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Additive for soybean meal fermentation and application of additive in feed

An additive, soybean meal technology, applied in the application, animal feed, animal feed and other directions, can solve the problems that the compound enzyme bacteria preparation is difficult to play, the bacteria-enzyme dosage is large, and the fermentation period is long, so as to improve the digestibility of animal nutrition, The effect of short process cycle and reduction of material-to-weight ratio

Pending Publication Date: 2020-06-16
GUANGDONG VTR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is an invention that uses the mixture of bacteria and enzymes to ferment soybean meal in a solid state. The dose of bacteria and enzymes used is relatively large, which not only ignores the action conditions of the enzyme itself and the competitive inhibition relationship between microorganisms, but also makes it difficult to prepare compound enzyme bacteria. Play its due effect, and the fermentation cycle is too long, resource consumption is large

Method used

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  • Additive for soybean meal fermentation and application of additive in feed
  • Additive for soybean meal fermentation and application of additive in feed
  • Additive for soybean meal fermentation and application of additive in feed

Examples

Experimental program
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Effect test

Embodiment 1

[0029] An additive for soybean meal fermentation, including composite microorganisms and composite proteases, wherein the composite microorganisms consist of 3.0×10 9 CFU / g of Saccharomyces cerevisiae, 1.0×10 9 CFU / g of Streptococcus faecalis and 2.0×10 9 CFU / g of lactobacillus; the protease consists of 150000U / g of acid protease, 200000U / g of alkaline protease and 80000U / g of neutral protease.

[0030] A fermented soybean meal prepared by the following method:

[0031] (1) Add the compound protease and the compound microorganism into the aqueous solution, then add glucose, mix and stir until dissolved, and obtain the mixed solution of activated enzyme bacteria; the compound protease preparation, compound microorganism preparation, purified water, and glucose are in the ratio of 0.15:0.5: 250:5;

[0032] (2) Inoculate the activated enzyme-bacteria mixture into the soybean meal raw material at a ratio of 0.5:1, stir and mix evenly, and then seal and ferment at 35-37°C for 45...

Embodiment 2

[0035] An additive for soybean meal fermentation, including composite microorganisms and composite proteases, wherein the composite microorganisms consist of 3.0×10 9 CFU / g of Saccharomyces cerevisiae, 2.0×10 9 CFU / g of Streptococcus faecalis and 2.0×10 9 CFU / g of lactobacillus; the composite protease is composed of 160000U / g of acid protease, 200000U / g of alkaline protease and 120000U / g of neutral protease.

[0036] A fermented soybean meal prepared by the following method:

[0037] (1) Add the compound protease and compound microorganism into the aqueous solution, then add glucose, mix and stir until dissolved, and obtain the mixed solution of activated enzyme bacteria; compound protease, compound microorganism, purified water, and glucose are in proportion of 0.15:0.5:300: 10;

[0038] (2) Inoculate the activated enzyme-bacteria mixture into soybean meal at a ratio of 0.6:1, stir and mix evenly, and then seal and ferment at 35-37°C for 45-50 hours to obtain a fermented s...

Embodiment 3

[0041] An additive for soybean meal fermentation, including composite microorganisms and composite proteases, wherein the composite microorganisms consist of 4.0×10 9 CFU / g of Saccharomyces cerevisiae, 1.0×10 9 CFU / g of Streptococcus faecalis and 2.0×10 9 CFU / g of lactobacillus; the composite protease is composed of 150,000 U / g of acid protease, 200,000 U / g of alkaline protease and 110,000 U / g of neutral protease.

[0042] A fermented soybean meal prepared by the following method:

[0043] (1) Add the compound protease and the compound microorganism into the aqueous solution, then add glucose, mix and stir until dissolved, and obtain the mixed solution of activated enzyme bacteria; the compound enzyme preparation, the compound bacterial agent, purified water, and glucose are in proportion of 0.15:0.55: 250:5;

[0044] (2) Inoculate the activated enzyme-bacteria mixture into the soybean meal raw material at a ratio of 0.5:1, stir and mix evenly, and then seal and ferment at ...

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Abstract

The invention discloses an additive for soybean meal fermentation. The additive includes composite microorganisms and compound protease. The composite microorganisms are composed of 3.0 * 10 <9> to 4.0 * 10 <9> CFU / g of saccharomyces cerevisiae, 1.0 * 10 <9> to 2.0 * 10 <9> CFU / g of streptococcus faecalis and 1.0 * 10 <9> to 2.0 * 10 <9> CFU / g of lactobacillus; the compound protease is composed ofacid protease with a concentration of 140000 to 160000 U / g, alkaline protease with a concentration of 180000 to 220000 U / g and neutral protease with a concentration of 80000 to 120000 U / g. Accordingto the soybean meal fermentation additive provided by the invention, the contents of acid-soluble protein, crude protein, lactic acid and the like in soybean meal can be increased, anti-nutritional factors such as trypsin, beta-conglycinin and glycinin in the soybean meal are reduced, the intestinal health of animals is improved, and the healthy growth of the animals is promoted.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and more specifically relates to an additive for soybean meal fermentation and its application in feed. Background technique [0002] Soybean meal is a high-protein by-product produced by extracting soybean oil from soybeans. It is the main source of plant protein in animal diets and is widely used in livestock and poultry and aquaculture. Studies have shown that the quality of dietary protein is closely related to the healthy growth of animals. Feeding untreated soybean meal to animals often causes anti-nutritional reactions in animals (especially young animals), damages the intestinal health of animals, and reduces dietary nutrients. The digestion and absorption rate will have a negative impact on animal growth and reduce breeding benefits. [0003] Protease can decompose macromolecular proteins to generate small molecular proteins or small peptides, reduce the content of antig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K10/12A23K10/14A23K20/163A23K50/30A23K50/60
CPCA23K10/37A23K10/12A23K10/14A23K20/163A23K50/30A23K50/60A23V2400/225Y02P60/87
Inventor 彭宇崔细鹏李阳源柴毛毛周银华
Owner GUANGDONG VTR BIO TECH
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