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3D biological printing ink as well as preparation method and application thereof

A bioprinting and 3D printing technology, applied in the field of 3D printing, can solve the problems such as the failure to fully display the biological properties of silk fibroin materials, the reduction of silk fibroin content, the adverse effects of chemical reagents on the long-term growth behavior of cells, and achieve excellent biological properties. , Improve printability, optimize the effect of rheological properties

Active Publication Date: 2020-07-28
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, although the methods currently used by researchers to add chemical reagents or blend other high-viscosity biomaterials can significantly improve the printability of silk fibroin materials, the content of silk fibroin in bioinks prepared by this method often decreases. , failed to fully demonstrate the excellent biological characteristics of silk fibroin materials, and the residue of chemical reagents would have adverse effects on the long-term growth behavior of cells

Method used

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  • 3D biological printing ink as well as preparation method and application thereof
  • 3D biological printing ink as well as preparation method and application thereof
  • 3D biological printing ink as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] In this embodiment, a preparation method of 3D bioprinting ink for 3D bioprinting bone defect scaffold is provided, the method comprises the following steps:

[0051] Step 1, preparing silk fibroin aqueous solution;

[0052] Silk fibroin aqueous solution is obtained after degumming, dissolving, dialysis and centrifugation. Degumming, dissolving, and dialysis processes all adopt common technical means in this field. For details, reference can be made to the literature Rockwood D N, Preda R C, Yücel, Tuna, et al.Materials fabrication from Bombyx mori silk fibroin[J].Nature Protocols,2011,6(10) :1612-1631.. Wherein, the molecular weight cut-off of the dialysis bag used in the dialysis process is 3500kDa. The concentration of the finally prepared silk fibroin aqueous solution is 10wt%.

[0053] Step 2, the silk fibroin aqueous solution is made into a silk fibroin electrogel under the action of a stable electric field;

[0054] The silk fibroin aqueous solution prepared ...

Embodiment 2

[0059] In this example, the pure silk fibroin 3D bioprinting ink 3D printing gel scaffold prepared in Example 1 is provided.

[0060] Put the pure silk fibroin 3D bioprinting ink prepared in Example 1 on the 3D printer, open the 3D printer control panel, set the temperature of the nozzle to 5°C, and place the pure silk fibroin 3D bioprinting ink on the nozzle for temperature pre-control for 15 minutes , so that the pure silk fibroin 3D bioprinting ink reaches the preset nozzle temperature. Use the platform to receive the printed holder, and the temperature of the platform is set to 5 °C. 3D print the pure silk fibroin gel scaffold according to the model set by the 3D printer, see the 3D printed pure silk fibroin gel scaffold Figure 5 , the number of layers is 10.

Embodiment 3

[0062] In this embodiment, the application of a pure silk fibroin 3D printing scaffold in 3D bioprinting bone defect materials is provided, including:

[0063] The pure silk fibroin gel scaffold prepared in Example 2 was frozen at -20°C for four to five hours, and then placed in a freezer at -80°C for overnight freeze-drying to obtain pure silk fibroin 3D printing Freeze-dried scaffolds.

[0064] Using scanning electron microscope (SEM) to observe the above-prepared 3D printed scaffold, the freeze-dried scaffold has a micron-scale microporous structure inside, and its structure is detailed in Figure 6 .

[0065] The 3D printing scaffold prepared above was used for in vitro cell culture. The cultured cells were pre-osteoblast MC3T3 cells, and the cultured cells were labeled with fluorescent proteins. Figure 7 It is the confocal image of the 7th day of cell culture. It can be seen from the figure that a large amount of fluorescence is displayed inside and on the surface of t...

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Abstract

The invention relates to a 3D biological printing ink as well as a preparation method and application thereof. The preparation method comprises the following steps: applying a constant voltage of 50 Vor less to a silk fibroin water solution for 120 min or less to obtain silk fibroin electro-gel, removing bubbles in the silk fibroin electro-gel at 30-100 DEG C, and cooling to obtain the 3D biological printing ink. The biological printing ink is suitable for a 3D printing technology, is prepared from pure silk fibroin, and does not contain other chemical reagents; the obtained pure silk fibroinprinting ink has good 3D printability and excellent biocompatibility, the cross-linking mode after printing and forming is simple and convenient, and the mechanical property of a crosslinked stent isgood.

Description

technical field [0001] The invention relates to the technical field of 3D printing, in particular to a 3D bioprinting ink and its preparation method and application. Background technique [0002] Three-dimensional (3D) printing technology is an emerging manufacturing technology developed based on the principle of layered manufacturing. It forms a 3D structure in a layer-by-layer superimposed manner through computer control. Biological 3D printing technology is a branch of 3D printing technology. 3D bioprinting is a comprehensive discipline that combines computer science, cell biology, materials science and medicine. Bio-3D printing ink, referred to as bio-ink, is a key component of bio-3D printing. The ideal bio-ink should have good printability, biocompatibility, degradability, side effects, and suitable mechanical properties. Due to the high requirements for bioprinting inks, currently available bioinks for different 3D bioprinting technologies have become an obstacle re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D11/30C09D11/04A61L27/22A61L27/50A61L27/52B29C64/112B33Y10/00B33Y70/00
CPCC09D11/30C09D11/04A61L27/227A61L27/50A61L27/52B29C64/112B33Y10/00B33Y70/00A61L2430/02C08L89/00
Inventor 王卉陈晓敏张克勤
Owner SUZHOU UNIV
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