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Cyclic gamma-polyglutamic acid modified hydrogel loaded nano sponge detoxification system and preparation method thereof

A polyglutamic acid and nano-sponge technology, applied in medical science, bandages, etc., can solve problems such as unreported, and achieve the effect of good biocompatibility

Inactive Publication Date: 2020-09-11
中国人民解放军海军特色医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report about the detoxification system and preparation method of cyclo-γ-PGA modified PNIPAM hydrogel loaded with NS

Method used

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  • Cyclic gamma-polyglutamic acid modified hydrogel loaded nano sponge detoxification system and preparation method thereof
  • Cyclic gamma-polyglutamic acid modified hydrogel loaded nano sponge detoxification system and preparation method thereof
  • Cyclic gamma-polyglutamic acid modified hydrogel loaded nano sponge detoxification system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the preparation of NS

[0047] ①Preparation of PLGA nano-core: 0.67dl / g carboxyl-containing 50:50 PLGA monomer polymer was prepared into 60nm PLGA nanoparticles by nano-precipitation method. First, dissolve PLGA monomer in acetone to prepare PLGA acetone solution (5mg / ml); then, add 1ml PLGA acetone solution to 3ml deionized water, stir at room temperature for 2h; finally, centrifuge with AmiconUltra-4 with a molecular weight cut-off of 10kDa The filter was filtered to obtain PLGA nanoparticles ( figure 1 ). In addition, to prepare fluorescently labeled NSs for subsequent tracking, 0.1 wt% Did fluorescent dye was added to PLGA acetone solution before polymerization, and Did-labeled PLGA nanoparticles were similarly prepared ( figure 1 ). Observed under the transmission electron microscope, it can be seen that the typical nano-scale particle structure ( figure 2 ). The hydrated particle size and zeta potential ( image 3 , 4).

[0048] ②Preparation ...

Embodiment 2

[0050] Example 2: Preparation of cyclo-γ-PGA modified PNIPAM hydrogel loaded NS detoxification system

[0051]①cyclo-γ-PGA modified PNIPAM hydrogel: mix the two substances in sequence according to PNIPAM / cyclo-γ-PGA ratio of 100:0, 75:25, 50:50, and 25:75, and the total concentration of the solution is 20% ( w / v), mix well at room temperature for 3 hours, remove air bubbles overnight at 4°C, and gel in a water bath at 37°C. The dehydration phenomenon of PNIPAM hydrogel can be observed when PNIPAM / cyclo-γ-PGA is 75:25 or 50:50. effectively improved ( Figure 7 ,8). Under the scanning electron microscope, it was observed that when the ratio of PNIPAM / cyclo-γ-PGA was 75:25, the hydrogel showed a typical porous structure ( Figure 9 ).

[0052] ②Mix the final concentration of 2mg / ml NS, 150mg / mL PNIPAM and 50mg / mLcyclo-γ-PGA, mix thoroughly at room temperature for 3 hours, remove air bubbles overnight at 4°C, and gel in a water bath at 37°C. The structure of hydrogel-loaded NS...

Embodiment 3

[0053] Example 3: Biocompatibility of cyclo-γ-PGA modified PNIPAM hydrogel loaded NS detoxification system

[0054] To detect the cytotoxic effect of cyclo-γ-PGA modified PNIPAM hydrogel loading NS detoxification system. First, prepare cyclo-γ-PGA modified PNIPAM hydrogel-loaded NS detoxification system extract, immerse the prepared detoxification system in serum-free DMEM medium at a ratio of 0.1 g / mL, and extract at 37°C for 24 hours , collect the culture medium, and pass through a 0.22 μm filter membrane to sterilize for later use. After cell counting, L929 cells were divided into 1 × 10 4 Inoculate each well into a 96-well plate, cultivate overnight until it adheres to the wall, suck away the medium, then dilute the extraction solution according to the concentration gradient of 100%, 50%, and 25%, add 100 μL of different concentration extraction solutions to each well, and cultivate for 24 hours or 48h, after taking it out, add 10μL CCK-8 reagent to each well, incubate f...

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Abstract

The invention relates to the field of nano medicine and biological detoxification, in particular to a cyclic gamma-polyglutamic acid modified poly-N-isopropylacrylamide hydrogel loaded nano sponge detoxification system and a preparation method thereof. PLGA nanoparticles are wrapped with erythrocyte membrane vesicae prepared from extracted erythrocyte membranes to prepare nano sponge, and cyclic gamma-polyglutamic acid modified poly-N-isopropylacrylamide hydrogel is loaded with the nano sponge to obtain the system. The prepared hydrogel of a three-dimensional network structure has good biocompatibility, can be loaded with the NS to form the detoxification system with the local toxin adsorption effect, and can be applied to the fields of biological medicines, medical auxiliary materials andthe like.

Description

technical field [0001] The invention relates to the technical field of nanomedicine, in particular to a ring gamma-polyglutamic acid modified hydrogel-loaded nano-sponge detoxification system and a preparation method thereof. Background technique [0002] At present, bacterial infection is still a class of diseases with high morbidity and high mortality worldwide. Due to the widespread use of antibiotics and stagnation in the development of new antibiotics, drug-resistant bacteria, especially "super bacteria" have increased in recent years, seriously threatening human health. Pore ​​forming toxins (PFTs) are the main virulence factors of bacterial infection, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, some Vibrio )(Blake KJ,Baral P,Voisin T,et al:Staphylococcus aureus produces pain through pore-formingtoxins and neuronal TRPV1that is silenced by QX-314.Nat Commun 2018,9(1):37;Van Pee K,Mulvihill E, Muller DJ, et al: Unraveling the pore-forming steps ...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0014A61L26/0019A61L26/0057A61L26/0061A61L26/008A61L26/0085C08L33/26C08L77/04
Inventor 张黎明王蓓蕾邹帅军王超王倩倩柳国艳王博张福海
Owner 中国人民解放军海军特色医学中心
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