Application of Dioscin in Controlling Pine Nematode Diseases
A technology of diosgenin and nematodes, which is applied in the application field of diosgenin in the prevention and treatment of pine nematode diseases, can solve the problems of rapid transmission, being caught off guard, and destroying the ecological environment of trees, and achieves the effect of easy degradation
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Embodiment 1
[0026] Example 1: Determination of the inhibitory activity of different diosgenins and matrine on the hatching of pine nematode eggs
[0027] Pick mature pine nematodes with consistent color and size under the dissecting microscope, disinfect them with 2% sodium hypochlorite for 3-6 minutes, rinse them with sterile water for 3 times, put them into a 20-well plate, and add 10 mature pine nematodes to each treatment , add 1ml of test diosgenin solution (I, II, III) and 1ml of test matrine solution respectively, the test solution is prepared with sterilized water, and each treatment is repeated 4 times, compared with sterile water, set 26 Cultivate at ℃, observe and record the number of hatched nematodes on 4d, 8d, and 12d respectively, and calculate the inhibition rate of hatching of nematode eggs.
[0028] Table 1: Egg hatching inhibition rate after the same concentration of dioscin and matrine are processed S: dioscin K: matrine
[0029]
[0030] The results showed that di...
Embodiment 2
[0031] Embodiment 2: Determination of the poisonous activity of diosgenin to the second instar larvae of pine nematode
[0032] Using liquid soaking method: Add 1mL of the test solution to the 20-well plate, the test solution is prepared with sterilized water, then add 30-50 nematodes for the test, place in a 26°C incubator, and wait for 1d and 2d , 3d Check and record the number of live nematodes and dead nematodes under an inverted microscope, and calculate the respective corrected mortality rates. In addition, the treated 3d were resuscitated with clear water for 2d, checked again under an inverted microscope and recorded the number of live nematodes and dead nematodes, and calculated the corrected mortality rate for 2d of resuscitation. Each treatment was repeated 4 times, and sterile water was used as control.
[0033] Table 2: Corrected mortality after the same concentration of dioscin and matrine S: dioscin K: matrine
[0034]
[0035] The results showed that the c...
Embodiment 3
[0036] Embodiment 3: forest farm carries out diosgenin control pine nematode disease test
[0037] The age of the pine tree is 20 years. The diameter at breast height is 14-20cm, and the tree height is 5-7m. The tree species are mainly black pine and masson pine.
[0038] Adopt the method of tree trunk instillation, in late March, select a drill bit with a diameter of 5mm, and use an electric drill to drill a hole at a downward angle of 45° on the trunk at a distance of 40 to 50 cm from the ground. The depth of the hole is about 5-6cm, and there is one infusion hole for each tree, and the total amount of instillation agent is 0.5ml per centimeter base diameter of the trunk. Inject 5% dioscin I, 5% dioscin II, 5% dioscin III and 5% matrine into each hole with a medical infusion set, seal the hole with mud after injection, and repeat three times for each dose.
[0039] Afterwards, the growth of the plants was regularly observed, and it was found that the pine trees treated with...
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