Method for extracting tryptophan from fermentation broth

A fermentation broth, tryptophan technology, applied in the direction of organic chemistry, can solve the problems of increasing the burden of subsequent processes, increasing the volume of materials, increasing production costs, etc., to reduce the consumption of water resources and heat energy, improve product yield, save Effects of water and heat

Active Publication Date: 2020-07-03
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the content of tryptophan in the fermentation broth, a large amount of water needs to be added to dissolve the crystals, which will increase the water consumption, increase the vol

Method used

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  • Method for extracting tryptophan from fermentation broth
  • Method for extracting tryptophan from fermentation broth
  • Method for extracting tryptophan from fermentation broth

Examples

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Example Embodiment

[0041] Example 1: A method for extracting tryptophan from fermentation broth, this example does not add a crystallization aid, the specific steps are:

[0042] After the fermentation is over, immediately take 30L of fermentation broth, the content of L-tryptophan in the fermentation broth is 51.6g / L, control the stirring speed to 100 rpm, adjust the pH value of the fermentation broth for isoelectric crystallization, specifically: Sulfuric acid is added to the fermentation broth to adjust the pH of the fermentation broth to 5.90±0.02 at a rate of 0.1 per 5 minutes. After the pH is reached, the pH is maintained for 15 minutes. The isoelectric crystallization temperature is 40℃; after the isoelectric crystallization, the temperature drops and the temperature gradient is lowered. In order to reduce the temperature by 3°C per hour, the temperature is lowered to 10°C and maintained for 30 minutes. Use an automatic slag filter to filter and separate the crystals. Add 6L of ethanol with ...

Example Embodiment

[0049] Embodiment 2: A method for extracting tryptophan from fermentation broth. In this embodiment, 100 ppm of crystallization aid is added. The specific steps are:

[0050] After the fermentation is over, immediately take 30L of fermentation broth, the content of L-tryptophan in the fermentation broth is 50.4g / L, control the stirring speed to 100 rpm, add 100ppm fatty acid glycerides, adjust the pH value of the fermentation broth for isoelectric Crystallization is specifically: adding sulfuric acid to the fermentation broth to adjust the pH of the fermentation broth to 5.90±0.02 at a rate of 0.1 per 5 minutes. After the pH is reached, it is maintained for 15 minutes, and the isoelectric crystallization temperature is 40°C; after isoelectric crystallization Begin cooling crystallization, the cooling gradient is 3℃ per hour, and the temperature is reduced to 10℃ and maintained for 30 minutes. Use an automatic slag filter to filter and separate the crystals. Add 6L of ethanol with...

Example Embodiment

[0057] Embodiment 3: A method for extracting tryptophan from fermentation broth. In this embodiment, 250 ppm of crystallization aid is added, and the specific steps are:

[0058] After the fermentation is over, immediately take 30L of fermentation broth, the content of L-tryptophan in the fermentation broth is 52.6g / L, control the stirring speed to 200 rpm, add 250ppm fatty acid glycerides, adjust the pH value of the fermentation broth for isoelectric Crystallization is specifically: adding sulfuric acid to the fermentation broth to adjust the pH of the fermentation broth to 5.90±0.02 at a rate of 0.1 per 5 minutes. After the pH is reached, it is maintained for 15 minutes, and the isoelectric crystallization temperature is 38°C; after isoelectric crystallization Begin cooling and crystallization, and the cooling gradient is 3°C per hour until the temperature is 15°C and maintained for 30 minutes. Use an automatic slag filter to filter and separate the crystals, add 6L of ethanol ...

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Abstract

The invention discloses a method for extracting tryptophan from fermentation broth. The method comprises the following steps: (1) primary crystallization of fermentation broth; (2) separation to obtain primary crystals; (3) secondary crystallization of fermentation broth; and (4) obtaining a tryptophan finished product. According to the method, primary crystals are separated from tryptophan fermentation broth, then thalli and proteins in the fermentation broth are removed, the obtained clear liquid is subjected to evaporation and concentration after being discolored, then crystallization and separation are performed to obtain secondary crystals; an ion exchange procedure is completely omitted, pollutant emission is little, and the method is environmentally friendly. No organic membrane process is adopted, equipment investment is low, and the cost is low. Under the condition that the cost is reduced and the consumption of water resources and heat energy is reduced, the purity and yieldof the product are greatly improved compared with those of an existing process; and the process is suitable for large-scale industrial production.

Description

Technical field: [0001] The invention relates to a method for producing tryptophan, in particular to a method for extracting tryptophan from fermentation broth. Background technique: [0002] As an essential amino acid for human and animal life activities, tryptophan is widely used in food, feed, medicine, agriculture and forestry industries. Adding tryptophan to food can promote protein absorption by the body. As a feed additive, adding tryptophan to low-protein diets is very effective in increasing the weight gain of livestock and poultry and improving feed efficiency. Tryptophan is the fourth largest feed additive after lysine, threonine, and methionine. Amino acids. The metabolite 5-hydroxytryptophan of tryptophan has anti-high density, weaning and other stress effects in animals, promotes the production of R-globulin, and enhances the body's disease resistance. In addition, tryptophan also has an impact on the behavior of livestock and poultry, which can reduce aggressive...

Claims

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Application Information

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IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 吴涛王海雷常利斌龚华李岩赵津津刘月明
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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