Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of Bacillus licheniformis and its application

A technology of Bacillus licheniformis and strains, applied in application, bacteria, microorganisms and other directions, can solve problems such as affecting application, poor water solubility or stability, and low efficacy, achieve good economic and social benefits, and improve disease resistance. Ability, health-enhancing effect

Active Publication Date: 2021-01-15
LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of traditional Chinese medicine, the content of active ingredients is low, the water solubility or stability of some active ingredients of traditional Chinese medicine is not good, and the toxicity and side effects are too strong, resulting in low drug efficacy, which seriously affects their application and cannot achieve better results. therapeutic or health effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Bacillus licheniformis and its application
  • A strain of Bacillus licheniformis and its application
  • A strain of Bacillus licheniformis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and identification of Bacillus licheniformis LZZT-002

[0021] The soil samples of Astragalus root were collected, and the microbial strains were isolated and purified by the dilution plate method. Using culture characteristics and morphological observation to pick out multiple microbial strains, and identify the strains by Gram staining method and 16s rRNA gene rapid amplification and sequencing method. The 16s rRNA gene was identified using the strain DNA as the template, and its 16S rDNA sequence was amplified by PCR. The primers were (27f): 5'-AGAGTTTGATCCTGGCTCAG-3', SEQ ID NO. 1; and (1492r): 5'-GGTTACCTTGTTACGACTT-3', SEQ ID NO. 2. The PCR reaction program was: pre-denaturation at 95°C for 6 minutes, denaturation at 95°C for 30 seconds, annealing at 53°C for 30 seconds, extension at 72°C for 70 seconds, 30 cycles. The amplified product was sequenced after its purity was checked by electrophoresis. The obtained 16S rRNA gene sequences were co...

Embodiment 2

[0022] Example 2 Detection of xylanase activity of strain LZZT-002

[0023] After the LZZT-002 strain was fermented for two days in liquid medium (tryptone 5g, oat xylan 5g, glucose 5g, sodium chloride 5g, distilled water 1000ml, pH7.2), the cells were collected by centrifugation and placed in PBS buffer , use an ultrasonic disruptor at a power of 600W to disrupt the cells, and then centrifuge to obtain the supernatant, that is, to obtain the crude enzyme solution.

[0024] The detection of xylanase activity refers to the national standard (GB / T 23874-2009).

[0025] Standard curve preparation: draw 4ml of 0.1M acetic acid-sodium acetate buffer (pH5.5), add 5.0ml of DNS reagent, heat in a boiling water bath for 5 minutes, cool to room temperature, add water to make up to 25ml, and prepare a standard blank. Xylan standards were prepared at 0.1 mg / ml, 0.2 mg / ml, 0.3 mg / ml, 0.4 mg / ml, 0.5 mg / ml, 0.6 mg / ml and 0.7 mg / ml. Take 2ml of each standard solution, add 2ml of 0.1M acetic...

Embodiment 3

[0029] Example 3 Detection of cellulase activity of strain LZZT-002

[0030] Strain LZZT002 was seeded on sodium carboxymethylcellulose medium and cultured for 48 hours. Stained with 0.2% Congo red for 30 minutes, then washed with distilled water to remove the dye, then soaked in 1 mol / L NaCl for 1 hour, and finally fixed the color with 5% (w / v) acetic acid. The formation of a colorless transparent circle around the colony proved that the bacteria secreted cellulase.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a strain of bacillus licheniformis and its application, belonging to the technical field of biological fermentation and biological transformation. The bacillus licheniformis LZZT‑002 disclosed by the invention has xylanase activity and cellulase activity, and using it for fermentation and conversion of astragalus extract can increase the yield of astragalus polysaccharide, astragalus saponin and derivatives thereof. The fermented astragalus feed additive prepared by fermenting astragalus with bacterial strain LZZT-002 of the present invention is a high-efficiency microbial bacterial agent that integrates probiotics and active ingredients of traditional Chinese medicines, has the characteristics of environmental protection, excellent performance, etc., and has good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of biological fermentation and biotransformation, and more particularly to a strain of Bacillus licheniformis and its application. Background technique [0002] Astragalus is a traditional Chinese medicine. The main medicinal components of Astragalus are astragalus polysaccharides and astragalus saponins, which can improve immune function, have antioxidant, anti-radiation and anti-cancer effects, protect cardiovascular and cerebrovascular, and improve memory. Biocatalysis and transformation technology is an emerging biotechnology that uses the catalytic properties of microbial enzymes to transform known chemical structures into substances with novel structures or higher activity derivatives. Due to the complex components of traditional Chinese medicine, the content of active ingredients is low, the water solubility or stability of some active ingredients of traditional Chinese medicine is not good, and the t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10
CPCA23K10/18C12N1/20C12N1/205C12R2001/10
Inventor 张兴良
Owner LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com