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Subunit f protein of Peste des petits ruminants virus and its preparation method and application

A technology of Peste des petits ruminants and subunits, applied in botany equipment and methods, biochemical equipment and methods, antisense single-stranded RNA viruses, etc., can solve problems such as difficulty in obtaining F protein, non-expression of F protein, and high production costs , to achieve the effect of reducing the risk of dissipating poison, reducing the amount of virus, and solving high production costs

Active Publication Date: 2022-02-11
NOVO BIOTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, subunit vaccines also have obvious shortcomings: high production costs and limited applications
[0007] However, when engineered cells are used to express F protein, if the coding gene sequence of F protein is not optimized, engineered cells basically do not express F protein, and it is difficult to obtain F protein with excellent immunogenicity and stability for small ruminant The prevention and control of zooepidemic virus, therefore, when using engineered cells to express F protein, optimizing the coding gene sequence of F protein is a necessary process

Method used

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  • Subunit f protein of Peste des petits ruminants virus and its preparation method and application
  • Subunit f protein of Peste des petits ruminants virus and its preparation method and application
  • Subunit f protein of Peste des petits ruminants virus and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1a: Structural analysis and optimization of the F protein of Peste des petits ruminants virus was carried out to construct the coding gene sequence of the subunit F protein of Peste des petits ruminants virus.

[0045] By analyzing the F protein sequence of Peste des petits ruminants virus (GenBank: KM091959.1), it can be known that the genome sequence 5532-7172 encodes the F protein sequence of Peste des petits ruminants. Further analysis 1M-19C may be the secretory signal peptide of F protein, 20Q-491N may be the extracellular domain of F protein, 492M-510I may be the transmembrane domain of F protein, 511C-546L may be the intracellular domain protein of F protein . In addition, it was found that the enzyme cleavage site of F protein was between 109R-110F, that is, 20Q-109R was F2 protein, and 110F-491N was F1 protein.

[0046] Combined with the experience of previous studies on the expression of viral envelope proteins, the extracellular region of the subunit F prot...

Embodiment 2

[0234] Example 2: The difference from Example 1 is that in this example, codon optimization is not performed on the coding gene sequence of the subunit F protein, that is, the coding gene sequence of the subunit F protein is shown in SEQ ID NO.2 .

Embodiment 3

[0235] Example 3: The difference from Example 1 is that in this example, codon optimization is performed on the coding gene sequence of the subunit F protein, and its specific coding gene sequence is shown in SEQ ID NO.4.

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Abstract

The invention belongs to the technical field of animal vaccines and veterinary biological products, and particularly relates to a subunit F protein of Peste des Petits Ruminants virus and its preparation method and application. The amino acid sequence of the subunit F protein is: ① as shown in SEQ ID NO. The amino acid sequence shown in 1; ② an immunogenic derived amino acid sequence obtained from SEQ ID NO.1 through substitution, deletion or addition of one or several amino acids. The subunit F protein of the present invention is mainly prepared by constructing a recombinant plasmid, transfecting cell lines with the recombinant plasmid, screening highly expressed cell lines, and purifying the subunit F protein of Peste des petits ruminants virus, which can be better applied to Peste des petits ruminants Viral subunit vaccines or diagnostic reagents have the characteristics of efficient secretion and expression, high protein purity, easy purification, reduced production costs, and high safety performance.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines and veterinary biological products, and in particular relates to subunit F protein of Peste des Petits Ruminants virus and its preparation method and application. Background technique [0002] Peste des Petits of Ruminats (PPR) is a highly contagious viral disease caused by Peste des Petits of Ruminats Virus (PPRV). The World Organization for Animal Health (OIE) classifies it as a Class A infectious disease, and my country also classifies it as a Class I epidemic disease. PPRV mainly infects sheep, especially goats are highly susceptible. Once infected sheep will be characterized by sudden fever, depression, oral ulcers, cough, eye and nose discharge, and diarrhea. The morbidity and mortality can reach 100%, causing huge economic losses and seriously affecting the development of the sheep industry. [0003] At present, there is no effective treatment for the disease, mainly relying on va...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/12C12N15/45C12N15/85A61K39/155A61P31/14G01N33/569
CPCC07K14/005C12N15/85A61K39/12A61P31/14G01N33/56983C12N2760/18422C12N2760/18434G01N2333/12
Inventor 钱泓吴有强卞广林张强徐玉兰吴素芳车影
Owner NOVO BIOTECH CORP
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