Application of F01 polysaccharide for resisting viruses

An anti-virus effect and anti-virus technology, applied in the field of anti-plant virus pesticides and pesticides, can solve the problems of difficult prevention and control of plant virus diseases, no anti-plant virus agents, and no cell structure of plant viruses. The effect of residual drug resistance

Inactive Publication Date: 2020-07-14
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant viruses do not have a complete cell structure, and they are absolutely parasitic to plants. In addition, plants themselves lack a complete immune system. Once a plant is infected by a virus, it will carry the virus for life and suffer continuous damage. The prevention and control has brought great difficulties
At present, there is no specific anti-plant virus agent in agricultural production

Method used

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  • Application of F01 polysaccharide for resisting viruses
  • Application of F01 polysaccharide for resisting viruses
  • Application of F01 polysaccharide for resisting viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Indoor therapeutic effect of F01 polysaccharide on TMV virus.

[0012] (1) Experimental conditions: indoor potted tobacco was selected as the crop, and the variety was Nicotiana cordata; the target was TMV.

[0013] (2) Experimental design and arrangement: the test agent is F01 polysaccharide, and 8% Ningnanmycin water preparation control agent and clear water control are set up. 3 treatments, repeated 3 times.

[0014] (3) Application method: Tobacco heart leaf grows to the 5-6 leaf stage and is used for the test. Using the half-leaf dead spot method, the entire leaf was first inoculated with TMV virus, and 2 hours after inoculation, the left half of the leaf was smeared with F01 polysaccharide or the control agent, and the right half of the leaf was smeared with water as a control.

[0015] (4) Investigation method: 5 days after applying the agent, count the number of dead spots on the left and right half leaves, and calculate the control effect according...

Embodiment 2

[0020] Example 2: Field control effect of F01 polysaccharide on tobacco mosaic virus disease.

[0021] (1) Experimental conditions: field tobacco was selected as the crop, and the variety was K326; the target was TMV.

[0022] (2) Experimental design and arrangement: the test agent is F01 polysaccharide, and 8% Ningnanmycin water preparation control agent and clear water control are set up. 3 treatments, repeated 3 times, a total of 9 plots. The experimental plots were arranged in random blocks.

[0023] (3) Application method: Spray the pesticide 20 days after transplanting the tobacco, and spray the pesticide for the second time after every 7 days.

[0024] (4) Investigation method: Conduct drug efficacy investigation 14 days after the second application of the drug, and record the incidence according to the following standards:

[0025] Grade 0: The whole plant is disease-free; Grade 1: The new leaves show slight mosaic; Grade 2: One-third to one-half of the leaves show ...

Embodiment 3

[0031] Example 3: Field control effect of F01 polysaccharide on wheat yellow dwarf virus disease.

[0032] (1) Experimental conditions: the crop is field wheat, the variety is Xiaoyan 6; the target is BYDV.

[0033] (2) Experimental design and arrangement: the test agent is F01 polysaccharide, and 8% Ningnanmycin water preparation control agent and clear water control are set up. 3 treatments, repeated 3 times, a total of 9 plots. The experimental plots were arranged in random blocks.

[0034] (3) Application method: Spray the pesticide 20 days after the wheat turns green, and spray the pesticide for the second time after every 7 days.

[0035] (4) Investigation method: 14 days after the second spraying, the drug efficacy investigation was carried out. 10 connected wheat plants were investigated at each point, and 250 wheat plants were investigated in each plot. The incidence was recorded according to the following standards:

[0036] Grade 0: No disease in the whole plant;...

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Abstract

The invention relates to a new application of F01 polysaccharide for resisting viruses, in particular to an application of the F01 polysaccharide in preparing a medicine with an antiviral effect. TheF01 polysaccharide disclosed by the invention has prevention and treatment effects on various plant virus diseases, such as tobacco mosaic virus diseases, wheat yellow dwarf virus diseases or rice stripe virus diseases. The F01 polysaccharide disclosed by the invention can be prepared into a pharmaceutical composition and any pesticide acceptable dosage form, such as an aqueous solution, a solubleliquid, missible oil, a microemulsion, a suspending agent, an emulsion in water, wettable powder or a water dispersible granule pharmaceutical composition, by adopting a known method in the prior art. In addition, the F01 polysaccharide has no toxic effect on human, livestock and ecological environment, and is an ideal biopesticide for developing green and organic agriculture.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to the field of anti-plant virus pesticides, and further relates to the new application of polysaccharides acting on anti-viruses. Background technique [0002] Plant virus diseases are an important class of plant diseases. The global economic losses caused by plant virus diseases are as high as 60 billion U.S. dollars every year, of which the loss of food crops alone is as high as 20 billion U.S. dollars. Plant viruses do not have a complete cell structure, and they are absolutely parasitic to plants. In addition, plants themselves lack a complete immune system. Once a plant is infected by a virus, it will carry the virus for life and suffer continuous damage. Prevention and control posed great difficulties. At present, there is no specific anti-plant virus agent in agricultural production. Facing the serious damage of plant virus diseases and the shortage of anti-plant virus ...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P1/00
CPCA01N43/16
Inventor 赵磊张元乐吴云锋
Owner NORTHWEST A & F UNIV
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