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Method for detecting liquid sample based on terahertz technology

A liquid sample, technical detection technology, applied in the field of biomedicine, can solve the problems of uneven samples, inaccurate measurement results, small measurement area, etc., and achieve the effect of improving sensitivity and applicability

Pending Publication Date: 2020-06-19
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for detecting liquid samples based on terahertz technology, so as to solve the problem of inaccurate measurement results caused by the small measurement area and uneven samples of terahertz spectral biological detection technology in the prior art

Method used

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  • Method for detecting liquid sample based on terahertz technology
  • Method for detecting liquid sample based on terahertz technology
  • Method for detecting liquid sample based on terahertz technology

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1 Combining the I-shaped metamaterial sensor and reverse micelles to detect the conformation of coagulation factor eight protein

[0044] A preferred embodiment of the method according to the present invention for detecting the properties of biomolecules is to detect the overall conformational change of coagulation factor VIII protein. Wherein, the present embodiment test sample such as image 3 , the dispersed phase is composed of blood coagulation factor 4 dissolved in phosphate buffered saline solution 5 at pH = 7.3, and the surfactant is 1,2-dioleoyl-sn-glycerol-3-phosphatidylcholine 15, which is used to test calcium ion coagulation The influence of the overall conformation of the eight-factor protein, the control group also contains 2mM CaCl 2 . Specifically, the preparation method of the test sample is as follows: 1,2-dioleoyl-sn-glycerol-3-phosphatidylcholine 15 dissolved in chloroform is blown dry with nitrogen, and the solvent is removed by vacuum to ...

Embodiment 2

[0050] Example 2 Combining double-strip metamaterial sensors and reverse micelles to detect the conformation of lectin protein

[0051] A preferred embodiment of the method according to the invention for detecting properties of biomolecules is the detection of global conformational changes of the lactadherin protein. Wherein, the present embodiment test sample such as Figure 7 , the dispersed phase is composed of lectin 19 dissolved in phosphate buffered saline solution 5 with pH=7.3, and the surfactant is 1,2-dioleoyl-sn-glycerol-3-phosphatidylcholine 15, which is used to test calcium ion coagulation The influence of the overall conformation of the eight-factor protein, the control group also contains 2mM CaCl 2. The preparation method of the test sample is as follows: 1,2-dioleoyl-sn-glycerol-3-phosphatidylcholine 15 dissolved in chloroform is blown dry with nitrogen, and vacuumized to remove the solvent to form a film; Phospholipids were dissolved in hexadecane; lactagg...

Embodiment 3

[0053] Example 3 Based on the I-shaped metamaterial sensor and reverse micelles to detect the binding effect of blood coagulation factor eight protein and phospholipid membrane protein

[0054] A preferred embodiment of the method for detecting biomolecular interactions according to the present invention is to detect coagulation factor VIII protein 4 and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine loaded with lipid-anchored membrane protein 20 15 Binding role of phospholipid membranes. The prepared reverse micelles samples were as Figure 9 . Among them, the dispersed phase is composed of blood coagulation factor eight protein 4 dissolved in phosphate buffered saline solution 5 at pH = 7.3; 20 is composed of a mass ratio of 20:1, which is used to detect the binding status of lipid-anchored membrane protein 20 with different contents. The sample preparation method is as follows: 1,2-dioleoyl-sn-glycerol-3-phosphatidylcholine 15 dissolved in chloroform was blown dry with ni...

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Abstract

The invention provides a method for detecting a liquid sample based on a terahertz technology, and the method comprises the steps: S1, providing a terahertz spectrum device, a container capable of accommodating the liquid sample, and a metamaterial sensor made of a terahertz metamaterial, wherein the metamaterial sensor is clung to the inner wall of one side of the container; S2, preparing to-be-detected biomolecules into a reverse micelle emulsion and adding the biomolecules into the container, wherein the reverse micelle emulsion is an emulsion with a continuous phase as an oil phase, a dispersion phase as a water phase and phospholipid molecules as a surfactant, the phospholipid molecules exist in a reverse micelle form, and the to-be-detected biomolecules are wrapped in the dispersionphase; and S3, acquiring spectral information of the to-be-detected body in a terahertz waveband by using a terahertz spectrum device so as to realize detection of the liquid sample. According to theinvention, a terahertz spectrum device is utilized, and based on the combination of a terahertz metamaterial and reverse micelles, the properties and interaction of the biomolecules are detected and quantitative detection is carried out in a microenvironment close to physiological conditions and in a phospholipid self-assembly form.

Description

technical field [0001] The invention relates to the field of biomedical technology, and more particularly to a method for detecting liquid samples based on terahertz technology. Background technique [0002] Terahertz generally refers to electromagnetic waves with a frequency of 0.3 to 10 THz and a wavelength of 30 μm to 1 mm. It lies between the microwave and infrared bands on the electromagnetic spectrum. Terahertz time-domain spectroscopy (THz-TDS) technique is a powerful tool to study the dielectric relaxation of water molecules at biological interfaces, due to its temporal measurement scale in the range of 10 -9 to 10 -11 S, capable of characterizing direct information on picosecond hydration dynamics, is an ideal tool for studying bound water. This means that the application of the THz band to the environment full of interfacial water and organic biomolecules (such as DNA, protein, etc.) has unique advantages. At present, the application of terahertz technology to ...

Claims

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Application Information

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IPC IPC(8): G01N21/3586G01N21/3577
CPCG01N21/3586G01N21/3577
Inventor 吕军鸿唐朝胡钧
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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