Gene for improving cyst nematode resistance of soybeans and application of gene

A cyst nematode and gene technology, which is applied in the field of genes for improving soybean resistance to cyst nematode disease, can solve the problem that the number of cyst nematodes in the roots of susceptible soybean plants is large, the disease resistance operation is difficult, and the technical cost is high. problems, to achieve the effect of improving breeding efficiency, saving manpower and material resources, and reducing the number of females
CN111471689AActive Publication Date: 2020-07-31NORTHEAST AGRICULTURAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2020-07-31

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Abstract

The invention discloses a gene for improving cyst nematode resistance of soybeans and application of the gene, and belongs to the technical field of genetic breeding. Aiming at the problems that: thenumber of cyst nematode female insects in infected soybean plants is large, conventional disease-resistant operation difficulty is high at present, and the technical cost is high, the invention provides the gene for improving cyst nematode resistance of soybeans. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1. An Agrobacterium rhizogenes K599 mediated genetic transformation methodis adopted, pCSXN1250-GmRSCN16-1 recombinant plasmid is transferred into an infected Dongnong 50 plant, rooting is induced, physiological races of soybean cyst nematode No.1, No.3 and No.4 are inoculated in a mixed manner, and the invention proves that the GmRSCN16-1 gene has obvious resistance to the soybean cyst nematode. The gene can be used for breeding soybean varieties with the cyst nematode resistance.
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Description

technical field

[0001] The invention belongs to the technical field of genetic breeding, in particular to a gene for improving the resistance of soybean to cyst nematode disease and its application. Background technique

[0002] The phenomenon of continuous soybean cropping exists widely in the world, and the number of cysts in soil and roots will increase with the increase of planting years in soybean continuous cropping areas. Although the control effect of applying pesticides in the soil to treat seeds is obvious, the amount of pesticides used is very large. Long-term use will easily cause nematodes to develop pesticide resistance and cause great environmental pollution. Regarding the control of this disease, the most effective way is to plant and breed soybean varieties that are not sensitive to it. When breeding disease-resistant varieties, conventional breeding methods are time-consuming, about 7 to 10 years, and require a large area of ​​breeding fields, consuming a ...

Claims

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