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High-F-value oligopeptide and preparation method thereof

An oligopeptide and enzymatic hydrolysis technology, applied in the field of high F value oligopeptide and its preparation, to achieve the effect of improving yield, improving hydrolysis efficiency and improving F value

Inactive Publication Date: 2020-07-24
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the preparation of rice bran high F value oligopeptide

Method used

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Examples

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preparation example Construction

[0020] The invention provides a method for preparing oligopeptides with high F value, comprising the following steps: mixing defatted rice bran and cellulase, enzymolyzing and breaking the wall to obtain protein liquid, and extracting rice bran protein in the protein liquid; The protein is enzymatically hydrolyzed with neutral protease and alkaline protease in sequence to obtain enzymatic polypeptides, and the enzymatic polypeptides are subjected to static adsorption chromatography on activated carbon, centrifuged and dried to obtain oligopeptides with high F value.

[0021] The invention uses defatted rice bran as raw material, mixes the defatted rice bran and cellulase, enzymolyzes and breaks the wall to obtain protein liquid. When the enzymatic hydrolysis of the present invention breaks the wall, it preferably includes mixing defatted rice bran with distilled water, adjusting the pH value to 4.5-5.5, and adding cellulase. When mixing according to the present invention, the ...

Embodiment 1

[0031] Take 8 times the weight of degreased rice bran in distilled water, adjust the pH to 4.5 with 1.0mol / L hydrochloric acid, add 0.3g of cellulase to every 100g of rice bran, hydrolyze it in a water bath at 45°C for 3.5h, then use 1.0mol / L hydrochloric acid at room temperature Adjust pH to 9.0 with L sodium hydroxide, centrifuge at 4500rpm for 20min after stirring for 3h, get supernatant, adjust pH to 4.0 (isoelectric point) with 1.0mol / L hydrochloric acid to carry out PI precipitation; after precipitation is complete, place Centrifuge in a centrifuge at 8000rpm for 20 minutes, wash and dry to obtain rice bran protein, add 10 times the weight of distilled water to the obtained protein, adjust the pH value to 6 with 1.0mol / L sodium hydroxide, add AS1.398 neutral Protease, hydrolyze in a water bath at 40°C for 4 hours, where [E] / [S] is 5%; then use 1.0mol / L sodium hydroxide to adjust the pH value of the hydrolyzate to 11.5, add Alcalase2.4FG alkaline protease at 50°C Hydrolyz...

Embodiment 2

[0033] Take distilled water 10 times the weight of defatted rice bran, adjust the pH to 5 with 1.0 mol / L hydrochloric acid, add 0.4 g of cellulase to every 100 g of rice bran, hydrolyze it in a water bath at 50°C for 4 hours, and then use 1.0 mol / L hydrochloric acid at room temperature Adjust the pH to 9.0 with sodium hydroxide, centrifuge at 5000rpm for 25min after stirring for 2.5h, take the supernatant, adjust the pH to 4.5 (isoelectric point) with 1.0mol / L hydrochloric acid for PI precipitation; after the precipitation is complete, place Centrifuge in a centrifuge at 8500rpm for 15min, wash and dry to obtain rice bran protein, add 11 times the weight of distilled water to the obtained protein, adjust the pH value to 6.5 with 1.0mol / L sodium hydroxide, add AS1.398 neutral Protease, hydrolyze in a water bath at 50°C for 3.5h, where [E] / [S] is 5.5%; then use 1.0mol / L sodium hydroxide to adjust the pH value of the hydrolyzate to 12, add Alcalase2.4FG alkaline protease at 55 Hy...

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Abstract

The invention provides a high-F-value oligopeptide and a preparation method thereof, and relates to the technical field of active peptide preparation. The method comprises the following steps: takingdefatted rice bran as a raw material, firstly adding cellulase for wall breaking, then extracting rice bran protein by an alkaline method, firstly adding neutral protease, then adding alkaline protease for hydrolyzing the protein, removing aromatic amino acid by utilizing activated carbon static adsorption chromatography, and performing separating and drying to obtain the rice bran high-F-value oligopeptide. The F value of the high-F-value oligopeptide is 28-32. The preparation method disclosed by the invention opens up a new direction for the application of the rice bran which is grain leftovers.

Description

technical field [0001] The invention belongs to the technical field of active peptide preparation, and in particular relates to a high F value oligopeptide and a preparation method thereof. Background technique [0002] Rice bran is a by-product of rice milling after husking. It is composed of exocarp, mesocarp, cross-linked layer, seed coat and aleurone layer. It concentrates 64% of the nutritional and functional components of rice and is rich in high-quality protein and fat. , mineral nutrients, B vitamins and vitamin E, etc. Among them, the protein content of rice bran is almost double that of ordinary polished rice (Tao Meng. A new way of comprehensive utilization of rice bran [J]. Practical Technology. 2003, (5): 21.). Rice bran protein is a plant-based protein with rich nutritional value, mainly including albumin, globulin, gluten and prolamin, and the ratio of these four proteins is roughly 37:36:22:5. It is the protein with the lowest allergy among known cereals, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 樊金娟李丛朱延姝阮燕晔崔震海
Owner SHENYANG AGRI UNIV
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