Saponin separation and stabilization method

A technology for stabilizing saponin, which is applied in chemical instruments and methods, organic chemistry, chemical/physical processes, etc., can solve the problems of short shelf life of saponin-related products, difficult production quality control, and high content of saponin impurities. Achieve the effects of improving the quality of saponin products, low energy consumption for solvent recovery, and short processing time

Active Publication Date: 2020-06-19
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still some problems in saponin production at present, such as: natural saponin is a mixture, and the quality of each batch of production is difficult to control; the extracted saponin has high impurity content, which affects its application in daily chemicals and high-end cosmetics; The connection bonds in some structures are unstable, easy to degrade, and the shelf life of the prepared saponin-related products is short, etc.

Method used

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  • Saponin separation and stabilization method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides a kind of saponin separation and stabilization method, such as figure 1 shown, including:

[0041] (1) Take 300g of Sapindus Sapindin crude extract, add 900g of absolute ethanol=1:3, heat to 60°C, centrifuge at 4000r / min, filter, and remove solid residue;

[0042] (2) Add 2200mL acetone to the ethanol filtrate containing saponin, let it stand for 30min, centrifuge and filter at 4000r / min for 15min, wash the precipitate with acetone, and dry to obtain saponin I;

[0043] After testing, the yield of saponin I was 9.8%, the purity was 76.5%, the surface tension was 44.3mN / m, and the critical micelle concentration was 0.3g / L;

[0044] (3) Add 2200mL ether to the filtrate obtained in step (2), let stand for 60min, centrifuge and separate at 4000r / min for 15min, wash the precipitate with acetone and ether successively, and dry to obtain saponin II;

[0045] After testing, the yield of saponin II was 17.6%, the purity was 75.8%, the surface tension w...

Embodiment 2

[0049] The present embodiment provides a saponin separation and stabilization method, comprising:

[0050] (1) Take 300g of Sapindus Sapindin crude extract, add 900g of absolute ethanol=1:3, heat to 60°C, and centrifuge at 4000r / min to remove solid residue;

[0051] (2) Add 2200mL acetone to the saponin ethanol solution, let it stand for 30min, centrifuge and filter at 4000r / min for 15min, wash the precipitate with acetone, and dry to obtain saponin I;

[0052] After testing, the yield of saponin I was 9.8%, the purity was 76.5%, the surface tension was 44.3mN / m, and the critical micelle concentration was 0.3g / L;

[0053] (3) Add 2200 mL of absolute ethanol solution to the above-mentioned filtrate, let it stand for 60 minutes, centrifuge at 4000 r / min and separate for 15 minutes, wash the precipitate with acetone and ether in sequence, and dry to obtain saponin II;

[0054] After testing, the yield of saponin II was 17.6%, the purity was 75.8%, the surface tension was 49.2mN / ...

Embodiment 3

[0058] The present embodiment provides a saponin separation and stabilization method, comprising:

[0059] (1) Take 300g of Sapindus Sapindin crude extract, add 900g of absolute ethanol=1:3, heat to 60°C, and centrifuge at 4000r / min to remove solid residue;

[0060] (2) Add 2200mL acetone to the saponin ethanol solution, let it stand for 30min, centrifuge and filter at 4000r / min for 15min, wash the precipitate with acetone, and dry to obtain saponin I;

[0061] After testing, the yield of saponin I was 9.8%, the purity was 76.5%, the surface tension was 44.3mN / m, and the critical micelle concentration was 0.3g / L;

[0062] (3) Add 2200 mL of absolute ethanol solution to the above-mentioned filtrate, let it stand for 60 minutes, centrifuge at 4000 r / min and separate for 15 minutes, wash the precipitate with acetone and ether in sequence, and dry to obtain saponin II;

[0063] After testing, the yield of saponin II was 17.6%, the purity was 75.8%, the surface tension was 49.2mN / ...

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Abstract

The invention belongs to the technical field of high-quality utilization of plant extracts, and particularly relates to a saponin separation and stabilization method. The saponin separation and stabilization method comprises the following steps: crude saponin is used as a raw material and filtering by absolute ethyl alcohol, obtained filtrate I is subjected to acetone precipitation separation to obtain saponin I, obtained filtrate II is subjected to acetone / diethyl ether precipitation separation to obtain saponin II, obtained filtrate III is further subjected to diethyl ether precipitation separation, obtained precipitation filter residues are dissolved in water and then subjected to heat treatment and acid stabilization treatment, and thus saponin III is obtained. According to the method,after the crude saponin is filtered by absolute ethyl alcohol, acetone, acetone / diethyl ether and diethyl ether are sequentially used for fractional purification so that three saponin products with different properties can be obtained at the same time, and a basis is provided for refined and high-quality utilization of saponin; in addition, saponin obtained through diethyl ether precipitation issubjected to stabilizing treatment and the stability is remarkably improved, the critical micelle concentration and surface tension of saponin are remarkably reduced, and the quality of saponin products is improved.

Description

technical field [0001] The invention belongs to the technical field of high-quality utilization of plant extracts, and in particular relates to a saponin separation and stabilization method. Background technique [0002] Natural saponin is a general term for plant glycosides that can form aqueous or colloidal solutions and form soapy foam. It is composed of saponin and sugar, uronic acid or other organic acids, and can effectively reduce the surface of water. Tension is a natural, non-toxic, non-polluting surfactant. The main source plants of saponin include Sapindus chinensis, camellia oleifera and acacia, etc. [0003] Sapindia chinensis pericarp contains 20-25% saponin, acacia pericarp contains 36-33% saponin, and camellia oleifera cake contains about 10% saponin. These are ideal raw materials for extracting natural saponin. Some enterprises have established saponin extraction production lines, and some products have also been promoted and applied. [0004] However, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/00B01F17/56C07G3/00C09K23/00C09K23/56
CPCC07G3/00C09K23/018
Inventor 蒋建新张威伟刘传杰朱莉伟
Owner BEIJING FORESTRY UNIVERSITY
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