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Preparation method for rana temporaria skin small-molecule peptide chelated iron

A technology of small molecular peptides and chelated iron, which is applied in the biological field, can solve problems affecting normal absorption, etc., and achieve the effects of improving utilization rate, high biological potency and short time consumption

Pending Publication Date: 2020-06-09
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, iron deficiency will affect the normal absorption of other trace elements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Pretreatment: wash 50 g of Rana skin, dry at 50° C. for 2.5 hours, put the Rana skin into an ultrafine pulverizer, and pass through a 200-mesh sieve to obtain 44 g of Rana skin fine powder.

[0030] (2) Decolorization: The decolorizing agent adopts hydrogen peroxide, and with the help of ultrasonic breaker, the decolorization process adopts hydrogen peroxide containing 1% H 2 o 2 0.18mol / L sodium hydroxide was used to adjust the pH, and the decolorization time was controlled at 8h.

[0031] (3) Degreasing: add 1.32 L of 20% Tween-80, defatting for 4 hours to obtain 41.8 g of Rana skin powder.

[0032] (4) Enzyme immobilization: the fixed-point immobilization of endopeptidase, serine aminopeptidase and flavor protease with the help of light radiation physical technology can improve the stability of the compound enzyme. The mass ratio of the three enzymes is 2:2:1, and the immobilization time Controlled at 3h, the immobilization temperature was controlled at 50°C. ...

Embodiment 2

[0037] (1) Pretreatment: wash 100 g of Rana skin, dry at 50° C. for 3 hours, put the Rana skin into an ultrafine pulverizer, and pass through a 200-mesh sieve to obtain 88.6 g of Rana skin fine powder.

[0038] (2) Decolorization: The decolorizing agent adopts hydrogen peroxide, and with the help of ultrasonic breaker, the decolorization process adopts hydrogen peroxide containing 1% H 2 o 2 0.18mol / L sodium hydroxide was used to adjust the pH, and the decolorization time was controlled at 9h.

[0039] (3) Degreasing: Add 886 ml of 20% Tween-80, defatting for 6 hours, to obtain 84.1 g of Rana skin powder.

[0040](4) Enzyme immobilization: the fixed-point immobilization of endopeptidase, serine aminopeptidase and flavor protease with the help of light radiation physical technology can improve the stability of the compound enzyme. The mass ratio of the three enzymes is 2:2:1, and the immobilization time Controlled at 3h, the immobilization temperature was controlled at 55°C. ...

Embodiment 3

[0045] (1) Pretreatment: wash 100 g of Rana skin, dry at 50° C. for 2.5 hours, put the Rana skin into an ultrafine pulverizer, and pass through a 200-mesh sieve to obtain 88.6 g of Rana skin powder.

[0046] (2) Decolorization: The decolorizing agent adopts hydrogen peroxide, and with the help of ultrasonic breaker, the decolorization process adopts hydrogen peroxide containing 1% H 2 o 2 0.18mol / L sodium hydroxide was used to adjust the pH, and the decolorization time was controlled at 10h.

[0047] (3) Degreasing: add 1772ml of 20% Tween-80, degrease for 5 hours to obtain 84g of Rana skin powder.

[0048] (4) Enzyme immobilization: the fixed-point immobilization of endopeptidase, serine aminopeptidase and flavor protease with the help of light radiation physical technology can improve the stability of the compound enzyme. The mass ratio of the three enzymes is 2:2:1, and the immobilization time Controlled at 4h, the immobilization temperature was controlled at 60°C.

[00...

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Abstract

The invention relates to a preparation method for rana temporaria skin small-molecule peptide chelated iron. The method uses a large amount of rana temporaria skin to conduct research on the rana temporaria skin chelated iron, not only properly handles the problem of the rana temporaria skin, but also can improve the utilization rate of the rana temporaria skin and improve economic and ecologicalbenefits. The method comprises the steps of crushing the rana temporaria skin, performing decolorization, performing degreasing, performing enzymolysis, performing ultrafiltration and performing chelation. The small-molecule peptide chelated iron is a novel generation of iron supplement products, a chitosan immobilization technology is used to stabilize enzyme activity, a double light wave-water bath composite enzyme assisted method is used to enzymatically hydrolyze the rana temporaria skin, the enzymatic hydrolysis time is short, and the efficiency is high; and the ultrafiltration method isused to prepare the small-molecule peptide solution, the small-molecule peptide solution and ferrous ions are subjected to chelation to obtain the small-molecule peptide chelated iron, the chelation can be performed without freeze-drying the peptide solution to form peptide powder, the method is simple, and the obtained small-molecule peptide chelated iron has a higher chelation rate, a higher absorption rate in human bodies, and a good iron supplementation effect; and the rana temporaria skin small-molecule peptide chelated iron prepared by the method is used in the fields of food, health care and biological pharmacy.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method of small molecular peptide chelated iron, in particular to a preparation method of small molecular peptide chelated iron of forest frog skin. Background technique [0002] Rana is a famous economic frog species in my country. Most of them live in the northeast of China, especially in Jilin Province, which has the largest number, the widest distribution and the best quality. Nowadays, wood frogs are mostly used in medicine, beauty and food industries, and have extremely high nutritional and medical value. According to reports, the annual output of Rana is very high, but most of it is used for food and the extraction of Rana oil. Due to the lack of deep processing technology for Rana products, insufficient R&D investment, and weak sustainable development capabilities, the processing and utilization of Rana extremely low. Among them, the skin of the forest f...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C12N11/10A23L33/18A23L33/165
CPCC12P21/06C12N11/10C12N9/50A23L33/18A23L33/165A23V2002/00A23V2250/1592
Inventor 卢静刘爽关爽
Owner JILIN UNIV
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