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A method for rapid determination of squalene

A technology for rapid determination and squalene, applied in the field of detection of chemical components in plants, can solve problems such as no relevant literature reports, and achieve the effects of shortening the saponification reaction time, reducing the analysis cost, and improving the detection speed.

Active Publication Date: 2022-05-10
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no relevant literature report on the analysis of squalene in passion fruit seed oil

Method used

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  • A method for rapid determination of squalene
  • A method for rapid determination of squalene
  • A method for rapid determination of squalene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. The preparation of the test solution comprises the following steps:

[0036] A. Take 3g of passion fruit seed powder and place it in an ultrasonic-microwave combined extractor, add 40g of petroleum ether, and then extract under ultrasonic-microwave cooperation. The extraction conditions are ultrasonic power 200W, microwave power 150W, and temperature 35°C , the extraction time is 3 minutes, then the extract is cooled to room temperature, the residue is repeatedly extracted with 40g of petroleum ether after filtration, the extract is combined, and the solvent is evaporated to recover the oil sample;

[0037] B. Take an oil sample and a potassium hydroxide solution with a concentration of 1.0mol / L and place it in an ultrasonic-microwave combined extraction apparatus. The weight ratio of the oil sample to the potassium hydroxide solution is 1:400, and carry out saponification reaction under the cooperation of ultrasonic-microwave For 5 minutes, the saponification reacti...

Embodiment 2

[0050] 1. The preparation of the test solution comprises the following steps:

[0051]A. Take 8g of passion fruit seed powder and place it in an ultrasonic-microwave combined extractor, add 80g of petroleum ether, and then extract under ultrasonic-microwave cooperation. The extraction conditions are ultrasonic power 300W, microwave power 250W, and temperature 45°C , the extraction time was 10 minutes, then the extract was cooled to room temperature, the residue was repeatedly extracted with 80 g of petroleum ether after filtration, the extract was combined, and the solvent was evaporated and recovered to obtain an oil sample;

[0052] B. Take an oil sample and a potassium hydroxide solution with a concentration of 1.0mol / L and place it in an ultrasonic-microwave combined extraction apparatus. The weight ratio of the oil sample to the potassium hydroxide solution is 5:400, and carry out saponification reaction under the cooperation of ultrasonic-microwave For 30 minutes, the sa...

Embodiment 3

[0065] 1. The preparation of the test solution comprises the following steps:

[0066] A. Take 5g of passion fruit seed powder and place it in an ultrasonic-microwave combined extractor, add 65g of petroleum ether, and then extract under ultrasonic-microwave cooperation. The extraction conditions are ultrasonic power 250W, microwave power 200W, and temperature 40°C , the extraction time was 5 minutes, then the extract was cooled to room temperature, the residue was repeatedly extracted with 70 g of petroleum ether after filtration, the extract was combined, and the solvent was evaporated and recovered to obtain an oil sample;

[0067] B. Take an oil sample and a potassium hydroxide solution with a concentration of 1.0mol / L and place it in an ultrasonic-microwave combined extraction apparatus. The weight ratio of the oil sample to the potassium hydroxide solution is 3:400, and carry out saponification reaction under the cooperation of ultrasonic-microwave For 10 minutes, the sa...

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Abstract

The invention discloses a method for rapidly determining squalene. The method for rapidly determining squalene is determined by high-performance liquid chromatography based on microwave-ultrasonic collaborative and efficient extraction of passion fruit seed oil. The chromatographic conditions are: Chromatographic column: octadecyl bonded silica gel column; mobile phase: methanol-water volume percentage 96-100:1-3; detector: diode array detector detection wavelength 190-200 nm; flow rate 0.6-1.0 ml / min ; Detection time: 3.5‑6 minutes. The purpose of the present invention is to create a method for rapid determination of squalene, and measure squalene in passion fruit seed oil, in order to provide a theory for the nutritional evaluation of passion fruit seed oil and the research and development of passion fruit seed oil products in accordance with.

Description

technical field [0001] The invention relates to the field of a method for detecting chemical components in plants, in particular to a method for rapidly determining squalene. Background technique [0002] Squalene, also known as squalene, chemically named 2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, is composed of six Triterpenoids formed by linking isoprene head to tail have various biological activities such as oxygen carrying, anti-radiation, anti-tumor, and regulation of lipid metabolism. Because squalene has a variety of important biologically active functions, squalene is widely used in the development of pharmaceutical products, health food and cosmetics. Passion fruit is an economic fruit that grows in tropical and subtropical regions. Passion fruit seed oil is a kind of high-quality vegetable oil, which is rich in unsaturated fatty acids, among which the content of linoleic acid and oleic acid is more than 85%, and it also contains flavonoids, β-ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N2030/047G01N2030/062G01N2030/067
Inventor 刘纯友黄承都许金蓉
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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