Glucose oxidase nanocapsule sensor and preparation and application thereof

A technology of glucose oxidase and nanocapsules, which is applied in the field of biosensors and analysis and detection, and can solve the problems of undiscovered enzyme molecule nanocapsules

Active Publication Date: 2020-09-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] We searched relevant literature and patents at home and abroad, although there are single-protein nanocapsules for protein delivery with long-term effects (patent application number 201080020405.1), oral delivery of enzymes using nanocapsules to achieve targeted metabolism of alcohol or toxic metabolites (Patent Application No. 201280043352.4), an antioxidative enzyme nanocapsule aerosol and its application in eliminating smoke free radicals (Application Publication No. CN104549085A) related patent applications and literature reports, but no enzyme molecular nanocapsules were found In particular, relevant literature reports and patent applications of glucose oxidase nanocapsules used in enzyme sensors

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  • Glucose oxidase nanocapsule sensor and preparation and application thereof
  • Glucose oxidase nanocapsule sensor and preparation and application thereof
  • Glucose oxidase nanocapsule sensor and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Preparation of Glucose Oxidase Molecular Nanocapsules (nGOx)

[0047] Glucose oxidase molecular nanocapsules (nGOx) can be prepared by methods such as in situ free radical polymerization, and its preparation includes two typical processes. figure 1 A typical flow chart for the preparation of glucose oxidase molecular nanocapsules is given in . First, in the first step, in a buffered saline solution (or organic solvent) of glucose oxidase at an appropriate concentration, the organic molecule monomer (such as N-propylene) with a double bond (alkenyl) is absorbed by adsorption, covalent cross-linking, etc. base succinimide) to the surface of the enzyme molecule to make the enzyme a single molecule of enzyme with a polymerizable group (double bond) (the enzyme wrapped by small molecules is distributed in a single molecule in the solution); then in the second step, add at least An organic monomer molecule with a double bond (such as acrylamide, etc.) and / or a cross-linking...

Embodiment 2

[0051] Fabrication of nGOx-based biosensors

[0052] In this study, bio(nano)sensors (modified electrodes) based on nGOx and GOx were prepared by the drop coating method, and the typical preparation scheme is as follows: figure 2 shown. figure 2 The scenario presented is one of many possible scenarios and is only an example. In actual operation, various procedures, methods and components can be used to prepare biosensors. For example, a mixed solution of various components can be used directly for the preparation of modified electrodes (without the need for figure 2 The modified electrode is also prepared by a step-by-step method), or the modified electrode is prepared by screen printing, 3D printing, etc.; the electrode substrate can sample many materials such as glassy carbon electrodes, glass electrodes, metal electrodes, and test paper electrodes; the sampled nanomaterials play a role To improve the sensitivity, you can choose many nanomaterials such as ordinary carbo...

Embodiment 3

[0056] Structure and morphology characterization of nGOx

[0057] The particle size distribution and zeta potential (zeta potential) of nGOx and native GOx molecules were measured by dynamic light scattering (DLS) method. The natural GOx molecule presents a negative charge of −9.6 mV, and the particle size distribution is centered at about 10.1 nm. The average particle size distribution of the as-prepared nGOx molecules was 15.6 nm, confirming the successful formation of a thin polymer protective layer with a thickness of about 2–3 nm around each GOx molecule ( image 3 A). The nGOx molecules exhibit less negative charge of −6.0 mV due to the encapsulation of the polymer layer.

[0058] image 3 B The transmission electron micrograph (TEM) shows that the nGOx nanocapsules have a spherical shape, and the average particle size is about 16nm, which is consistent with the test results of the DLS method. also, image 3 The UV-vis spectra of nGOx and GOx are given in C. The GO...

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Abstract

The invention discloses a glucose oxidase nanocapsule sensor and preparation and application thereof. An enzyme-based biosensor has a wide application prospect in the fields of biomedical treatment, life health, food safety, environmental safety, food fermentation and the like, and the main factor for limiting commercialization of the enzyme-based biosensor at present is that the stability of theenzyme sensor needs to be further improved. The invention provides a preparation method and application of a high-stability enzyme sensor, namely a method for preparing the enzyme sensor based on an enzyme molecule nanocapsule. The preparation method of the enzyme nanocapsule sensor mainly comprises the following steps: firstly, preparing an enzyme monomolecular nanocapsule (or an enzyme polymolecular nanocapsule) by using an in-situ free radical polymerization method; and then, using the enzyme molecule nanocapsule to prepare the enzyme nanocapsule sensor. The enzyme sensor has the advantagesthat the thermal stability, the organic solvent tolerance, the storage life, the service life and the like of the enzyme sensor are obviously improved. The enzyme nanocapsule sensor obtained by the invention has huge market application potential in the fields of human body wearable equipment, biomedical treatment, analysis and detection, food fermentation and the like.

Description

technical field [0001] The invention belongs to the field of biosensors and analysis and detection, and in particular relates to a sensor based on enzyme nanocapsules including glucose oxidase nanocapsules, a preparation method thereof, and applications in the fields of glucose detection and human wearable devices. Background technique [0002] Blood glucose testing is fundamental to the diagnosis, treatment and management of diabetes. At present, there are more than 420 million people with diabetes in the world. In 2015, the number of people with diabetes in China alone was as high as 109 million. The global market capacity of blood glucose meters and blood glucose test strips (that is, enzyme-based electrochemical glucose sensors) is as high as more than 20 billion US dollars. At present, there are two main types of blood glucose test strips on the market: one is the glucose oxidase (GOx) electrode measurement method, which is dominant and indicates the blood glucose conc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327A61B5/1486
CPCG01N27/327G01N27/3272A61B5/1486A61B5/14532A61B5/6802
Inventor 卢宪波郸嘉陈吉平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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