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Separation and purification process and device for bacca sedge polysaccharide

A technology for separation and purification of red barnyard, applied in the field of raw material extraction, can solve the problems of high loss rate of red barnyard polysaccharide, low extraction rate, etc., and achieve the effects of good separation and purification effect, increase extraction rate, and avoid raw material waste.

Inactive Publication Date: 2020-09-01
ZUNYI NORMAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the extraction technology of red barnyard polysaccharides in the prior art is too conventional, the extraction rate is low, and the loss rate of red barnyard polysaccharides is relatively high in the process of purifying and removing proteins. Therefore, the present invention designs a separation and purification process of red barnyard polysaccharides and its device

Method used

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  • Separation and purification process and device for bacca sedge polysaccharide
  • Separation and purification process and device for bacca sedge polysaccharide
  • Separation and purification process and device for bacca sedge polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: a kind of separation and purification process of red barnyard barnyard polysaccharide mainly comprises the following steps:

[0038] S1: Preprocessing

[0039] Take red barnyard fruit and place it in 0.1mol / L dilute hydrochloric acid solution, ultrasonically soak it in ultrasonic cleaning machine 1 for 2 hours, rinse the red barnyard fruit after soaking with deionized water twice to remove the sticky impurities of red barnyard fruit, and then Dry at 60°C for 4-5 hours to break the shell of the red barnyard fruit, then use the gravity sorter 2 to remove the shell of the red barnyard fruit, send the shell-removed red barnyard fruit to the grinder 3 for crushing, and after crushing The red barnyard fruit is passed through a 100-mesh sieve, dried at 80°C for 2 hours, and red barnyard powder is obtained after degreasing;

[0040] The specific process of degreasing is: dissolving red barnyard powder and deionized water in the extraction tank 4 according to the ma...

Embodiment 2

[0048] It is basically the same as Embodiment 1, except that in S2, the positive and negative poles of the high-voltage pulse extraction device 5 are alternated before each high-voltage pulse extraction,

[0049] Add the β-glucan clathrate solution to the first extraction solution, repeat the above high-pressure pulse extraction step, and the extraction time is 5 minutes, to obtain the second extraction solution;

[0050] Add the β-glucan clathrate solution to the second extraction solution, repeat the above-mentioned high-pressure pulse extraction step, and the extraction time is 10 minutes, to obtain the third extraction solution;

[0051] Add the β-glucan inclusion compound solution to the third extraction solution, repeat the above-mentioned high-pressure pulse extraction step, and the extraction time is 40 minutes to obtain the fourth extraction solution. The volume ratio of the β-glucan inclusion compound solution added three times is is 1:3:7,

[0052] The preparation ...

experiment example 1

[0058] Experimental Example 1: Studying the Effect of β-Glucan Inclusion Complex on the Extraction Efficiency of Red Barnyard Polysaccharide

[0059] Using the process provided in Example 1 and Example 2 to separate and extract the same amount of red barnyard polysaccharides, respectively prepare red barnyard polysaccharide A and red barnyard polysaccharide B, and measure the red barnyard polysaccharides of embodiment 1 and embodiment 2 by the following formula Extraction rate:

[0060] Red barnyard polysaccharide extraction rate = red barnyard polysaccharide mass / red barnyard fruit mass * 100%,

[0061] Calculate respectively the red barnyard barnyard polysaccharide extraction rate of embodiment 1, embodiment 2, and the results are as shown in table 1:

[0062] The red barnyard barnyard polysaccharide extraction rate test result table of table 1 embodiment 1, embodiment 2

[0063] group Red barnyard fruit mass / g Red barnyard polysaccharide mass / g Extractio...

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Abstract

The invention provides a carex baccans polysaccharide separation and purification process and a carex baccans polysaccharide separation and purification device. The carex baccans polysaccharide separation and purification process comprises the following steps: S1, ultrasonically soaking carex baccans fruits, drying, removing shells, crushing and degreasing to obtain carex baccans powder; s2, dissolving carex baccans nees powder in deionized water, performing alternate high-voltage pulse extraction in a high-voltage pulse extraction device, adding a beta-glucan inclusion compound solution intothe pre-extracting solution before extraction, performing centrifugal treatment on the extracting solution, taking supernatant, performing filtration, and performing vacuum drying to obtain carex baccans nees crude polysaccharide; s3, decoloring the bacca sedge crude polysaccharide, dissolving the decolored bacca sedge crude polysaccharide in deionized water, adding protease for reaction, adding achloroform-n-butyl alcohol mixed solution, performing oscillation reaction, performing centrifugal treatment to remove protein gel, and drying to obtain bacca sedge polysaccharide. In conclusion, themethod has the advantages of complete process, advanced device, good separation and purification effect and the like.

Description

technical field [0001] The invention belongs to the technical field of raw material extraction, and in particular relates to a separation and purification process of red barnyard polysaccharide and a device thereof. Background technique [0002] Red barnyardgrass is a wild endangered crop that grows in the cold sandy soil at an altitude of 1200-1500 meters in Guizhou. After artificial domestication and cultivation, it has become a unique crop in Guizhou. However, due to the extremely low yield, it has been abandoned and forgotten by many people. "Compendium of Materia Medica", "Compendium of Chinese Herbal Medicine", "Ancient Southern Yunnan Materia Medica" are all recorded. [0003] Red barnyardgrass has excellent medicinal and edible effects. It can supplement rich nutrition for the human body, nourish blood, activate blood, regulate menstruation and stop bleeding, which is of great benefit to maintaining human health. [0004] Polysaccharide (polysaccharide) is a polysa...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张相春杨亚静高智席刘军龙敖克厚
Owner ZUNYI NORMAL COLLEGE
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