bone tissues, and formation of a capillary network in the mesenchymal stem cells can be promoted at the same time, so the biological bone tissue scaffold with the three-dimensional capillary network is finally formed in vitro. The scaffold material provided by the invention has excellent toughnessand rebound resilience, and after the scaffold material is implanted into a body, the scaffold material is fixed to a bone defect in a tension state, so the defect is repaired by using an implant; the effect of distracting autogenous bone osteogenesis is achieved at the same time; and the bone defect repairing effect is improved.

Description

Technical field [0001] The invention relates to a tissue engineering bone and a preparation method thereof, in particular to a tissue engineering bone formed by a multi-layered cell grid obtained by a three-dimensional printing technology and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] Because bone tissue defects and deletions caused by congenital deformities, trauma, tumors, etc. are common clinical diseases, the functional reconstruction and repair of bone tissue defects has always been an important topic of regenerative medicine. Traditional treatment methods often use autologous or allogeneic bone grafts, but their clinical applications are often limited due to limited supply or immune rejection. Tissue engineering technology is currently the most widely used artificial bone substitute material. It is currently an effective method to prepare hydrogel scaffolds containing cells. Hydrogels are used to make cell sca...

AI Technical Summary

Problems solved by technology

Due to its good biocompatibility and other characteristics, hydrogels are used to make cell scaffolds to repair bone defects, but traditional hydrogels limit their use due to their poor mechanical properties, water absorption swelling, and easy degradation. Therefore, it is of great significance to find a new type of hydrogel material with good mechanical properties, not easy to degrade, and good biocompatibility to construct a biological cell scaffold for repairing bone defects.

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